Essay about Recombinant Dn Dna And Dna

1949 Words Dec 5th, 2016 8 Pages
Recombinant DNA is genetically engineered DNA that is formed by splicing fragments of DNA. Organismal cloning is the artificial creation of a new organism that is genetically identical to its counterpart. DNA cloning is a recombinant DNA technique where cDNAs and fragments of genomic DNA are inserted into a cloning vector and maintained during growth of the host cells. Vector is an agent that transfers genetic material into a cell or organism. Restriction enzymes are bacterial enzymes that find restriction sites and cleaves DNA. restriction enzymes and DNA ligase are utilized to produce recombinant DNA molecules. DNA ligase catalyzes formation of 3′ and 5′ end phosphodiester bonds. Plasmids are extrachromosomal DNA molecules capable of independent replication. The gene of interest determines a gene’s structure. Polymerase chain reaction or (PCR) is the amplification of a piece of DNA into million copies of a particular DNA sequence. Gel electrophoresis is a laboratory method used to separate macromolecules. Also, used in the production of recombinant DNA and DNA cloning restriction fragments are fragments of a DNA molecule that are cleaved by a restriction enzyme. Early in the 1970s, Fred Sanger developed a sequencing technique known today as Sanger sequencing. Sanger Sequencing is the use of dideoxynucleotides to terminate newly synthesized DNA fragments at specific bases. Dideoxynucleotide triphosphates (ddNTPs) serves as the chains terminators, with the higher…

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