Recombination and mutation are the motivation of evolution. Recombination is critical for repairing DNA lesions and for chromosomal pairing, and exchange during meiosis (Krejci et al. 2012). Recombination does not occur uniformly on the chromosomes of eukaryotes. Meiotic recombination in well studied yeast revealed that non-uniformity of recombination was observed when the frame of reference is an entire chromosome, multigene region and a pair of genes or a small region upstream of a gene (Lichten and Goldman 1995). The correlation between genetic distance and physical distance called recombination rate, it was varied within different species and genome regions, while the recombination rate enhances the gene mapping resolution and help introduce
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1994). By chance, the intragenic recombination may be occurred in the recombination hotspots or cold spots (Mourad et al. 1994). Tto identify the intragenicthis recombination, restriction fragment length polymorphism (RFLP) markers within target genes were used to distinct two parental alleles in traditional genetics . The intragenic recombination causes a distinct fragment pattern from both parents (Okagaki and Weil 1997). To easily discover the intragenic recombination, two individuals carried non-functional alleles of targeted genes, whose intragenic recombination causes a functional allele and obtained a distinct phenotype change, are priority used to develop a population for estimation of intragenic recombination rate. The functional alleles/plants in the progeny are resulted from intragenic recombination, and the ratio of functional allele plants to mutation allele plants was deemed as intragenic recombination rate.For example, the wxgene encodes a granule-bound starch synthase required for the synthesis of amylose in both the endosperm and pollen of maize and rice (Shure et al. 1983; Wang et al. 1990).Wxpollen grains stain deep blue in I2KI solution, whereas wxpollen grains stain light brown. The large sample of pollen and the easily
1994). By chance, the intragenic recombination may be occurred in the recombination hotspots or cold spots (Mourad et al. 1994). Tto identify the intragenicthis recombination, restriction fragment length polymorphism (RFLP) markers within target genes were used to distinct two parental alleles in traditional genetics . The intragenic recombination causes a distinct fragment pattern from both parents (Okagaki and Weil 1997). To easily discover the intragenic recombination, two individuals carried non-functional alleles of targeted genes, whose intragenic recombination causes a functional allele and obtained a distinct phenotype change, are priority used to develop a population for estimation of intragenic recombination rate. The functional alleles/plants in the progeny are resulted from intragenic recombination, and the ratio of functional allele plants to mutation allele plants was deemed as intragenic recombination rate.For example, the wxgene encodes a granule-bound starch synthase required for the synthesis of amylose in both the endosperm and pollen of maize and rice (Shure et al. 1983; Wang et al. 1990).Wxpollen grains stain deep blue in I2KI solution, whereas wxpollen grains stain light brown. The large sample of pollen and the easily