A Research Technician At Memorial Sloan Kettering Cancer Center

1043 Words Sep 6th, 2016 5 Pages
Since my previous application, I have continued working as a research technician at Memorial Sloan-Kettering Cancer Center. I examine the epigenetic changes that often occur in the progression of cancers. Using techniques such as Chromatin Immunoprecipitation and Assays for Transposase Accessible Chromatin follow by high throughput sequencing (ChIP-seq and ATAC-seq) , I focused on alterations in histone modifications and changes in chromatin accessibility. Since I collaborated with other investigators, I was able to study a variety of different cancer types including breast cancer, gliomas, various leukemias and prostate cancer. I work with mouse models as well as actual patient samples. I have been involved in a clinical trial for a small molecule inhibitor of the histone methyltransferase DOT1L as a treatment for myeloid leukemia in both adult and pediatric patients. I performed ChIP-seq on patient peripheral blood and bone marrow sample to measure the level of H3K79 dimethylation globally and at specific target genes. The experimental data I help generate aid physicians in determining if the drug is working as expected at a molecular level and whether H3K79 dimethylation level correlates with clinical outcomes. I will likely be involved in more clinical trials in the coming year for other small molecule inhibitors as treatment of various leukemias. In addition, I have been volunteering at the Hospital for Special Surgery where I am a Surgical Liaison. I help explain to…

Related Documents