Micro Lab

Front 1

identify

Back 1

mycelium (lactophenol cotton blue stain )

Hint 1

mold

Front 2

identify the mold

Back 2

Sabouraud agar plates

Hint 2

mold

Front 3

what do these plates depict?

Back 3

confirmed test from streak plate technique positive for coliform,the purple are pure cultures while the others re mixed cultures (E.coli,green & Enterobacter aerogenes,purple)

Hint 3

coliform test 2nd stage

Front 4

what are these?

Back 4

soil sample results showing bacteria colonies CFU

Hint 4

serial dilution of soil

Front 5

explain d picture

Back 5

Halobacterium salinarium #21 had highest NaCl concentration at 25%(archaea ).Next is staphylococcus epidermidis #41 on our skin,had d widest range of NaCl tolerance from low to moderate.E.coli is non halophile

Hint 5

optimum growth of diff bacteria

Front 6

what test is this?

Back 6

testing to see if d chemicals killed d vegetative cells not d endospores. Some resistant cells still grew on d zone of inhibitions.

Hint 6

control of growth lab using disinfectants & antiseptics

Front 7

what lab is this?

Back 7

the controls were covered with sterile foil & put in UV light & incubated for 48 hrs at 37'c.In d results, UV light killed vegetative cells on the side that wasn't covered,growth was inhibited.While our control that was covered by foil had growth.However, endospores became vegetative cells later on d uncovered part.

Hint 7

UV light kills bacteria by creating thymine dimers dat distorts bacteria DNA causing problems in replication & transcription

Front 8

only plate at 4X with a plate

Back 8

physarum polycephalum(plasmodial slime mold)with scleratium (dormant )at the middle & strings of plasmodium.

Hint 8

cytoplasmic streaming carrying nutrients around

Front 9

what's this?

Back 9

rhizopus mold.In asexual reproduction, the spore producing body is known as sporangium & it produces spores called sporangiospores

Hint 9

rhizopus stolonifer( bread mold)

Front 10

what stain is this?

Back 10

acid fast stain or Ziehl-Neelson(stains cell due to mycolic acid). Acid fast positive stains pink/red mycobacterium smegmatis while other non acid fast,staphlococcus epidermidis stains blue (due to counterstain methylene blue)mixed culture.

Hint 10

differential stain

Front 11

identify this stain?

Back 11

crystal violet method(negative stain for endospore stain. Endospores remain colorless while vegetative cells stain purple.The organism is gram positive rod bacillus subtilis.

Hint 11

special stain(crystal violet is a purple )

Front 12

for the effect of osmotic pressure lab

Back 12

we obtained each of d nutrient agar plates & one halophile agar plate & labeled them with the organism to be tested & our group symbol. Then we used sterile technique to streak a loopful of each culture across d plate. Then we incubated 25% NaCl plates in d humid chamber for 7 days at 37' C& incubated all other plates in a rack for 48 hrs at 37'C.

Front 13

Day 2 of osmotic pressure lab

Back 13

we compared thickness of d growth lines for each organism across the 5 plates. Halobacterium salinarium had its optimal growth at d highest NaCl concentration at 25%

Front 14

what happened as we increased radiation?

Back 14

growth went down (decreased )

Front 15

names of disinfectants & antiseptics used in control of growth lab are

Back 15

Lysol & bleach (disinfectants ) & antiseptics includes Isopropyl Alcohol & Hydrogen peroxide

Front 16

The reults of day 2 of control of growth using antiseptics & disinfectants

Back 16

Observe the bottom of each plate,looking at the area of the culture around each filter paper disk for a clear zone of inhibition measure & record d diameter in mm.In our result above,notice some colonies growing within the zone of inhibition,which means they're probably resistant to d chemicals

Hint 16

anticeptics used were hydrogen peroxide & isopropyl alcohol & disinfectants used were lysol & bleach

Front 17

yeast we used in lab

Back 17

Saccharomyces cerevisiae aka bakers yeast

Front 18

define coliform

Back 18

All facultative anaerobe,gram negative rod that produce acid & gas from lactose fermentation in 48 hrs at 35'C

Hint 18

indicator organism

Front 19

slime mold used in lab

Back 19

physarum polycephalum

Hint 19

many heads

Front 20

How were mold samples collected?

Back 20

agar plates were left open for 5mins outdoors and for 20 mins indoors to collect spores

Front 21

An MPN (Most Probable Number) greater than 0

Back 21

presumes d presence of coliforms & renders water unportable

Hint 21

portable water must ve an MPN of 0

Front 22

stain used to view mold structures was

Back 22

lactophenol cotton blue stain

Front 23

selective medium for molds was

Back 23

Sabouraud agar plates

Hint 23

has low ph & high sugar hence it's selective for molds & inhibits bacteria

Front 24

medium for slime mold was

Back 24

tap water agar,to rehydrate sclerotium.Oats were used for feeding

Front 25

molds & yeasts belong to the

Back 25

Dimain Eukarya & the kingdom fungi

Front 26

penicillin,aspergillus,rhizopus & coccidiodes

Back 26

are natural soil fungi but can cause opportunistic diseases known as mycoses when a host is immunocompromised

Hint 26

mycoses

Front 27

plasmodium & sclerotium

Back 27

plasmodium is d yellow living metabolizing form at center while sclerotium is d dormant dry form

Hint 27

slime mold physarum polycephalum

Front 28

halophiles re categorized based on salt tolerances as

Back 28

facultative halophile (staphlococcus epidermidis lives on our skin & had d widest range of tolerance )& obligate halophile(Halobacterium salinarium )

Front 29

why is green sheen produced by E.coli?

Back 29

becos of d acid,when it ferments,it makes d acid

Front 30

3 steps of water testing

Back 30

presumptive test (used dilutions & pipetted out water into tubes,observed yellow color for positive),confirmed test (used streak plate tech to culture bacteria onto Eosin Methylene Blue(EMB) agar,incubated it,then identify if it's Enterobacter aerogenes(purple colonies) or E.coli (green metallic sheets).Completed test (final stage)

Front 31

synergism

Back 31

A confirmed test is necessary due to acid & gas produced separately by two or mixed bacteria in a tube hence giving false positive result.

Hint 31

positive coliform result must have both acid & gas(significant amount of bubble) present in the tube.