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24 Cards in this Set
- Front
- Back
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What does PS require? |
- Light - DIC - Photosystems |
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What is a photosystem? |
- A light harvesting complex (Chl a + accessory pigments) - Generates reductant, ATP - Splits H20 to release H+ |
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What is a light reaction? |
Generation of chemical energy from light |
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What is a dark reaction? |
Use of chemical energy to fix CO2 |
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What is a PE curve? |
A photosynthesis irradiance curve |
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What is the slope of a PE curve? |
The function of light reactions - the steeper, the better |
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What is the plateau of a PE curve? |
The function of dark reactions - the higher, the better |
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What is the difference between net and gross PS? |
Respiration rate |
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What is compensation point? |
Respiration rate = photosynthesis |
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Is there more pigment at high or low light? |
Low light |
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What is compensation depth? |
Where net PS = 0 |
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What is critical depth? |
Where depth-integrated PS = respiration rate. It relates to movement through tht water column where there is too much light at the top, and not enough at the bottom of the mixing zone |
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Describe a method of measure PS using 14C incubation |
- Add H14CO3 - Cells fix the 14CO2 and are therefore labelled as 14C - Filter cells; measure radioactivity in algae using scintillation counter |
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Describe a method of measure PS using O2 evolution |
Measure [O2] in water before and after incubation by colorimetric assay |
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What should be utilised as a control when measuring PS? |
Dark incubation |
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What do chloroplasts provide? |
Carbon |
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What is the importance of DIC? |
Substrate for RuBisCO is CO2 |
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What is the dominant form on DIC in water? |
HCO3- |
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What is carbonic anhydrase responsible for? |
- Generating CO2 outside of cell to bring in - Bringing in HCO3- and generating CO2 inside |
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What is basal respiration? |
The background level associated with ion balance |
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What is metabolic respiration? |
Cellular anabolic and catabolic activities that increase with synthesis and growth rate |
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What is N needed for? |
RuBisCO and enzymes |
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What are N and P needed for? |
Structure and operation of photosystems |
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What is Fe needed for? |
Photosystems and respiration |
