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125 Cards in this Set
- Front
- Back
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Kinyoun Acid fast purpose |
Detects Acid fast mycrobacteria |
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Kinyoun Acid fast principle |
Resistant to decolorization Will take in carbon fuchsin stain |
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Kinyoun Acid fast reagents |
95% alcohol - hydrate Carbol fuchsin solution -Stains lipoid capsule Acid (HCl) alcohol - differentiation Methylene blue - counterstain |
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Kinyoun Acid fast results |
Acid - fast organisms : Red Background : light blue |
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Front (Term) |
Kinyoun Acid fast stain |
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Kinyoun Acid fast fixative |
10% NBF (NO CARNOY - false negative) |
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Kinyoun Acid fast fixative |
10% NBF (NO CARNOY - false negative) |
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Kinyoun Acid fast cut |
4-5 microns |
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Kinyoun Acid fast troubleshooting |
NO TAP WATER Easy to over counterstain Wash acid prior to counterstain NOT satisfactory for m. Leprae |
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Kinyoun Acid fast fixative |
10% NBF (NO CARNOY - false negative) |
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Kinyoun Acid fast cut |
4-5 microns |
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Kinyoun Acid fast troubleshooting |
NO TAP WATER Easy to over counterstain Wash acid prior to counterstain NOT satisfactory for m. Leprae |
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Ziehl-Neelsen is a similar modification of what stain? |
Kinyoun |
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Kinyoun Acid fast fixative |
10% NBF (NO CARNOY - false negative) |
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Kinyoun Acid fast cut |
4-5 microns |
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Kinyoun Acid fast troubleshooting |
NO TAP WATER Easy to over counterstain Wash acid prior to counterstain NOT satisfactory for m. Leprae |
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Ziehl-Neelsen is a similar modification of what stain? |
Kinyoun |
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Ziehl-Neelsen reagents |
0.5 g basic fuchsin 2.5 ml phenol 5 ml alcohol 700 ml water |
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Ziehl-Neelsen microwave modification solution |
0.8 g basic fuchsin 7.5 ml phenol 14 ml isopropyl 140 ml water |
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Kinyoun Acid fast fixative |
10% NBF (NO CARNOY - false negative) |
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Kinyoun Acid fast cut |
4-5 microns |
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Kinyoun Acid fast troubleshooting |
NO TAP WATER Easy to over counterstain Wash acid prior to counterstain NOT satisfactory for m. Leprae |
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Ziehl-Neelsen is a similar modification of what stain? |
Kinyoun |
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Ziehl-Neelsen reagents |
0.5 g basic fuchsin 2.5 ml phenol 5 ml alcohol 700 ml water |
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Ziehl-Neelsen microwave modification carbol fuchsin solution |
0.8 g basic fuchsin 7.5 ml phenol 14 ml isopropyl 140 ml water |
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Ziehl-Neelsen microwave modification reagents |
Carbol fuchsin solution 0.5% acid alcohol solution Methelyene blue counterstain |
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Fite method purpose |
Detect mycobacterium leprae |
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Fite method reagents |
Xylene peanut oil - depar Carbol fuchsin - lipoid stain Acid alcohol - differentiation Methylene blue - counterstain |
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Why should you use xylene peanut oil when using fite method? |
Prevents shrinkage and injury Protects waxy capsule of M. Leprae |
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Fite method results |
M. Leprae and other AF orgs - Red Background - light blue |
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Front (Term) |
Fite method - m. Leprae |
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Fite method results |
M. Leprae and other AF orgs - Red Background - light blue |
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Front (Term) |
Fite method - m. Leprae |
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Fite method control |
Leprosy organism |
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Fite method cut |
4-5 microns |
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Fite method fixative |
10% NBF (NO CARNOY) |
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Auramine-Rhodamine purpose |
Detects Acid fast organisms (m. Tuberculosis) |
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Auramine-Rhodamine principle |
Unknown |
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Auramine-Rhodamine reagents |
Auramine O-Rhodamine B solution Acid alcohol - differentiation Eriochrome black T - counterstain |
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Auramine-Rhodamine results |
Acid fast - reddish yellow fluorescent Background - Black |
