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34 Cards in this Set
- Front
- Back
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Making Agar Steps
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*sterilize
*Prepare agar *Place the petri dishes the right way up and crack but do not remove the lids *remove any chance of airborne germs entering *Pour an amount of agar liquid into each *Allow the agar to set |
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what is fine adjustment to keep focused without adjusting light
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Parfocal
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Resolving Power (distance)
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.55*2(NA)
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What is numerical aperture
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light trapping ability
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Micrometer
Nanometer |
10^-6
10^-9 |
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Euglena
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*most like a protozoa
*Photosynthetic like algae |
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Euglenoid
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*new classification
*developed due to inability to place in algae or protazoan |
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Prokaryotes
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*single celled
*lack membrane bound nucleus and organelles |
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Cyanobactrium
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*once blue algae
*single celled *arranged in colonies *photosynthetic |
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Anabeana
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*HETEROCYST
-larger, clear in live prep -stores fixed oxygen |
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Gleocapsa - what is the capsule used for?
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*good aquatic indicator
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Eubacteria
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*true bacteria
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Bacillus
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*rods
*Arrangements: stacked, in chains, irregular |
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Coccus
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*Spherical
*Arrangements: dipplococcus, tetrads of cocci, streptococcus, staphylococcus |
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Spirillium
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*Helical
*Irregular |
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Giardia Lamblia
Symtoms? Why must you filter AND boil water |
*Symptoms
-Diarrhea *filter and boil to destroy cyst stage |
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Tryoanosoma Brucei Gambiense
Vector? What are later stage symptoms? |
*vector
-tsetse fly *late symptoms - uncontrollable sleepiness, coma |
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Paramecium
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*take in food by oral groove
*good aquatic indicator |
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Algae
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Single OR multicellular
*produce sugar and oxygen *good environmental indicators |
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Spirogyra
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*spiral shaped organelles
-chloroplasts |
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Diatoms
What is light traping pigment? What is silica in cell walls used for? |
*light trapping
-xanthophyll *used in diatomaceous earth |
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TSA plates made of
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soy casing & salt component
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what temp does agar solidify?
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40-45c
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what temp does agar liquify?
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90-95c
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What
temp pressure time is media autoclaved? |
*121c
*15 PSI above atomsphere pressure *15-20 minutes |
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Why are plates incubated in inverted position?
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condesation prevention
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why have a control plate?
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check sterility
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why is petri dish from skin sample incubated at 37c
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its close to out body temeprature
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Gram stain 4 steps
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cover with
-violet stain (1min) -grams iodine (1min) -grams alcohol (1min) -safranin (30sec) |
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what happens if you over decolorize?
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bacteria will appear pink, false negative
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Peptoglycan colors what color?
Thin is what color? |
*Peptoglycan - thick layer purple
*thin doesnt bind, negative pink |
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Technique of streak plate?
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isolate colony
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best part of flame?
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blue part
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why was serratia marcencens incubated at room temp?
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slows enzymes in pigmentation
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