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69 Cards in this Set
- Front
- Back
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Cresyl Echt Violet purpose |
Demonstrate nissl substance Chromatolysis (injures axons) |
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Cresyl Echt Violet principle |
RNA content in nissl subs. = basophilic |
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Cresyl Echt Violet control |
Spinal chord |
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Cresyl Echt Violet cut |
6-8 micr |
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Cresyl Echt Violet fixative |
10% NBF |
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Cresyl Echt Violet method 1 staining techniques |
-Regressive stain Cresyl echt violet (aniline dye) Alcohol (differentiations) Balsam-Xylene (differentiations) |
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Cresyl Echt Violet method 1 results |
Nissl & nuclei - blue/purple Background - colorless |
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Cresyl Echt Violet method 2 |
Vacca method |
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Cresyl Echt Violet method 1 |
Luna Method |
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Cresyl Echt Violet method 2 technique |
Progressive stain Cresyl Echt Violet - aniline dye Acetic acid - acidified stain (ph2.5) |
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Cresyl Echt Violet method 2 results |
Nissl & nuclei - blue/purple Background - colorless |
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Front (Term) |
Luna Cresyl Echt Violet method 1 |
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Front (Term) |
Vacca Cresyl Echt Violet method 2 |
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Bodian Silver method purpose |
Nerve fibers Wallerian degeneration |
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Bodian Silver method reagents |
Protargol S Cooper Hydroquinone Gold chloride Oxalic acid Sodium thiosulfate Aniline blue (optional) |
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Bodian Silver method results |
Nerve fibers & nuclei - Black Background - light blue / gray |
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Bodian Silver method control |
Cerebral cortex or peripheral nerve (NO SPINAL CORD) |
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Bodian Silver method cut |
6-8 mic |
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Bodian Silver method fixative |
10% NBF |
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Holmes silver nitrate method purpose |
Nerve fibers |
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Holmes silver nitrate method reagents |
Impregnating solution Reducing solution Gold solution Oxalic Acid Sodium thiosulfate Counterstain |
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Holmes silver nitrate method results |
Nerve fibers - Black |
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Holmes silver nitrate method control |
Cerebral cortex |
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Holmes silver nitrate method cut |
10-15 mic |
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Holmes silver nitrate method fixative |
10% NBF |
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Bielschowsky - PAS stain purpose |
Nerve fibers Neurofibrillary tangles & senior plaques (alzheimer) |
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Bielschowsky - PAS stain reagents |
Ammoniacal silver Developing solution Gold chloride Sodium thiosulfate Periodic Acid Schiff reagent |
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Bielschowsky - PAS stain results |
Tangled/plaques - dark black Axons - Black Amyloid - magenta Lipofuchsin - magenta |
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Bielschowsky - PAS stain control |
CNS |
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Bielschowsky - PAS stain cut |
8-10 micro |
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Bielschowsky - PAS stain fixative |
10% NBF |
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Front (Term) |
Bielschowsky - PAS stain |
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Bielschowsky - microwave modification results |
Tangles/plaques - dark brown/black Axons - brown /black Lipofuchsin - brown or black Neuromelanin - Black |
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Front (Term) |
Bielschowsky - microwave modification |
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Sevier-munger modification of bielschowsky results |
Tangles/plaques - Black Nerve ending/fibrils - Black |
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Sevier-munger modification of bielschowsky control |
CNS |
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Sevier-munger modification of bielschowsky cut |
6-8 microns |
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Sevier-munger modification of bielschowsky fixative |
10% NBF |
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Front (Term) |
Sevier-munger modification of bielschowsky |
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Thioflavin S purpose |
Neurofibrillary tangles & senior plaques (Alzheimer’s) |
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Mallory PTAH results |
Glial fibers, myelin, nuclei - blue Neurons - salmon |
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Front (Term) |
Mallory PTAH |
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Mallory PTAH control |
Cerebral cortex |
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Mallory PTAH cut |
6-8 microns |
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Mallory PTAH fixative |
10% NBF |
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Thioflavin S reagents |
Pretreat: potassium permanganate, potassium metabisulfate & oxalic Acid, sodium hydroxide & hydrogen peroxide Thioflavin S (stains amyloid plaques) |
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Thioflavin S results |
Tangles/plaques - bright green |
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Thioflavin S control |
CNS |
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Thioflavin S cut |
6 microns |
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Thioflavin S fixative |
10% NBF |
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Front (Term) |
Mallory PTAH |
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Tungsten - hematein stains glial fibers what color? |
Blue |
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Unbound phototungstic acid stains neurons what color? |
Red-brown |
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Mallory PTAH reagents |
Zenkers Lugol iodine Alcohol Potassium Oxalic Acid PTAH soln |
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Mallory PTAH was replaced by what? |
IHC |
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Holzer method purpose |
Demonstrate glial fibers |
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Holzer method reagents |
Phosphomolybdic Acid Alcohol-chloroform Crystal violet Potassium bromide Differentiate soln |
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Glial fibers are stained with what? |
Crystal violet |
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Holzer method results |
Glial fibers - blue/purple Background - pale blue / colorless |
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Holzer method control |
Cerebral cortex |
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Holzer method cut |
6-8 microns |
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Holzer method fixative |
10% NBF |
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Use safety precautions when using Holzer method due to the: |
Aniline oil & chloroform |
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Cajal stain purpose |
Demonstrate astrocytes |
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Cajal stain reagents |
Formalin ammonium bromide Gold sublimate Sodium thiosulfate |
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Cajal stain results |
Astrocytes - black |
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Cajal stain control |
Cerebral cortex (NO SPINAL CHORD) |
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Cajal stain cut |
20-30 microns frozen sections (free floating) |
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Front (Term) |
Cajal stain |