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Auramine-Rhodamine control |
Positive & negative control |
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Auramine-Rhodamine fixative |
10% NBF |
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Auramine-Rhodamine cut |
4-5 microns |
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Brown-hopps modification purpose |
Gram stain |
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Brown-hopps modification reagent |
Crystal violet Grams iodine Acetone - decolorizer Basic fuchsin - counterstain Gallegos solution - differentiation Picnic acid aceton - background stain / diff |
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Brown-hopps modification results |
Gram positive - blue black Gram negative - Red Background - yellow Nuclei - light red |
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Front (Term) |
Brown-hopps modification |
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Brown-hopps modification control |
Gram (+ and -) |
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Brown-hopps modification fixative |
10% NBF |
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Brown-hopps modification cut |
4-5 microns |
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Brown-hopps modification common problems |
(-)gram orgs stain purple = not enough time in acetone (-) gram not appearing = more time in gallego, too much time in acetone (+) gram not staining correct=excessive time in acetone, dead bacteria |
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May-Grunwald Giemsa stain purpose |
Demonstrate microorganisms Differentiate hematopoietic tissue cells |
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May-Grunwald Giemsa stain principle |
Romanowsky stain Polychromatic |
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May-Grunwald Giemsa stain reagents |
Lugol iodine/sodium thiosulfate- remove mercury pigment Methanol - fixative Jenner sol - eosin/methylene blue Giemsa sol - Azure B/Eosin Y Acetic acid - differentiation |
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May-Grunwald Giemsa stain reuslts |
Nuclei - blue WBC cytoplasm - shades of pink, gray or blue Bacteria - blue |
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May-Grunwald Giemsa stain control |
Spleen |
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May-Grunwald Giemsa stain fixative |
Zenker or B-5 10% NBF okay |
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May-Grunwald Giemsa stain reuslts |
Nuclei - blue WBC cytoplasm - shades of pink, gray or blue Bacteria - blue |
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May-Grunwald Giemsa stain control |
Spleen |
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May-Grunwald Giemsa stain fixative |
Zenker or B-5 10% NBF okay |
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May-Grunwald Giemsa stain cut |
3-4 microns |
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Modified Diff-Quik Giemsa purpose |
Demonstrate H. Pylori (Gastritis, peptic ulcers, gastric carcinoma and lymphoma) |
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Modified Diff-Quik Giemsa reagents |
Solution I - Eosin Y buffered Solution II - mixture of Azure A & methylene blue Acetic acid water - differentiation |
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Modified Diff-Quik Giemsa reagents |
Solution I - Eosin Y buffered Solution II - mixture of Azure A & methylene blue Acetic acid water - differentiation |
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Modified Diff-Quik Giemsa results |
H. Pylori - dark blue Other bacteria - blue Nuclei- dark blue Cytoplasm - pink |
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Modified Diff-Quik Giemsa control |
H. Pylori section |
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Modified Diff-Quik Giemsa fixative |
10% NBF |
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Modified Diff-Quik Giemsa cut |
4-5 microns |
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Modified Diff-Quik Giemsa troubleshooting |
Dehydrate slides quickly to reduce excess decolonization |
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Alcian Yellow-Toluidine blue purpose |
H. Pylori detection |
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Alcian Yellow-Toluidine blue results |
Mucin - yellow H. Pylori- blue |
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PAS - Fungal purpose |
Demonstrate fungi |
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PAS - Fungal reagents |
Periodic Acid - oxidizer Schiff reagent - aldehyde reactor Sulfurous acid solution - wash Tap water - developer Fast green- counterstain |
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PAS - Fungal results |
Fungi- rose Background - Green |
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PAS - Fungal control |
Fungi tissue |
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PAS - Fungal results |
Fungi- rose Background - Green |
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PAS - Fungal control |
Fungi tissue |
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PAS - Fungal fixative |
10% NBF, bouins, Zenkers |
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PAS - Fungal cut |
4-5 microns |
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PAS - Fungal technical notes |
Use fats green as counterstain Use fresh reagents for proper staining |
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(CAS) Chromic Acid Schiff is the same technique as |
