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33 Cards in this Set

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What is the proper way to carry a microscope?

One hand holding the arm, the other hand supporting base

What can be used up clean the ocular and objective lens?

Lens paper

How does one bring different objectives into place?

Rotate the nosepiece

How does one center the slide over the light hole?

Turning the mechanical stage adjustment knobs

What parts of the microscope can be used to adjust light intensity?

1. Diaphragm lever


2. Rheostat knob

2

What is used on the slide you wish to observe in 100X objection?

Immersion oil

What happens to the light intensity as magnification increases?

It must be increased

What objectives can be used once oil has been added to a slide?

100X


10X


4X

Formula for total magnification

Objective Magnification x Ocular Magnification

Total magnification of scanning objective

4x10= 40

Total magnification of low power objective

10x10= 100

Total magnification of high power objective

40×10= 400

Total magnification of oil immersion objective

100×10= 1000

Resolving power

Aka resolution


The microscope's ability to distinguish close together objects and fine detail

How is resolution of light microscopes improved? Who discovered this?

By adding more light


Ernest Abbe

What is the purpose of immersion oil?

It prevents the loss of light rays due to its refractive index (same as glass)

Parfocal

What most of our microscopes are.



The lenses are adjusted so that when you are looking at a specimen in one objective, it should remain mostly in focus when you switch to another objective

When you move the slide to the right, what happens? Why is this?

The image moves to the left


Due to the setup of the lenses, images appear inverted

Depth of field

One must focus up and down through the slide to find what one is looking for when dealing with a thick specimen

Power switch

Rheostat

Course focus arrangement knob

Fine focus adjustment knob

Mechanical stage control

Base

Light source

Condenser

Diaphragm lever

Stage

Slide holder

Oil immersion objective lens

Nosepiece

Ocular lens