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26 Cards in this Set

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  • Back

Nutrients needed.

Nitrogen, phosphorus, silicon, iron, vitamins, light.

Phosphorus

Used for structure; phospholipids in membrane; linkers in DNA.

Sources of phosphorus

Inorganic P - as limiting as N in freshwater; when dissolved, usually 0-2 um




Organic P - degraded to phosphate by extracellular phosphatase


- Alkaline in marine spp.


- Acidic in freshwater spp.

Is nitrogen accumulated as DIN or DON?

DON, except in low concentrations within in vacuoles, most are organics (amino acids, nucleic acids, proteins)

How is P accumulated?

Polyphosphate (inorganic P) in high concentrations.

How much P is needed in relation to N?

1/10

Which is more limiting, N or P?

P

Define 'quota'

The amount of material per unit of cell or carbon

What is growth rate related to?

Quota of limiting nutrients; N, P and vitamins

How can quota behave?

- Range from Qmax to Qmin


- Exceed Qmax up to Qabs, but them not affect K (therefore is not limiting)

P is highly concentrated, involved in energetics, therefore...

Rapid recycling can compensate for low P concentration.

What do the quota curves for P and N look like?

P has a steep start and plateau, it reaches Qmax quickly, whereas N has a more shallow curve.

Is growth a function of internal or external nutrients?

Internal. If there is an absence of external nutrients, growth continues until Q = Qmin

What is KQ?

Growth rate quota

What does KQ describe?

The curve shape, irrespective of the range of Qmin, Qmax and Qabs.

What is silicon (Si) used for in diatoms?

- Frustules


- Cell division

Previously assimilated Si can't be redistributed within the cells, therefore...

Quota relationship does not apply.

What happens under non-Si limitation?

Cell cycle is prolonged, more Si is deposited, therefore Si:C increases.

What is silicate used for in diatoms?

Cell walls


- Rigid


- Overlapping valves


- These move apart during growth


- New valves made inside existing ones



How and where do diatoms have an advantage?

In high nutrient, upwelling waters; kept from sinking; Si supply.

What [compound] is [Si] similar to?

NO3-

What happens during the G1 phase?

Cell expansion

What happens during the G2 phase?

Cells prepare for division

What happens during the M phase?

Mitosis and cell division

What happens in Si limited cells?

Less Si per cell


- Spines (SSi) disappear


- Girdle (GSi) gets larger but cell cannot divide

What happens in N limited cells?

- More spines


- Retain more Si per cell