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7 Cards in this Set

  • Front
  • Back

what is chromatography

substances are separated acording to relative affinity (attraction) to stationary and mobile phase.


if stronger affinity for mobile phase the solute moves quickly


if stronger affinity for stationary phase the solute moves slowly

what type of stuff are described as stationary

sillica (SiO2) and alumina (Al2O3) are regarded as polar due to the nature of their surface structure

what type of stuff regarded as mobile

non-polar solvents;alkanes


polar solvents;water,alcohols

describe thin layer chromatography (TLC)

-stationary;powder


-mobile;solvent


-for colourless samples view results under UV light


-amino acids can be stained using ninhydrin

describe column chromatography

- large scale TLC


-stationary ;powder


-mobile;solvent

describe gas chromatography

-long coiled tube packed with powder


-inert gas passes through tube


-retention time;time to travel through tube


-samples often analysed by mass spectrometer as they leave the tube.

describe practical 12

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