01a Membrane Diffusion Lab Report

To begin this experiment, make sure that the laptop computer is turned on and connect the Conductivity Probe to the computer interface. Set the Conductivity Probe to the intermediate setting, 2000 μS/cm (0 to 1000 mg/L TDS). Open the file “01A Membrane Diffusion” from the Advanced Biology with Vernier folder of Logger Pro to prepare for data collection. The first portion of this experiment will test whether the different concentration gradients affect the rate of diffusion. Three different concentrations of salt solution will be used to demonstrate the different concentration gradients. Predicted outcomes, along with the conclusions, of the experiment will be recorded in Data Table 1. Begin by making up the salt solutions needed for the experiment. To make …show more content…
Once the tubing is filled repeat the previous procedure for tying the tubing and secure the top end of the tubing. Thoroughly wash the outside of the tubing with tap water to ensure that there is no salt adhering to the tubing. Fill the 400ml beaker with 300 ml of DI water. Lower the Conductivity Probe into the water, avoiding contact with the sides and bottom of the beaker. Place the dialysis tubing into the beaker and making certain that the tubing is entirely submerged in the water. It is important to place the dialysis tubing and the Conductivity Probe the same distance from one another for each test. With the stirring rod, stir the solution for 30 seconds, avoiding contact with the probe or tubing, before collecting data. Once the 30 seconds has passed begin collecting data by clicking on the “collect” box in the computer software. Continue to stir the solution for the entire two minutes of data collection. After two minutes have passed the data collection will automatically stop. To determine the rate of diffusion locate the area on the graph where the data values begin to increase. Click on this area with the mouse and drag the pointer to the end of the data