Materials and Methods
Materials used in experiment 1.1A: Nutrient agar plate
Materials used in experiment 1.1B: Nutrient agar plate, Sterile cotton swab In experiment 1.1B a nutrient agar plate was opened and left in an arbitrary spot in the laboratory and left untouched for three hours, at the end of the lab it was collected and incubated at . In part B a swab/swab rinse method was preformed in order to inoculate the nutrient agar plate. A sterile cotton swab was taken out of its packaging, an aseptically moistened with sterile saline. The bottom of a shoe was then swabbed. The inoculated cotton swab was then swabbed benignly onto surface of the nutrient agar. The plate was then incubated at (Ryerson Department of Biology, 2017).
Materials used in experiment 1.2A: Inoculating loop, Nutrient broth tube
Materials used in experiment 1.2B: Inoculating loop, Nutrient broth tube, Bunsen burner, Flint
In experiment 1.2B the end of an inoculating loop was brushed against one’s finger. A nutrient broth tube was then …show more content…
coli), Staphylococcus aureus (S. aureus), Bacillus subtilis (B. subtilis)), Nutrient agar plate Experiment 1.5 commenced by dividing the Nutrient agar plate into four equal segments. Aseptic technique was used to pick up a sample of broth culture using the inoculating loop. The sample was then inoculated onto the first section of the plate. The loop was flame-sterilized after the first inoculation and the plate was re-inoculated by dragging a fraction of the first inoculated sample onto the second segment of the plate. This process was repeated twice more until the entire surface of the nutrient agar plate was coated in culture. The plate was left to incubate at (Ryerson Department of Biology, 2017).
Materials used in Experiment 1.6: Escherichia coli (E. coli) culture, 5 1mL microtubes, 10 mL sterile saline, sterile 1.0 mL pipettes, pi
Materials used in experiment 1.1A: Nutrient agar plate
Materials used in experiment 1.1B: Nutrient agar plate, Sterile cotton swab In experiment 1.1B a nutrient agar plate was opened and left in an arbitrary spot in the laboratory and left untouched for three hours, at the end of the lab it was collected and incubated at . In part B a swab/swab rinse method was preformed in order to inoculate the nutrient agar plate. A sterile cotton swab was taken out of its packaging, an aseptically moistened with sterile saline. The bottom of a shoe was then swabbed. The inoculated cotton swab was then swabbed benignly onto surface of the nutrient agar. The plate was then incubated at (Ryerson Department of Biology, 2017).
Materials used in experiment 1.2A: Inoculating loop, Nutrient broth tube
Materials used in experiment 1.2B: Inoculating loop, Nutrient broth tube, Bunsen burner, Flint
In experiment 1.2B the end of an inoculating loop was brushed against one’s finger. A nutrient broth tube was then …show more content…
coli), Staphylococcus aureus (S. aureus), Bacillus subtilis (B. subtilis)), Nutrient agar plate Experiment 1.5 commenced by dividing the Nutrient agar plate into four equal segments. Aseptic technique was used to pick up a sample of broth culture using the inoculating loop. The sample was then inoculated onto the first section of the plate. The loop was flame-sterilized after the first inoculation and the plate was re-inoculated by dragging a fraction of the first inoculated sample onto the second segment of the plate. This process was repeated twice more until the entire surface of the nutrient agar plate was coated in culture. The plate was left to incubate at (Ryerson Department of Biology, 2017).
Materials used in Experiment 1.6: Escherichia coli (E. coli) culture, 5 1mL microtubes, 10 mL sterile saline, sterile 1.0 mL pipettes, pi