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31 Cards in this Set

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mycelium (lactophenol cotton blue stain )
identify the mold
Sabouraud agar plates
what do these plates depict?
confirmed test from streak plate technique positive for coliform,the purple are pure cultures while the others re mixed cultures (E.coli,green & Enterobacter aerogenes,purple)
coliform test 2nd stage
what are these?
soil sample results showing bacteria colonies CFU
serial dilution of soil
explain d picture
Halobacterium salinarium #21 had highest NaCl concentration at 25%(archaea ).Next is staphylococcus epidermidis #41 on our skin,had d widest range of NaCl tolerance from low to moderate.E.coli is non halophile
optimum growth of diff bacteria
what test is this?
testing to see if d chemicals killed d vegetative cells not d endospores. Some resistant cells still grew on d zone of inhibitions.
control of growth lab using disinfectants & antiseptics
what lab is this?
the controls were covered with sterile foil & put in UV light & incubated for 48 hrs at 37'c.In d results, UV light killed vegetative cells on the side that wasn't covered,growth was inhibited.While our control that was covered by foil had growth.However, endospores became vegetative cells later on d uncovered part.
UV light kills bacteria by creating thymine dimers dat distorts bacteria DNA causing problems in replication & transcription
only plate at 4X with a plate
physarum polycephalum(plasmodial slime mold)with scleratium (dormant )at the middle & strings of plasmodium.
cytoplasmic streaming carrying nutrients around
what's this?
rhizopus mold.In asexual reproduction, the spore producing body is known as sporangium & it produces spores called sporangiospores
rhizopus stolonifer( bread mold)
what stain is this?
acid fast stain or Ziehl-Neelson(stains cell due to mycolic acid). Acid fast positive stains pink/red mycobacterium smegmatis while other non acid fast,staphlococcus epidermidis stains blue (due to counterstain methylene blue)mixed culture.
differential stain
identify this stain?
crystal violet method(negative stain for endospore stain. Endospores remain colorless while vegetative cells stain purple.The organism is gram positive rod bacillus subtilis.
special stain(crystal violet is a purple )
for the effect of osmotic pressure lab
we obtained each of d nutrient agar plates & one halophile agar plate & labeled them with the organism to be tested & our group symbol. Then we used sterile technique to streak a loopful of each culture across d plate. Then we incubated 25% NaCl plates in d humid chamber for 7 days at 37' C& incubated all other plates in a rack for 48 hrs at 37'C.
Day 2 of osmotic pressure lab
we compared thickness of d growth lines for each organism across the 5 plates. Halobacterium salinarium had its optimal growth at d highest NaCl concentration at 25%
what happened as we increased radiation?
growth went down (decreased )
names of disinfectants & antiseptics used in control of growth lab are
Lysol & bleach (disinfectants ) & antiseptics includes Isopropyl Alcohol & Hydrogen peroxide
The reults of day 2 of control of growth using antiseptics & disinfectants
Observe the bottom of each plate,looking at the area of the culture around each filter paper disk for a clear zone of inhibition measure & record d diameter in mm.In our result above,notice some colonies growing within the zone of inhibition,which means they're probably resistant to d chemicals
anticeptics used were hydrogen peroxide & isopropyl alcohol & disinfectants used were lysol & bleach
yeast we used in lab
Saccharomyces cerevisiae aka bakers yeast
define coliform
All facultative anaerobe,gram negative rod that produce acid & gas from lactose fermentation in 48 hrs at 35'C
indicator organism
slime mold used in lab
physarum polycephalum
many heads
How were mold samples collected?
agar plates were left open for 5mins outdoors and for 20 mins indoors to collect spores
An MPN (Most Probable Number) greater than 0
presumes d presence of coliforms & renders water unportable
portable water must ve an MPN of 0
stain used to view mold structures was
lactophenol cotton blue stain
selective medium for molds was
Sabouraud agar plates
has low ph & high sugar hence it's selective for molds & inhibits bacteria
medium for slime mold was
tap water agar,to rehydrate sclerotium.Oats were used for feeding
molds & yeasts belong to the
Dimain Eukarya & the kingdom fungi
penicillin,aspergillus,rhizopus & coccidiodes
are natural soil fungi but can cause opportunistic diseases known as mycoses when a host is immunocompromised
plasmodium & sclerotium
plasmodium is d yellow living metabolizing form at center while sclerotium is d dormant dry form
slime mold physarum polycephalum
halophiles re categorized based on salt tolerances as
facultative halophile (staphlococcus epidermidis lives on our skin & had d widest range of tolerance )& obligate halophile(Halobacterium salinarium )
why is green sheen produced by E.coli?
becos of d acid,when it ferments,it makes d acid
3 steps of water testing
presumptive test (used dilutions & pipetted out water into tubes,observed yellow color for positive),confirmed test (used streak plate tech to culture bacteria onto Eosin Methylene Blue(EMB) agar,incubated it,then identify if it's Enterobacter aerogenes(purple colonies) or E.coli (green metallic sheets).Completed test (final stage)
A confirmed test is necessary due to acid & gas produced separately by two or mixed bacteria in a tube hence giving false positive result.
positive coliform result must have both acid & gas(significant amount of bubble) present in the tube.