Thu Phan Khanh Nguyen Dr. Jennifer Bess MCB - 2000L - 87248 11 April 2024 Unknown Lab Report: Unknown #20 Unknown Number: #20
Bacteria identified as Enterococcus casseliflavus.
Introduction: Many bacteria have existed for hundreds of millions of years that we have not yet discovered. Those bacteria can survive under the thawing layers of ice, so when it melts, they are awakened and become strongly active. These bacteria are consumed by plants and animals, whom we also consume as our daily source of food. The basis for those bacteria can lead to certain epidemics. Therefore, the purpose of this experiment is to find out what this bacteria is and find a vaccine to fight it before it gets worse.
Materials and Methods: Choose the unknown sample from the samples that the professor has placed on the table. The first thing to do is streak the unknown samples to obtain individual colonies for additional analysis by using a sterile loop to streak the bacterial samples into TSA for incubation. Next is the Gram Staining step to determine the morphology and Gram of the species using Crystal Violet for 1 minute, Gram iodine for 1 minute, 95% ethyl alcohol for 10-30 seconds, and Safranin for 1 minute, and then when you take a sample, examine it under a microscope. Next use MSA and Blood Agar. Use a loop to streak the sample onto agar plates and incubate. Then use the SIM tube to test for sulfur reduction, indole production, and motility by using an incubation needle to transfer …show more content…
and Enterococcus strains are at the top of the list of causes of infections and hemorrhagic infections (Ventola, 2015). There are many types of Entercocci, but the two most widely known strains are Enterococcus faecalis and E. faecium, there is one strain that can also cause the disease, E. casseliflavus (Yoshino,
Bacteria identified as Enterococcus casseliflavus.
Introduction: Many bacteria have existed for hundreds of millions of years that we have not yet discovered. Those bacteria can survive under the thawing layers of ice, so when it melts, they are awakened and become strongly active. These bacteria are consumed by plants and animals, whom we also consume as our daily source of food. The basis for those bacteria can lead to certain epidemics. Therefore, the purpose of this experiment is to find out what this bacteria is and find a vaccine to fight it before it gets worse.
Materials and Methods: Choose the unknown sample from the samples that the professor has placed on the table. The first thing to do is streak the unknown samples to obtain individual colonies for additional analysis by using a sterile loop to streak the bacterial samples into TSA for incubation. Next is the Gram Staining step to determine the morphology and Gram of the species using Crystal Violet for 1 minute, Gram iodine for 1 minute, 95% ethyl alcohol for 10-30 seconds, and Safranin for 1 minute, and then when you take a sample, examine it under a microscope. Next use MSA and Blood Agar. Use a loop to streak the sample onto agar plates and incubate. Then use the SIM tube to test for sulfur reduction, indole production, and motility by using an incubation needle to transfer …show more content…
and Enterococcus strains are at the top of the list of causes of infections and hemorrhagic infections (Ventola, 2015). There are many types of Entercocci, but the two most widely known strains are Enterococcus faecalis and E. faecium, there is one strain that can also cause the disease, E. casseliflavus (Yoshino,