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ATP

Adenosine triphosphate


Energy carrying molecule of the cell

How does ATP store energy?

In phosphate bonds

What happens when ATP breaks down?

It releases energy and becomes Adenosine diphosphate (ADP) in a reversible reaction

Oxidation

The loss of electrons and/or hydrogen ions

Reduction

The gain of electrons and/or hydrogen ions

What is the pneumonic for oxidation/reduction?

OIL RIG


Oxidation Is Loss


Reduction Is Gain

If something is oxidized, something else is _____

Reduced

NAD

A coenzyme that acts as a carrier molecule that is constantly reduced and oxidized

NAD cycle

NAD+ becomes NADH when it is reduced and carries those electrons and hydrogen ions to fuel other reactions

H

NAD+

Substrate

Enzyme

Product

NADH

Carbohydrate catabolism

Microorganisms oxidize carbohydrates as their primary source of energy

What is the most common energy source?

Glucose

How can energy be obtained from glucose?

1. Respiration


2. Fermentation

2

Aerobic cellular respiration

Electrons released by oxidation are passed down the Electron Transport System (ETS) with oxygen being the final electron acceptor

Aerobic cellular respiration uses the energy of one glucose molecule to power the buildup of how much ATP?

38 ATP

What is the general chemical equation for aerobic cellular respiration?

Sub-pathways of aerobic cellular respiration

1. Glycolysis


2. Transition reaction


3. Krebs cycle


4. Electron transport system

At what sub-pthway of aerobic cellular respiration does the majority of ATP get made?

Electron transport system

In bacterial cells, where does each sub-pathway of aerobic cellular respiration take place?

Glycolysis, transition reaction, and Krebs cycle occur in cytoplasm



Electron transport system takes place in cell membrane

In eukaryotes, where do the sub-pathways of aerobic cellular respiration take place?

Glycolysis takes place in the cytoplasm



Transition reaction, Krebs cycle, and electron transport system occur in the mitochondria

Glycolysis

Splitting of glucose


Oxidation of glucose into 2 molecules of pyruvic acid using Embden-Meyerhof pathway

End products of glycolysis

1. 2 molecules of pyruvic acid


2. 2 NADH


3. 2 ATP are made by substrate level phosphorylation

Preparatory stage of glycolysis

- Starting molecule is glucose


- 2 ATP are used to put phosphate groups on to the ends of the glucose molecule


- The glucose molecule is split into 2 three carbon molecules glyceraldehyde 3-phosphate (GP)

Energy- conserving stage of glycolysis

- The 2 GP molecules are oxidized and 2 NAD+ are reduced to NADJ


- In a series of enzymatic reactions, 4 ATP are produced


- End product: 2 pyruvic acid molecules

Transition reaction

- oxidation of pyruvic acid


- connects glycolysis to Krebs Cycle


- 2 molecules of pyruvic acid are further broken down into 2 molecules of acetyl coenzyme A (Acetyl CoA)

End products of transition reaction

1. 2 acetyl CoA


2. 2 NADH


3. 2 CO2 (waste product of pyruvic acid decarboxylation)

3

Krebs Cycle

- Aka citric acid or TCA cycle


- uses acetyl CoA as fuel


- series of chemical reactions that begin and end with citric acid (citrate)

End products of Krebs Cycle

1. 2 ATP


2. 6 NADH


3. 2 FADH2


4. 4 CO2

4

What happens during the Krebs Cycle?

- Acetyl CoA combines with oxaloacetic acid (4C) to make citric acid (6C)


- Citric acid is oxidized throughout the cycle, with three NADH's and one FADH2 being produced


- Every time a carbon is knocked off of the chain it is released as carbon dioxide (4 CO2 as waste)

During the Krebs Cycle, how much ATP is made by substrate level phosphorylation?

1 ATP

What happens to the oxaloacetic acid once the Krebs Cycle is complete?

It is eventually reformed so that the cycle can start anew

How many times does the Krebs Cycle run for each molecule of glucose?

Twice

Electron transport system

- Aka cytochrome system


- produces 34 ATP by oxidative phosphorylation per molecule of glucose

Where in the cell does the electron transport system take place?

Within the cell membrane in bacteria


Within the inner membrane of mitochondria in eukaryotes

Chemiosmotic Mechanisms of ATP Generation

- High energy electrons are taken off NADH and FADH2 and are passed by carriers through the membrane


- as electrons go through the membrane, they lose energy


- this energy is used to pump hydrogen ions outside the membrane


- these H ions create a highly positive charge, resulting in an electrochemical gradient


- as H+ ions rush back into the cell, they create energy to synthesize ATP

What is the final electron acceptor?

O2 inside the cell

ATP Synthase

An enzyme that synthesizes ATP

Electron Transport and the chemiosmotic mechanisms of ATP generation

- NADH and FADH are oxidized and essentially provide the fuel for this process


- high energy electrons are passed from carrier to carrier down the line


- the energy from this is used to pump hydrogen ions outside the membrane


- H ions cannot cross the plasma membrane because of their charge


- they are forced to travel through ATP synthase, which uses the energy from their passage to generate ATP

What happens to the electrons in the electron transport chain after ATP is generated?

They are eventually placed on oxygen by cytochrome oxidase. The oxygen then picked up 2 H ions to become water

For every NADH, ___ ATP is formed in ETS

3

For every FADH2, ___ ATP is formed in ETS

2