Lab Quiz 4

Front 1

Bacterial growth

Back 1

an increase in the number of cells

Front 2

How to bacteria reproduce

Back 2

asexually by binary fission

Front 3

What are the two types of bacterial growth

Back 3

quantitative- number of bacterial cells present

qualitative- type of bacteria present

Front 4

generation time

Back 4

the time it takes for a population of bacteria to double

Front 5

What are the 4 phases of growth

Back 5

log phase, lag phase, stationary phase, and death phase

Front 6

Log phase

Back 6

bacterial cells are metabolically active, but havenot started reproducing

Front 7

Lag phase

Back 7

cells are reproducing at exponential rate

Front 8

Stationary phase

Back 8

rate of cell reproduction is equal to therate of cells dying

Front 9

Death phase

Back 9

cells dying off at exponential rate due to lack of nutrients

Front 10

What are some examples as to why you would need to quantify bacteria

Back 10

Dairy manufacturers need to know the number of bacteria present after pasteurization

Front 11

Spectrophotometer

Back 11

measures the turbidity of liquid samples

More light transmitted through liquid sample = lower cell countLess light transmitted through liquid sample = higher cell count

Front 12

Membrane filtration method

Back 12

A count of the visible colonies on the membrane surface gives thenumber of viable microorganisms

Front 13

Cell count plate

Back 13

1. Each colony was started by a single cell-colony forming unit (CFU)

2. Count the number of colonies to get an idea of how many cells were placed on the plate.

3. Multiply number of colonies by dilution factor to get the number of cells in the original sample.

Front 14

Aerotolerance

Back 14

providing the needed oxygen requirements for cultures

Front 15

Aerobic

Back 15

organisms require oxygen for cellular respiration, 2 types.

Front 16

Obligate aerobes

Back 16

need oxygen at atmospheric levels

Front 17

Microaerophiles

Back 17

use oxygen for respiration, but at a lowerlevel than in the atmosphere

Front 18

Anaerobic

Back 18

do not use oxygen for cellular respiration, 3 types.

Front 19

Obligate anaerobes

Back 19

do not use oxygen for respiration andcan not grow in the presence of oxygen

Front 20

Aerotolerant anaerobes

Back 20

do not use oxygen for respiration,but can grow if oxygen is present

Front 21

Facultative anaerobes

Back 21

can grow with or without oxygen

Front 22

Capnophile

Back 22

bacteria that require an increased carbondioxide environment (3-10%)

Front 23

Thioglycolate broth

Back 23

used to grow anaerobes

Front 24

Cultural Characteristics on Agar Plate are...

Back 24

shape, margin, elevation, size, texture, appearance, pigment, optical property

Front 25

Different shapes:

Back 25

circular, rhizoid, irregular, filamentous, spindle

Front 26

Different margins:

Back 26

entire, undulate, lobate, curled, rhizoid, filamentous

Front 27

Different elevations:

Back 27

flat, raised, convex, pulvinate, umbonate

Front 28

Cultural characteristics on agar slant:

Back 28

tapered, lobed, tapered and beaded, beaded, branched, rhizoid

Front 29

Coccus

Back 29

spherical bacteria, capable of dividing in any direction

Front 30

Strepto

Back 30

chain

Front 31

tetrad

Back 31

4 that look like a box

Front 32

sarcina

Back 32

organized but overlapping, coccus

Front 33

staphylo

Back 33

cluster

Front 34

bacillus

Back 34

rod shaped bacteria, divides end to end.

Front 35

palisade

Back 35

bacillus overlapping

Front 36

Spirillum

Back 36

wavy shape, rigid

Front 37

spirochete

Back 37

coiled or cork screw shape, flexibl

Front 38

vibrio

Back 38

comma shaped, slightly curved rod