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11 Cards in this Set
- Front
- Back
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What is anaphylactic shock |
Systemic response - increased vascular permeability -swelling of lips tounge -smooth muscle contraction of lungs -fall in bp -death is a possibility |
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What are the benefits of allergy testing |
Identify allergens for a person so they can be avoided May be especially important in industrial setting Desensitisation treatment |
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Explain passive cutaneous anaphylaxis |
Inject rats with evans blue Inject rats with serum sample from patient Antibodies from serum diffuse into tissue ige will bind to fc receptors on mast cell) Test allergen is injected intradermally Allergen would be bound by mast associated IgE IGe cross linking occurs Mast cell degranulation Increased vascular permeability Evans blue escapes into skin obvious wheal and flare infection |
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What does radioimmunosorbant (RIST) assay measure |
For total IgE |
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Name how rist works |
Disk with anti ige is bound to surface Add the serum sample Add radio labelled anti-IgE Quantify the reaction |
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Name the steps in a RAST assay |
Disk with allergen bound to surface Add serum sample Add radiolabeled anti-IgE Quantify radiation |
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Differences between RIST AND RAST |
RAST-can determine which allergen a person responds RIST- can only determine if a person has elevated IgE |
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Whats some future protein arrays |
Miniaturization and testing of hundreds of different antigens on a microarray Replacement of radiolabel by fluorescent label |
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What do anti histamines do |
Block release or action of histamines |
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Increasing doses of antigen injected subcutaneously results in what |
A shift from IgE to IGG production |
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Bead array adv |
Can mix and match beads to make custom arrays for individual patients |
