Dna Extraction Essay
In extracting chromatin from the cells of wheat germ there are seven steps to
follow. The optimal cell to use would be the polyploidal eukaryotic. Eukaryotes
have nucleus membrane-bound organelles, while prokaryotic does not. The
polyploidal eukaryotic cell has DNA that is held in the nucleus while the
prokaryote has DNA that floats freely around the cell. The DNA of eukaryotes is
more complex and extensive than the other. Prokaryote is a bacterial cell that
usually has DNA in one large strand and only has one chromosome while the
eukaryotic cell has more than one chromosome and is considered to be a higher
organism. Prokaryotes have an outer wall that prevents them from bursting or …show more content…
tenderizer must eliminate the proteins, the membrane and envelope allowing the
DNA to free itself from the tight coil. In the last step, you add ice-cold alcohol.
When you add the freezing alcohol to the hot test tube you will cause
precipitation . Water is a polar molecule and when mixed with alcohol
the ethanol's reaction with the DNA changes it from a polar to a nonpolar state.
Therefore, it is stuck between two polar liquids and it forms its own layer.
The DNA is not soluble in the alcohol unlike the soap. Only the DNA that directly
contacts the alcohol becomes nonpolar and is near the top can precipitate out of
the detergent but stay under the alcohol, where it is not soluble and therefore you
can see the strand of DNA. There are three steps that were completed:
homogenization, deprotenization, and precipitation of the DNA.
Basically, the extraction could not occur without all the steps being followed and
the chemical reactions that each part causes. The hot water, cold water, baking
soda, alcohol, and meat tenderizer all play a part in the chemical process that
DNA extraction requires. Without one you couldn't obtain the DNA. The research
Was interesting and I learned more about the cell functions by doing this paper.
1. Mader, Sylvia . (2001). Biology (7th edition). McGraw-Hill, Inc.