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11 Cards in this Set
- Front
- Back
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Lots of in vitro assays provide good info in a reductionist controlled experiment but |
Have lost the structure, cellular interaction and complexity of the system as well as requiring manipulation to reveal function |
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What does genetic engineering allow us to do |
Add remove specific genes Provides us with lots if models of different disease like arthritis to Tb and malaria |
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Why is it important to know stuff about certain cells |
Response to vaccination Effect of drugs Presence of infection Diagnosis of disease |
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What might we want to know about a cell and what steps are required to test these things experimentally? |
Isolate cells Evaluate number of cells Are cells proliferating? Are cells viable/dying? can cells carry out their functions? |
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Isolating cells approches can have what |
A very mixed population of cells with different cell types |
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How do you separate white blood cells from peripheral blood |
Density centrifugation |
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How to know quantification of cells |
Hemocytometer Flow cytometry |
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What causes a t cell to divide |
Antigen specific responses Mitogen induced responses |
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3 approches to measure proliferation |
Enzymes Radiation Fluorescence |
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What happens when cells die and what does this allow |
They loose their membrane integrity Allow vital dyes to enter the cell and stain them |
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Ab based technologies that measure cells functions |
Elsia elisapot flow cytometry |
