Genotype Lab

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Purpose: The purpose of this simulation is to demonstrate how alleles work and practice the skill of figuring out what genotype is dominate based off of the phenotype of the offsprings.

Background info: Fruit flies or Drosophila melanogaster work best in experiments because Drosophila melanogaster has 60% of genes that are mutated, amplified, or deleted in human diseases. Drosophila melanogaster also has a short lifespan which makes them best for quick experiments. They also mate and develop quickly, making it easier to study the offsprings. Drosophila melanogaster also have distinguishable characteristics that make telling the differences and similarities easier to see.

Hypothesis: When I crossed Wild type and Vestigial Drosophila and 1191/1191 were wild type, my hypothesis is that Wild type is dominant and Vestigial is recessive. Using a punnet square I predicted that there was a 75% chance of them being Wild type and
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Fist I ordered a female that had a certain phenotype and a male that had a different phenotype. The female’s genotype was DD when it was Wild type and tr. when it had a black body and sepia eyes. The male’s genotype was dd when it was Vestigial and TR when it had a tan body and red eyes. After mating the flies and waiting “Two weeks” which was really a few seconds for the offsprings to develop, I sorted the flies under the microscope. I then counted the offsprings and how many were Wild type and how many were Vestigial and later with the offsprings of the Drosophila melanogasters that had tan and black bodies with sepia and red eyes. F1’s genotype, which is the offspring of DD and dd had 74% wild type and 26% vestigial. F2’s genotype, which is the offspring of tr (black and sepia) and TR (tan and red) was varying from rt .08%, Rt 19%, rT 18%, and RT 55%. We then mated F1 with each other and did the same with

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