The Temperature At Which Enzyme Activity Is Most Effective Essay

1017 Words Oct 13th, 2015 null Page
The temperature at which enzyme activity is most effective is approximately at 40℃. This result was expected as this trial temperature was closest to internal body temperature, 38℃, and as catalase is present in all body organs (T. Fouad, Antioxidants).
Temperature affects chemical reactions by increasing the kinetic motion of molecules and changing the rate of collisions between them. In lower temperatures, kinetic motion is decreased and there are not enough collisions between the molecules for enzyme activity to be maximized. However, as temperature reaches above 40℃, the excessive motion of the enzyme begins to break the hydrogen bonds that are holding it’s 3D structure together (D. Fraser, 55) and the enzyme begins to denature. This is reflected in the results of this experiment, as can be seen in Figure 4 of the observations section. In the first three trials, activity increased as temperature increased. Activity reaches its maximum rate in this experiment at 40℃, approximately at the point where enzyme activity is most effective. In the final trial, activity decreases at 70℃. The enzyme is likely to have denatured somewhere along this point. The substrate concentration at which enzyme activity is approximately the most effective is at 5 mL H2O2 and 0 mL H20. Rate of reaction increases with substrate concentration until a saturation point is reached, where all available active sites have binded to an enzyme (D. Fraser, 52). However, in this experiment substrate…

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