PAS |
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CAS) Chromic Acid Schiff uses what oxidizer instead of periodic acid? |
Chromic Acid |
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Front (Term) |
CAS) Chromic Acid Schiff |
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Gridley stain purpose |
Fungi demonstration |
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Gridley stain reagent |
Chromic Acid - oxidizer Schiff reagent - aldehyde reaction Aldehyde fuchsin - occupies uninvolved schiff linkage Metanil yellow |
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Gridley stain results |
Fungi - deep rose to deep purple Background - yellow Elastic fibers and mucin - deep purple |
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Gridley stain control |
Fungi |
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Gridley stain fixative |
10% NBF |
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Gridley stain cut |
4-5 microns |
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Grocott/Gomori Methenamine silver (GMS) purpose |
Demonstrate fungi |
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Grocott/Gomori Methenamine silver (GMS) reagents |
Chromic Acid - oxidizer fungal cell wall Sodium bisulfate - removes chromic Acid Warm Methenamine silver sol - impregnate Gold chloride- toning Sodium thiosulfate- remove unbound silver Light green - counterstain |
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Grocott/Gomori Methenamine silver (GMS) results |
Fungi - Black Mucin - taupe / dark gray Background - green |
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Grocott/Gomori Methenamine silver (GMS) control |
Fungi |
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Grocott/Gomori Methenamine silver (GMS) fixative |
10% NBF (avoid gluteraldehyde) |
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Grocott/Gomori Methenamine silver (GMS) cut |
4-5 microns |
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Front (Term) |
Grocott/Gomori Methenamine silver (GMS) |
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Front (Term) |
Overstained Grocott/Gomori Methenamine silver (GMS) |
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Grocott/Gomori Methenamine silver (GMS) special technique |
Silver technique- non mentallic forceps and clean glass wear |
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What other stains can stain cryptococcus neoformans |
Alcian blue pH 2.5 Mayer mucicarmine |
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Warthin-Starry purpose |
Demonstrate spirochetes |
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Warthin-Starry principle |
Argyrophilic |
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Warthin-Starry principle |
Argyrophilic |
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Dieterle stain results |
Bacteria - brown to black Background - pale yellow/tan |
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Dieterle stain control |
Spirochetes or legionella tissue |
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Dieterle stain fixative |
10% NBF |
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Dieterle stain cut |
4-5 microns |
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Dieterle stain cautions: |
Beware of toxic chemical: uranyl nitrate & pyridine |
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Front (Term) |
Warthin-Starry |
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Warthin-Starry reagents |
Hydrate tissue to acid water Silver nitrate - impregnate Developer sol - silver nitrate & hydroquinone & gelatin |
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Warthin-Starry results |
Spirochetes - Black Other bacteria - Black Background - yellow |
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Warthin-Starry control |
Tissue containing spirochetes |
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Warthin-Starry fixative |
10% NBF |
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Warthin-Starry cut |
4-5 microns |
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Front (Term) |
Warthin-Starry |
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Dieterle stain purpose |
Demonstrate spirochetes and legionella organisms |
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Dieterle stain principle |
Argyrophilic |
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Front (Term) |
Dieterle stain |
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Steiner & Steiner purpose |
Demonstrate Spirochetes, h. Pylori, and legionella |
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Steiner & Steiner principle |
Argyrophilic |
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Steiner & Steiner reagents |
Uranyl nitrate - sensitizer Silver nitrate - impregnation Gum mastic - unknown Reducing sol - hydroquinone, gum mastic, alcohol |
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Steiner & Steiner results |
Bacteria - dark brown to black Background - light yellow |
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Steiner & Steiner control |
Tissue containing spirochetes, h. pylori, or L. Pneumophila |
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Steiner & Steiner fixative |
10% NBF |
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Steiner & Steiner cut |
4-5 microns |
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Front (Term) |
Steiner & Steiner |
