Microbiology : Laboratory Theory And Application Lab Manual Essay

2128 Words Oct 7th, 2014 9 Pages
Abstract
Experiment 3-14 of the Microbiology: Laboratory Theory & Application lab manual was conducted to utilize the various morphological characteristics of organisms for the identification of unknown broth culture sample #26. Staining techniques including the gram stain and capsule stain were applied to initially describe the sample as a gram-positive staphylococci bacterium. Upon further examination and comparison with the gram-positive control, the microorganism was correctly described as having ovoid-shaped streptococci cell morphology, implicating that sample #26 was Lactococcus lactis. As this microorganism is essential to the food industry and has potential uses in the healthcare industry, proper identification of L. lactis through the staining practices used in this experiment will prove useful for further research of this microbe.
Introduction
Through the application of various staining techniques, morphological characteristics can be used as clues for the identification of an unknown microorganism. This practice can be applied to a variety of settings outside of the microbiology laboratory, ranging from hospitals to food processing plants. The food industry is particularly reliant on microorganisms, both for food production and preservation. Lactic Acid Bacteria, a group of microorganisms that are part of the order Lactobacilles, produce lactic acid as the result of fermentation. They are often utilized for their metabolic properties to produce fermented dairy…

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