Genetic Mutations Of The Adenine Pathway Based On The Complementation Testing Of Different Mating Types

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This study was conducted to identify the location of genetic mutations in the adenine pathway based on the complementation testing of different mating types. Complementation testing was done through the fusion of mating type a strains wild type, B, C, D and α strains ade1, ade2, and ade8. The strains were plated onto a rich medium agar and incubated for three days at 30 degrees Celsius. Using the replica plating method the fused strains were then plated onto an adenine deficient medium and cell growth was observed. Growth of the cells at the different fusion sites was indicative of complementation; lack of growth indicated the location of the mutation. Strain B did not grow when fused together with ade2. This indicated a lack of complementation and also indicated the location of the genetic mutation. Similarly, strain C did not grow when fused with ade8 strain D did not grow when fused with ade1. These data reveal that a functional gene can complement a mutated one in order to correct a possible phenotype. This is important in the study of genetic mutations and malignant diseases.
Genetic heterogeneity occurs when a mutation is caused by different genetic mechanisms or by genes at different loci (National Genetics and Genomics Education Centre). Genes interact with one another to allow for proper functioning of biochemical reactions and for proper synthesis of proteins (Beadle and Tatum 1941). Because of the relationships between genes, complementation test…

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