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7 Cards in this Set
- Front
- Back
what is chromatography |
substances are separated acording to relative affinity (attraction) to stationary and mobile phase. if stronger affinity for mobile phase the solute moves quickly if stronger affinity for stationary phase the solute moves slowly |
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what type of stuff are described as stationary |
sillica (SiO2) and alumina (Al2O3) are regarded as polar due to the nature of their surface structure |
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what type of stuff regarded as mobile |
non-polar solvents;alkanes polar solvents;water,alcohols |
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describe thin layer chromatography (TLC) |
-stationary;powder -mobile;solvent -for colourless samples view results under UV light -amino acids can be stained using ninhydrin |
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describe column chromatography |
- large scale TLC -stationary ;powder -mobile;solvent |
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describe gas chromatography |
-long coiled tube packed with powder -inert gas passes through tube -retention time;time to travel through tube -samples often analysed by mass spectrometer as they leave the tube. |
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describe practical 12 |
picture |