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17 Cards in this Set
- Front
- Back
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Asepsis |
Absence of organisms that may cause contamination or disease |
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Sterile |
Absence of all life |
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Mixed cultures |
When two or more bacterial species live together in the same location Seen in nature |
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Pure culture |
Container of nutrient media that contains only 1 kind of bacteria Only seen in lab |
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Why do we need bacteria in pure cultures? |
1. To identify the bacteria 2. Maintain stock cultures 3. Perform antibiotic sensitivity testing |
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Bacterial colony |
The descendants of one cell growing together on a solid surface Can be used to start a pure culture Aseptic technique is necessary to obtain this |
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Contamination |
The introduction of unwanted microorganisms in a particular environment |
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Aseptic/sterile techniques function to: |
1. Protect the culture 2. Protect you and the environment |
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Inoculating loop |
A tool composed of inert metal (such as platinum) that is used transferring bacteria from a liquid broth media |
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Inoculating needle |
Lab tool composed of inert metal (such as platinum) used when transferring bacteria from a solid agar media |
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Forceps |
Lab tool sterilized by alcohol flaming |
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Pipettes |
Sterile glass tubes that can deliver a set amount of liquid
Used in combination with a pipette pump |
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How is contamination avoided? |
1. Direct flaming 2. Alcohol flaming |
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Direct flaming |
Process in which heat is applied to an inoculating loop or needle in order to kill bacterial cells |
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What is the proper way to heat inoculating loops and needles? |
To avoid creating bacterial aerosols, heat needles and loops from handle to tip |
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Alcohol flaming |
Process that involves dipping forceps into a beaker of alcohol and then passing them through a flame until the alcohol has burned off. This functions to sterilize the tips of forceps |
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Turbidity |
Cloudiness Indicates microbial growth |
