The Alamar Blue Cell Viability Reagetion
Cancer can be defined as a special kind of illness that occurs when the cells in a given part of a body begin to grow out of proportion than is normal. Currently, in the United States, this disease is the second leading cause of deaths. Human beings over the history have had tumors and remedies have been implemented, and others continue to be performed and studied over time (American Cancer Society, 2010). These methods of treatment include chemo-preventive methods and of late natural products and compounds extracted from plants, and other organisms are being used as anticancer agents (Deepa, Samundeeswari, & Nirmala, 2011). The evidence of cancer has been found in ancient fossils and bones in Egypt.
There are four types of tumors that are inexistent in the world today. They include Carcinomas, which are cancer of the organs; Sarcomas, which are cancer …show more content…
An essential ingredient to be utilized in the process is the Alamar Blue Cell Viability Reagent, which is known for its reducing function. The declining ability of a cell is directly proportional to the quantitative measure of its proliferation activity. In the context, determination of the reducing capacity of a given cell will play a critical role in the establishment of its relative cytotoxic activity. The analogy is based on the idea that cells maintain a reducing environment while in their cytosol state. Because the Alamar blue reagent contains resazurin as its active ingredient, it is used in the reduction of resorufin on the subjected cells in the experiment. Resazurin is a non-toxic and non-fluorescent cell-permeable compound, which has a blue consistency. Conversely, Resofurin is a highly fluorescent red colored compound. When the components interact, viable cells are actively involved in the conversion of resazurin to resofurin. The event triggers an increase in the fluorescence and the color of the media surrounded