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41 Cards in this Set

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At the begining of each lab period. Disinfect the _________ top with_____________. Wash hands with _________ and ___________.
At the begining of each lab period. Disinfect the bench top with disinfectant. Wash hands with soap and water.
At the end of each lab period. __________ the bench top with _____________. Wash __________ with _________ and hot __________.
At the end of each lab period. Disinfect the bench top with disinfectant. Wash hands with soap and hot water.
Steps if a bacterial culture is spilled on the lab bench or floor.
1. Pour disinfectant over the spill and cover with paper towels for 10 minutes.
2.Wipe up and dispose of paper towels in the biohazard bags.
3. Pour additional disinfectant over the area and wipe up with paper towels.
4. Throughly wash your hands with soap and hot water.
Biohzardous labels:
B-R-Y-W what do these letters stand for?
Blue - health hazard (inhale, skin, eyes, ingest)
Red - Flammable (organic solvents: alcohol, ether)
Yellow - Reactive-very explosive or corrode. (acids, alkali, corrosive materials)
White - Protective equipment - need goggles, shoe covers, etc..
Biohazardous labels: Rating what is 0-1-2-3-4?
0-minimal
1-slight
2-moderate
3-serious
4-extreme
What is culture medium?
Bacteria food
What makes up the culture medium?
Water-to desove the nutriants and
Broth mixed with agar to solidify.
At what temperature does the agar liquefy?
100 degrees Celsius
At what temperature does the agar solidify?
55 degrees Celsius
What is the purpose of a slant surface area in an agar slant?
Slant will increase the surface area to work with.
What is the purpose of using a petri dish?
The visibility is much better and a colony can be viewed.
A culture containing a single unadulterated species of cells is called_______________.
a pure culture
The survival and continued growth of microorganisms depend on an adequate supply of nutrients and a favorable growth environment. A solution containing these nutrients is a____________
culture medium
All culture media are_______________, _________, or__________.
Liquid, semisolid, or solid.
A liquid medium lacks a solidifying agent and is called a ________ _______.
Broth medium
A broth medium supplemented with a solidifying agent called ____________ results in a solid or semisolid medium.
agar
Organisms, especially pathogens,can be cultivated at temperatures of _______ or slightly higher without fear of the medium liquefying.
37 degrees C
What is the advantage of using a semi-soild medium?
Using a semi-soild medium has the advantage that it presents a hardened surface on which microorganisms can be grown using specialized techniques for the isolation of discrete colones.
A colony is a ________ of cells.
Cluster
Each colony is a cluster of cells that originates from the multiplication of a single cell and represents the growth of a single species of microorganism. Such a defined and well-isolated colony is a____________.
pure culture
While in liquefied state, solid media can be placed in test tubes, which are then allowed to cool and harden in a slanted position, producing________ _________.
agar slants
Agar deep tubes are used primarily for study of the gaseous requirements of microorganism. However, they may be liquefied in a boiling water bath and poured into Petri dishes, producing___________.
agar plates.
What is the advantage of using petri dishes?
provides large surface areas for the isolation and study of microorganisms.
The process of rendering a medium or material free of all forms of life.
Sterilization
After inoculation, petri dishes are incubated in an _________ position to prevent __________ that forms on the cover during solidification from dropping down onto the surface of the hardened agar.
inverted-- condensation
Name the two types of transfer agents used in this lab.
Transfer needle and transfer loop
Microorganisms must be transferred from one vessel to another or from stock cultures to various media for maintenance and study. Such a transfer is called____________.
subculturing
____________ must be carried out under sterile conditions to prevent possible contamination.
Sub-culturing
These are made from inert metals such as nichrome or platinum and are inserted into metal shafts that serve as handles.
Wire loops and needles
Wire loops and needles are extremely durable instruments and are easily sterilized by incineration in the _______ (hottest) portion of the Bunsen burner flame.
Blue
1st step in culture transfer techniques for an 'aseptic transfer' of microorganisms.
An inoculating needle or loop must always be sterilized by holding it in the hottest portion of the Bunsen burner flame, the inner blue cone, into the entire wire becomes red hot.
2nd step in culture transfer techniques for an 'aseptic transfer' of microorganisms.
The tubes are uncapped by grasping the first cap with the little finger and the second cap with the next finger and lifting the closures upward. Once removed these caps must be kept in the hand that holds the sterile inoculating loop or needle. The necks of the tubes are briefly passed through the flame.
3rd step in culture transfer techniques for an 'aseptic transfer' of microorganisms.
A loop or needle is used for removal of the inoculum.
4th step in culture transfer techniques for an 'aseptic transfer' of microorganisms.
The cell-laden loop or needle is inserted into the subculture tube. It is drawn lightly over the hardened surface in a straight or zigzag line.
5th step in culture transfer techniques for an 'aseptic transfer' of microorganisms.
the instrument is removed, the necks of the tubes are re-flamed, and the caps are replaced on the same tube from which they were removed.
6th step in culture transfer techniques for an 'aseptic transfer' of microorganisms.
The needle or loop is again flamed to destroy remaining organisms.
To add organism you want is called_____
inoculate
To add organism you do not want is called_____
contaminating
Organismisms that do no harm and they actualy protect
normal flora
These organisms that do not belong are called__________
transient flora (moving)
Transfer without the spread of organisms.
aseptic transfer