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Organelles that are always present in monera

- Cell Wall


- Cell Membrane


- Ribosome


- Cytoplasm


- Nuclear material

Organelles that are sometimes present monera

- Capsule


- Flagellum


- Plasmid

Types of Shapes of Monera

Spherical, Rod and Spiral

Define and describe the process of Binary Fission

Process by which bacteria reproduce asexually


1. Chromosome replicated


2. Cell grows + the 2 chromosomes are pulled apart


3. When doubled in size, the bacterial cell splits to form 2 new cells

Types of Autotrophic Bacteria

- Photosynthetic:


- Chemosynthetic

Types of Heterotrophic Bacteria

- Saprophytic


- Symbiotic

Types of Symbiotic Bacteria Relationships

1. Mutualism


2. Commensalism


3. Parasitism

Factors that affect growth of bacteria

Oxygen, temperature, pH, external solute concentration, pressure

How does temperature affect growth of bacteria?

Bacteria have enzymes which can be denatured at certain temperatures



How does pH affect the growth of bacteria?

Bacteria have enzymes which can be denatured at unsuitable pHs



How does external solute concentration affect growth of bacteria?

- If environment outside cell has low solute concentration, water enters cell


- If environment outside cell has high solute concentration, water will leave the cell


- Bacteria cannot reproduce due to lack of water


- Basis of food preservation via osmosis

How does pressure affect growth of bacteria?

- High pressure damages cell walls

Describe the formation and workings of an endospore

- Forms in unfavourable conditions


- DNA replicates


- A tough, resistant wall forms around DNA of the cell


- Endospore remains dormant until favourable conditions + absorbs water + the wall of the spore bursts

Examples of Beneficial Bacteria

- Streptomyces


- Lactobacillus


- Escherichia Coli

Examples of Harmful Bacteria (usually pathogenic)

- Salmonella Typhi


- Vibrio Cholera


- Mycobacterium Tuberculosis

Define Pathogenic Bacteria and give example

Bacteria that causes diseases e.g. Salmonella

Define Antibiotics

Chemicals produced by microorganisms which kill or prevent the growth of other microorganisms



Where is the gene that makes microorganisms resistant to antibiotics located?

In the plasmid of the bacterial cell

What is antibiotic resistance caused by and describe how they form?

Over use of antibiotics as they kill beneficial + harmful bacteria. Bacteria in the body become used to this antibiotic + build up resistance to it

Monera are the only ________ kingdom

Prokaryotic

Describe the Growth Curve for Bacteria

1. Lag Phase: Bacteria are adjusting to new environment


2. Log Phase: Rapid increase (exponential growth) in numbers as conditions become favourable. Bacteria multiplying rapidly by Binary Fission


3. Stationary: No increase or decrease in numbers. Competition for food, oxygen and space. Toxins building up


4. Decline/Death Phase: Bacteria die - same reasons as Stationary above


5. Survival: Some bacteria live on as Endospore (only formed in unfavourable conditions)

L arry


L ost


S tinky


D irty


S ocks

Define Bioreactor

A vessel where cells, enzymes + organisms are placed to produce a specific product

Batch Flow Method (Characteristics and Example of Use)

- Microorganisms go through normal 5 phase growth curve


- Fixed amount of nutrients added at start


- Bioreactor emptied at end of production


e.g. Used to make the antibiotic Penicillin

No. of letters

Ways microorganisms are grown in bioreactors for commercial use

Batch Flow Method and Continuous Flow Method

Batch Flow Method (Definition)

A fixed amount of nutrients is added to the bioreactor and a specific amount of product is emptied at the end of production

Continuous Flow Method (Characteristics and Example of Use)

- Microorganisms kept in Log Phase to produce huge numbers


- Nutrients continuously fed into bioreactor


- Product continuously removed


e.g. Used to make a protein substitute from bacteria called Single Cell Protein (SCP)

Continuous Flow Method (Definition)

Bacteria are kept in Log Phase of growth in bioreactor as nutrients are continuously added and waste is continuously removed

Define Industrial Microbiology and give Methods

Producing bacterial products on industrial scale


Methods: Batch Culture + Continuous Culture

Benefits and Disadvantages of Batch Culture Normal Growth Curve

Benefit: Easy to control


Disadv: Needs to be sterilised + set up fresh for each 'batch'

Benefits and Disadvantages of Continuous Culture Growth Curve



Benefit:


- Kept at log or stationary phase (nutrients continuously added, wastes continuously removed)


- Can run for long periods


Disadv:


- Maintaining constant conditions (pH, nutrients) is difficult

Function of cell wall

Structure and shape


Function of cell membrane

Allow substances to enter and exit the cell


Function of ribosome

Produces protein


Function of cytoplasm

Holds organelles in place


Definition and function of nuclear material in monera

Single chromosomes of DNA that is not in a membrane and contains genetic info


Definition and function of capsule in monera

Semisolid layer around the cell wall for protection in pathogenic bacteria

Function of flagellum

Movement

Define plasmid in monera

Loop of DNA containing genes for special traits e.g. antibiotic resistance

Latin for spherical monera

(s) Coccus (pl) Cocci

How do spherical monera occur? (groups, alone, etc...)

Occur singularly in clusters, in pairs or in chains

Example of spherical monera

Pneumonia

Latin for rod monera

(s) Bacillus (pl) Bacilli

How do rod monera occur? (e.g. alone, in groups, etc...)

Occur singularly or in chains


Do spherical monera have flagella?

No

Do rod monera have flagella?

Yes

Example of rod monera

Tuberculosis

Latin for spiral monera

(s) Spiral (pl) Spirilla

How do spiral monera occur? (alone, groups, etc...)

Occur singularly

Example of spiral monera

Syphillis

Define photosynthetic and give example

Bacteria that use sunlight to make their own food


e.g. Bacteria in soil

Define chemosynthetic and give example

Bacteria that make their own foods using energy from chemical reactions


e.g. Bacteria in volcanic pools

Define saprophytic and give example

Bacteria that obtain food from dead, organic matter


e.g. Leaves

Define symbiotic

Bacteria that live off another organism where at least one of them benefits

Define mutualism and give example

Both organisms benefit from living off each other


e.g. Vitamin producing bacteria in the large intestines

Define commensalism and give example

Only one organism benefits, but doesn't cause harm to the host


e.g. Bacteria (Staphylococcus aureus) on the skin

Define parasitism and give example

Only one organism benefits + causes harm to the host


e.g. Cholera causes disease to humans

Define obligate aerobic

Organisms require oxygen for growth

Define Obligate Anaerobic

Organisms require oxygen to be absent for growth

Define Facultative Anaerobes

Can grow with or without oxygen

At what temperature do most bacteria grow best at?

Most bacteria grow best between 10 - 30°C

At what pH do most bacteria grow best at?

Most bacteria grow best at a neutral pH 6-8

What is an endospore resilient to?

Heat + chemicals

Function of Streptomyces

Produce antibiotics

Function of Lactobacillus

Produce yoghurt and cheese

Function of Escherichia Coli

Produce vitamin K

What pathogenic bacteria causes typhoid?

Salmonella typhi

What pathogenic bacteria causes cholera?

Vibrio cholera

What pathogenic bacteria causes tuburculosis?
Mycobacterium tuberculosis

What is the main benefit of bacteria?

Antibiotics


What do antibiotics not affect?

Viruses or fungi