Analyzing The Nucleic Acid Is The Polymerase Acid Of The Bacteria ( Aacc )

834 Words Nov 30th, 2015 4 Pages
Diagnosis

The most appropriate test to detect Neisseria gonorrhoeae is the nucleic acid amplification test, also know as NAAT. NAAT consist of a urine sample, endocervical and vaginal swab for women, and a urethral swab in men (CDC, 2015). Test detects the genetic material of the bacteria, in this case N. gonorrhoeae. Theoretically the test is able to detect a single copy of the nucleic acid of the bacteria (AACC, 2014). The test amplifies the number of copies of the genetic material in order for the bacteria to be identified. One of the ways microbiologist amplify nucleic acid is the polymerase chain reaction (PCR); It is based on the DNA polymerase to synthesize a new strand of DNA that is complementary to the template strand provided. There is also the ligase chain reaction (LCR). The nucleic acid is used as the probe. Two probes are used per each DNA strand and are ligated together to form one single probe. This method uses a DNA polymerase and a DNA ligase (Lefts, 2004). Although NAAT is able to detect the presence of N. gonorrhoeae, it is not able to detect if it is resistant to antibiotics (AACC, 2014). Furthermore, because of this issue a culture is also needed to detect resistance to antibiotics. For a culture test a endocervical or urethral swab specimen is needed. Specimen can also be taken from the rectum and pharyngeal. The specimen is streaked onto a Thayer-Martin or Martin Lewis selective medium. It can also be streaked onto a chocolate agar nonselective…

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