Protozoology Lab Exam Final

Front 1

Identify this and the structures

Back 1

Giardia cyst

Front 2

Identify this and structures

Back 2

Hint 2

Giardia Cyst, notice the structures

Front 3

What are these and where would you find them?

Back 3

Hint 3

Giardia cysts

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Back 4

Giardia trophozoite.

Front 5

What are these?

Back 5

giardia trophozoite

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Back 6

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Back 7

giardia cysts

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Various stages of E. histolytica cysts. A) An early precyst with a single nucleus. B and D) A cyst at the 2 nucleus stage. C) A mature cyst with 4 nuclei and no chromatoid bodies. Note how the nuclei decrease in size with each subsequent division. A-C are stained with trichrome and D is stained with iron hematoxylin.

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Back 9

crescent-shaped gametocytes
P falciparum

Front 10

Which plasmodium has a crescent shaped gametocyte?

Back 10

P. Falciparum

Front 11

What is this?

Back 11

P falciparum ring stage. These are early trophozoitesmay appear to have two nuclei

Front 12

Which Plasmodium have Schüffner’s dots?

Back 12

vivax and ovale

Front 13

What is the halo around the falciparum gametocyte known as?

Back 13

Laveran's bib

Front 14

Plasmodium vivax

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may have irregular ameboid appearance
reddish granules called Schueffner granules

Front 15

What are Plasmodium parasites with 2 or more nuclei known as?

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schizont

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vivax schizont

Front 17

what is this?

Back 17

Ring-form of P. vivax have a thick cytoplasm with a single, large chromatin dot. The cytoplasm becomes amoeboid, Schüffner's dots appear as the trophozoites mature. Infected RBCs are often larger

Front 18

What are the two types of falciparum in circulation?

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ring stage and gametocytes

Front 19

What is a distinguishing feature of vivax trophozoit and schizont infection of eurythrocytes?

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Schueffner's dots will be reddish

Front 20

What are plasmodium parasites with more thatn one nucleus called?

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schizont

Front 21

trophozoite of P malariae

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compact and deeply staining, no enlargement of the host cell

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Trophozoite of vivax

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ameboid and host cell is enlarged. Schnueffer's dots

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Schizont of malariae

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rosette structure. 8-12 nuclei

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Schizont of vivax

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enlarged and has Schnueffer's dotscan contain up to 32 nuclei/merozoites

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Gametocyte of malariae

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fills entire normal sized erythrocyte

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Gametocyte of vivax

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nearly fills entire enlarged host and Schnuffer's dots present

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Composite showing various morphological forms of P. malariae. ri = ring; tr = trophozoites; sz = schizont; sg = segmenter; gm= gametocyte; p = platelets

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Schizonts of P. ovale in thin blood smears

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Composite showing various morphological forms of P. malariae. ri = ring; tr = trophozoites; sz = schizont; sg = segmenter; gm= gametocyte; p = platelets

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Composite showing various morphological forms of P. vivax. ri = ring; et = early trophozoite; lt = late trophozoite; sz = schizont; gm= gametocyte; p = platelets

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malariae schizonts

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are usually compact not enlaged

Front 33

distinguish between gametocytes of ovale and vivax

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vivax larger.

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Back 34

Schizonts of P. ovale tend to be smaller and contain fewer merozoites (average 8). Elongation to an oval shape Schüffner's dots can be observed with proper staining.

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vivax schizont

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ovale schizont

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Back 36

ovale rings

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T. cruzi

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African Trypanosome

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Back 38

Front 39

Back 39

trypomastigote of African Trypanosome spp

Front 40

which Trypanosome spp has a trypmastigote dividing cycle?

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African Trypanosome. T. Cruzi does not. In T cruzi, this is the epimastigote form

Front 41

trypomastigote: kinetoplast at anterior end. nucleus in middle

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Epimastigote: kinetoplast in middle next to nucleus. This is the replicating form of T cruzi. has shorter um

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amastigote

Front 44

where are the trypomastigotes of the African trypanosomes found?

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in the blood. this is the replicating form. in the lab these are all obtained from rats or mice

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trypomastigotes of African Trypanosome. notice the replicating form

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T. gambiense and T. cruzi blood-stage trypomastigotes. notice that the Tg has a more prominate um.
The kinetopplastid seems to be larger in diameter than body or T cruzi also more often c shaped

Front 47

What forms of T cruzi are in humans

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Trypomastigote and amastigote. Amastigote is intracellular and replicate in cell

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T cruzi amastigotes in tissue. Dont forget to identify the interior kinetoplast

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T cruzi in tissue. Notice the nuclei and kinetoplast

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In lab, where is the epimastigote of T cruzi from?

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culture smear

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typical epimastigpte T cruzi. found in vector. replicating form. kintoplast appears as bar

Front 52

In lab what are the specimens from bone marrow and liver?

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amastigotes of Leishmania

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Are the amastigotes of T cruzi and Leishmania distinguishable?

Back 53

No, must know what kind of tissue and cells. Leishmania only infect phagocytes

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Leishmania promastigotes from culture. not found in human tissue

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distinguishing T cruzi epimastigotes from Leishmania promatigote

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Promastigote does not have um and are fatter also not found in human tissue

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T cruzi trypomastigote

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T cruzi epimastigote

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P. malariae ring

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ovale rings

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ovale trophozoite

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ovale trophozoite. notice the fringe