Dna Extraction Essay

614 Words Sep 28th, 2005 3 Pages
DNA EXTRACTION

In extracting chromatin from the cells of wheat germ there are seven steps to

follow. The optimal cell to use would be the polyploidal eukaryotic. Eukaryotes

have nucleus membrane-bound organelles, while prokaryotic does not. The

polyploidal eukaryotic cell has DNA that is held in the nucleus while the

prokaryote has DNA that floats freely around the cell. The DNA of eukaryotes is

more complex and extensive than the other. Prokaryote is a bacterial cell that

usually has DNA in one large strand and only has one chromosome while the

eukaryotic cell has more than one chromosome and is considered to be a higher

organism. Prokaryotes have an outer wall that prevents them from bursting or
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When a protein loses it normal configuration it is called denatured. The

tenderizer must eliminate the proteins, the membrane and envelope allowing the

DNA to free itself from the tight coil. In the last step, you add ice-cold alcohol.

When you add the freezing alcohol to the hot test tube you will cause

precipitation . Water is a polar molecule and when mixed with alcohol

the ethanol's reaction with the DNA changes it from a polar to a nonpolar state.

Therefore, it is stuck between two polar liquids and it forms its own layer.

The DNA is not soluble in the alcohol unlike the soap. Only the DNA that directly

contacts the alcohol becomes nonpolar and is near the top can precipitate out of

the detergent but stay under the alcohol, where it is not soluble and therefore you

can see the strand of DNA. There are three steps that were completed:

homogenization, deprotenization, and precipitation of the DNA.

Basically, the extraction could not occur without all the steps being followed and

the chemical reactions that each part causes. The hot water, cold water, baking

soda, alcohol, and meat tenderizer all play a part in the chemical process that

DNA extraction requires. Without one you couldn't obtain the DNA. The research

Was interesting and I learned more about the cell functions by doing this paper.

1. Mader, Sylvia . (2001). Biology (7th edition). McGraw-Hill, Inc.
2. www.

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