Microbiology Lab Quiz 1

Front 1

Growth Medium

Back 1

A liquid or gel designed to support the growth of microorganisms.

Front 2

Nutrient Agar Plate

Back 2

A solid medium on flat surface. This is the best method to observe colony morphology (shape) and to work with individual colonies. Best for diagnostic methods.

Front 3

Microscope

Back 3

A device used to magnify objects that are too small to be seen with the naked eye

Front 4

Specimen

Back 4

Object being looked at

Front 5

Ocular lens

Back 5

Eyepiece

Front 6

Objective lens

Back 6

Lens closest to the specimen

Front 7

Condenser

Back 7

Lens that concentrates the light for better resolution of the specimen

Front 8

Iris diaphragm

Back 8

controls the amount of light

Front 9

Rheostat

Back 9

controls intensity of light

Front 10

Course Adjustment

Back 10

used for focusing with low power objective lens

Front 11

Fine Adjustment

Back 11

used for focusing with high power and oil immersion lens

Front 12

Parfocal

Back 12

specimen will remain in focus as you change the objective lenses

Front 13

Resolution

Back 13

the ability of the lenses to reveal fine detail

Front 14

Aseptic Technique

Back 14

procedure to prevent contamination of medium or bench surface

Front 15

Inculcation

Back 15

act of transferring bacteria (and other microorganisms) onto culture medium

Front 16

Contaminant

Back 16

Unwanted microbes present in culture medium or lab bench surface

Front 17

Sterile Media

Back 17

media prepared and then sterilizedprior to use

Front 18

Gram Stain

Back 18

differential stain used to classify Gram positive and negative bacteria

Front 19

Acid Fast Stain

Back 19

differential stain that detects acid-fast and non-acid fast organisms

Front 20

Acid Fast Bacteria

Back 20

have a waxy cell wall (mycolicacid) that makes them difficult to stain with traditional methods. Also appear red/pink when stained.