Microbiology - Lab Practical #1

Front 1

where is the fire extinguisher?

Back 1

to the left of teh entrance door

Front 2

Where is the spill control station

Back 2

in the cabinet midway down the entrance door wall

Front 3

where is the eye station

Back 3

on the back wall to the right

Front 4

where is the shower

Back 4

down the hall

Front 5

what genus has a scolex, suckers, hooks and proglottids

Back 5

Taenia

Front 6

what genus has oral suckers, ovaries and testes

Back 6

Fasciola

Front 7

what genus has larvae in skeletal muscle cells and nurse cells

Back 7

trichinella

Front 8

what genus has cutting plate

Back 8

necator

Front 9

what genus has a nucleus and pseudopods

Back 9

amoeba

Front 10

what genus has cilia?

Back 10

Paramecium

Front 11

what's the feature of a microscope which allows object viewed to remain in approximate focus when objective is changed

Back 11

parfocal

Front 12

what is the vertical depth of specimen in focus

Back 12

the depth of field

Front 13

what is the area of slide being observed

Back 13

Field of view

Front 14

what genus has vegetative cells and budding cells

Back 14

candida

Front 15

how to adjust the amount of light that shines on a microscope slide

Back 15

adjust the iris diaphragm

Front 16

what are the two main structures of penicillum and aspergillus

Back 16

hyphae and conidia

Front 17

identify this elevation

Back 17

convex

Front 18

identify this elevation

Back 18

umbonate

Front 19

identify this elevation

Back 19

plateau

Front 20

identify the flat elevation

Back 20

flat

Front 21

identify the raised elevation

Back 21

raised

Front 22

identify this elevation

Back 22

raised, spreading edge

Front 23

identify this elevation

Back 23

flat, raised margin

Front 24

identify this elevation

Back 24

growth into medium

Front 25

identify the whole colony descriptor

Back 25

round

Front 26

identify the irregular whole colony descriptor

Back 26

irregular

Front 27

identify the whole colony descriptor

Back 27

filamentous

Front 28

identify the whole colony descriptor

Back 28

rhizoid

Front 29

identify the margin descriptor

Back 29

smooth, entire

Front 30

identify the rhizoid margin descriptor

Back 30

rhizoid

Front 31

identify the margin descriptor

Back 31

irregular

Front 32

identify the lobate margin descriptor

Back 32

lobate

Front 33

identify the margin descriptor

Back 33

filamentous

Front 34

describe simple stain

Back 34

only one stain is used, no decolorization ste;

Front 35

describe the order of the Gram stain

Back 35

First use Crystal violet stain (rinse with distilled water)
then fix with iodine stain (rinse with D.I.)
Then decolorize with ethanol
Then counter stain with safranin (Rinse with D.I.)

Front 36

Describe the steps for the Ziehl-Neelsen (ZN) acid-fast stain

Back 36

apply ZN carbolfuchsin stain - steam for 5 minutes (rinse w/d.i.)
decolorize with acid-alcohol (rinse w/d.i.)
counterstain with Methylene Blue then rinse with d.i.

Front 37

what differentiates candida from other fungi?

Back 37

candida does not have hyphae

Front 38

what is the liquid medium used to test for aerotolerance?

Back 38

fluid thyoglycollate medium

Front 39

if you see growth mostly at the top but also throughout a FTM sample tube, what is the aerotolerance category of the organism?

Back 39

facultative anaerobe

Front 40

if you see growth only at the very top of a FTM sample tube, what is the aerotolerance category of the organism?

Back 40

obligate aerobe

Front 41

if you see growth only at the bottom of a FTM sample tube, what is the aerotolerance category of the organism?

Back 41

obligate anaerobe

Front 42

describe aerotolerant anaerobic bacteria

Back 42

not affected by oxygen and are evenly spread out along the test tube.

Front 43

what is the genus of the org that has zygospores

Back 43

rhizopus

Front 44

what is the term for arrangement of long chains of rod-shaped bacteria

Back 44

streptobacillus

Front 45

what is the term for arrangement of long chains of sphere-shaped bacteria?

Back 45

streptococcus

Front 46

term that describes bacteria as being everywher

Back 46

ubiquity

Front 47

name the media we used for the ubiquity cultures

Back 47

sheeps blood agar

Front 48

during the Gram stain technique, the iodine stain acts a s a _________ to the crystal violet stain

Back 48

trapping agent

Front 49

for what type of bacterium is the acid-fast stain technique used

Back 49

mycobacterium

Front 50

describe why the acid-fast stain is used for mycobacterium

Back 50

b/c mycobacterium hve a waxy, hydrophobic layer of mycolic acids on the outside of the cell wall protecting it. This layer will not wash away in acid, so if treated with acid-alcohol and it doesn't wash away, you know it's an acid-fast reaction, positive for mycolic acids of mycobacterium

Front 51

===

Back 51

===

Front 52

Name the 5 stain techniques we've studied

Back 52

simple
Gram
ZN Acid-Fast
Capsule
endospore

Front 53

describe the endospore stain

Back 53

requires a 10 minute steaming period to force the Malachite Green into the endospore.

Front 54

what is the counter stain used in endospore staining

Back 54

Safranin

Front 55

what is the counter stain used in the ZN Acid Fast stain

Back 55

Methylene Blue

Front 56

what color is positive and negative for acid fast results?

Back 56

pink is positive for acid fast
blue is negative

Front 57

how deos the capsule staining technique work?

Back 57

capsules composes of polysaccharides or polyeptides that repel most stains. Acidic stain first stains the background and then a basic stain colorizes the cell proper, leaving the capsule unstained and appears as a white halo

Front 58

what are the two stains used in the Gram stain

Back 58

Crystal Violet
Safranin

Front 59

what are the two stains used in the acid fast ZN?

Back 59

Ziehl's carbolfuchsin stain
Methylene Blue

Front 60

what are the two stains used in the endospore stain?

Back 60

Malachite Green
Safranin

Front 61

what are the two stains used in the capsule stain?

Back 61

Congo red
Maneval's stain

Front 62

how do you handle a spill

Back 62

cover the spill with paper towels, soak the towels with disinfectant, allow to stand for 20 minutes. report spill to instructor. dispose of towels in biohazard barrel

Front 63

name a disease caused by an acid-fast organism

Back 63

leprosy

Front 64

name a disease that is caused by a capsulated organism

Back 64

anthrax

Front 65

name the genus of on organism that forms endospores

Back 65

Bacillus

Front 66

name a disease caused by an endospore former

Back 66

Botulism

Front 67

packets of cocci are called

Back 67

(sarcinae)

Front 68

what is the next step of transfer after you have removed the screw cap of a culture tube?

Back 68

flame the open end of the culture tube by passing it through the flame 2-3 times.