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25 Cards in this Set
- Front
- Back
- 3rd side (hint)
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purpose of course adjustment knob
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move stage up and down
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What is the purpose of oil immersion
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the oil on the 100x lens is to prevent the light from bending
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What is the purpose of aseptic technique
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to prevent contamination
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why do we flame the mouth of the test tube
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so no bacteria in the air contaminate the tube
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when do you flame the loop
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before and after inacculation
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why do we heat the loop
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to prevent contamination
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How long do you flame the loop
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until its red
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why do we angle the test tube
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to prevent contamination
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What type of media do we use
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slant agar
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What color is the bacteria before staining with CV
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clear lol
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before starting anything in the lab what must you do
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sanitize the table and equiptment
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What color is gram positive
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purple
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What color is gram negative
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red
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What dye is used for simple stain
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methylene blue
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What is the primary gram stain color
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purple
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What do we use as a decolorizing agent
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EtOH (alcohol)
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What do we use as a counter stain
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safranin
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What is the purpose of bibulous paper
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to blot dye and water off the slIde
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What is the purpose of lens paper
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to clean the lenses duh
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What do we use as a mordant
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iodine
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mordant =color intensifier
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What is the purpose of smearing
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to spread bacteria on the slide
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What is the purpose of fixing
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to kill bacteria and adhere it to the slide
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How do you "fix"bacteria to a slide
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pass slide over flame
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what is water used for on slide
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dilution
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how do u hold the microscope
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2 hands and close to body
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