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9 Cards in this Set
- Front
- Back
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Why is it important to achieve the correct dose of drug in patients |
Gain maximum therapeutic benefit Prevent unwanted side effectshiw |
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How do we measure the drug in patient |
Measure the drug in serum by direct method( HPLC) Measure the drug in serum by immunoassay(FPIA) |
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What does Fluorescent polarisation immunoassay do |
Polarised light is used to excite a fluorescent label on a drug |
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What does fluorescent polarisation immunoassay require |
Fluorescently labeled drug Antibody to drug Machine |
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Explain FPIA DRUG with a fluorescent label |
Light causes the free drug to spin. Polarisation is lost in emitted light |
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FPIA drug with fluorescent label antibodies that bind the drug |
As the drug is bound by ab its prevented from spinning Polarisation is kept |
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FPIA on drug with fluorescent label antibodies that bind drug samples that contains a high drug Concentration |
The non labelled drug binds Ab leaving labeled ab free to rotate so polarisation is lost |
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FPIA drug with fluorescent label antibodies that bind drug sample containing low drug concentration |
Non sufficient unlabelled drug to bind Ab therefore labeled drug also bound by Ab doesnt rotate and polarisation is conserved |
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What is FPIA responsible for and what does it measure |
Responsible for the success of therapeutic drug monitoring Can measure drug concentrations in serum accurately quickly and easily |
