Microbiology Lab Exam Ii

Front 1

Why were the IMVic tests developed?

Back 1

As a means of separating members of the Enterobacteriaceae

Front 2

What are the letters of IMVic for?

Back 2

I for indole production from tryptophan
M for methyl red test for acid production from glucose
V for the Voges-Proskauer test for production of acetoin from glucose
C for the use of citrate as the sole carbon source

Front 3

What was the source of carbon Lab #14 Rapid Identification Methods?

Back 3

citric acid

Front 4

If the indicator turns from green to blue what does this indicate?

Back 4

Conditions are alkaline

Front 5

Characteristics of Enterobacteriaceae

Back 5

aerobic or facultatively anaerobic
gram negative
nonendospore-forming
rod-shaped

Front 6

What are coliforms?

Back 6

enterobacteriaceae that ferment lactose with acid and gas formation within 48 hours at 35 degrees celsius

Front 7

What is Enterotube II

Back 7

a rapid identification method that identifies oxidase-negative, gram-negative bacteria belonging to the family enterobacteriaceae

Front 8

Antimicrobial agents

Back 8

chemicals that control the growth of microbes

Front 9

Disinfectants

Back 9

chemical agents used on inanimate objects to lower the level of microbes on their surface

Front 10

What are antiseptics?

Back 10

chemicals that are used on living tissue to decrease the number of microbes

Front 11

Bactericidal agents

Back 11

chemical agents that result in bacterial death

Front 12

Bacteriostatic agents

Back 12

chemical agents that cause temporary inhibition of growth

Front 13

Factors to consider in selecting an antimicrobial agent

Back 13

pH
solubility
toxicity
organic material present
cost

Front 14

What factors are important in evaluating the effectiveness of antimicrobial agents?

Back 14

the concentration
length of contact
whether it is lethal or inhibiting

Front 15

American Official Analytical Chemist's use dilution test

Back 15

standard method for measuring the effectiveness of a chemical agent

Front 16

What culture was used in lab 17-Disinfectants and Antiseptics

Back 16

Staphylococcus aureus

Front 17

Louis Pasteur

Back 17

made the observation that some microbes inhibited the growth of others in 1874

Front 18

Antibiosis

Back 18

against life

Front 19

What are chemotherapeutic agents?

Back 19

antimicrobial chemicals absorbed or used internally whether natural (antibiotics) or synthetic

Front 20

Minimum inhibitory concentration (MIC)

Back 20

the concentration of chemotherapeutic agent at the edge of the zone of inhibition

Front 21

What is the zone of inhibition size affected by?

Back 21

the diffusion rate of the chemotherapeutic agent and the growth rate of the organism

Front 22

In Lab 18-Antimicrobial Drugs, which plates and broth were used? How did you get from ml to cm?

Back 22

Mueller Hinton agar
pseudomonas aeruginosa broth
multiplied by 10 to get from ml to cm

Front 23

Ignaz Semmelwies

Back 23

established a policy that resulted in the drop in the death rate due to puerperal sepsis from 12% to 1.2% in one year

Front 24

Handwashing in hospitals

Back 24

as low as 31%

Front 25

In Lab #19 Hand Scrubbing, which plates were used?

Back 25

used blood agar plates

Front 26

Upper respiratory system

Back 26

nose and throat

Front 27

Lower respiratory system

Back 27

larynx, trachea, bronchial tubes and alveoli

Front 28

Why is the lower respiratory tract normally sterile?

Back 28

because of the efficient functioning of the ciliary escalator

Front 29

Microbial antagonism

Back 29

certain microorganisms of the normal microbiota suppress the growth of other microorganisms through competion for nutrients and production of inhibitory substances

Front 30

What are the predominant organisms in throat cultures?

Back 30

streptococcal species

Front 31

B-hemolysis

Back 31

beta (bad-beta ate it)
complete hemolysis giving a clear zone with a clean edge around the colony

Front 32

a-hemolysis

Back 32

incomplete hemolysis, producing methemoglobin and a green cloudy zone around the colony (good)

Front 33

What characterizes gamma-hemolysis?

Back 33

no hemolysis and no change in the blood agar around the colony

Front 34

S. pyogenes

Back 34

90% of streptococcal infections are caused by b-hemolytic group A strepcocci
sensitive to bacitracin

Front 35

Optochin sensitivity

Back 35

used to distinguish pathogenic S. pneumoniae from other a-hemolytic streptococci

Front 36

Strep

Back 36

does not bubble

Front 37

Resident bacteria of urethra

Back 37

streptococcus, bacteroides, mycobacterium, neisseria and enterics

Front 38

Bacteria in urine

Back 38

not considered an indication of urinary tract infection unless there are 1000 bacteria of one species or 100 coliforms per milliliter of urine

Front 39

Cystitis

Back 39

inflammation of the urinary bladder

Front 40

Pyelonephritis

Back 40

inflammation of the kidney

Front 41

Urine sample

Back 41

must be a clean catch, midstream into sterile jar

Front 42

Bacteria of Urogenital tract

Back 42

special 10ul calibrated loop was used instead of regular inoculating loop

Front 43

In Bacteria of Urogenital tract, which plates were used?

Back 43

used blood agar plate and MacConkey agar-which turns pink if growing enterics

Front 44

P. aeruginosa infections

Back 44

are characterized by blue green pus

Front 45

Gonorrhea

Back 45

The most common reportable communicable disease in the US, caused by the gram negative diplococci Neisseria gonorrhoeae

Front 46

In Chemical Methods of Control: Antimicrobial Drugs, which plates were used?

Back 46

used Mueller-Hinton agar plates

Front 47

In Chemical methods of Control: Antimicrobial Drugs, which cultures were used?

Back 47

used cultures
Staphylococcus aureus
Escherichia coli
Pseudomonas aeruginosa

Front 48

What was the purpose of Chemical methods of Control: Disinfectants and Antiseptics

Back 48

purpose was to time exposure of staphylococcus aureus to 10% bleach and other disinfectants and measuring growth

Front 49

Enterics

Back 49

oxidase negative and feed on glucose

Front 50

Procedure for Rapid Identification Methods

Back 50

1.pick a well isolated colony with the inoculating end of wire
2. hold the bent end of the wire and withdraw the needle through all twelve compartments with a turning motion
3.reinsert the wire through all twelve compartments. Then withdraw it to the notch on the wire. Break the wire at the notch.
4. Using the broken wire punch holes through the foil covering the air inlets in the last eight compartments. Replace caps

Front 51

Citrobacter freundii

Back 51

- Indole
+ Methyl red
- Voges-Proskauer
+ Citrate

Front 52

Enterbacter aerogenes

Back 52

- Indole
-Methyl red
+ Voges Proskauer
+ Citrate

Front 53

Serratia marcescens

Back 53

-Indole
+ or - for methyl red
+ Vokes Proskauer
+ citrate

Front 54

Proteus vulgaris

Back 54

+ Indole
+ methyl red
- Voges proskauer
-(v) Citrate

Front 55

Bacillus subtillis

Back 55

catalase positive

Front 56

Staph

Back 56

catalase positive

Front 57

Strep

Back 57

catalase negative

Front 58

In Respiration lab #13, which plate was used?

Back 58

TSA agar plate

Front 59

Steps for Isolation of Bacteria by Dilution Techniques

Back 59

streak first sector making as many streaks as possible
turn plate to next sector and streak
turn plate to next sector and streak
turn plate to next sector and streak
DO NOT OVERLAP or FLAME IN BETWEEN steps!