Diagnosis
The most appropriate test to detect Neisseria gonorrhoeae is the nucleic acid amplification test, also know as NAAT. NAAT consist of a urine sample, endocervical and vaginal swab for women, and a urethral swab in men (CDC, 2015). Test detects the genetic material of the bacteria, in this case N. gonorrhoeae. Theoretically the test is able to detect a single copy of the nucleic acid of the bacteria (AACC, 2014). The test amplifies the number of copies of the genetic material in order for the bacteria to be identified. One of the ways microbiologist amplify nucleic acid is the polymerase chain reaction (PCR); It is based on the DNA polymerase to synthesize a new strand of DNA that is complementary to the template strand provided. There is also the ligase chain reaction (LCR). The nucleic acid is used as the probe. Two probes are used per each DNA strand and are ligated together to form one …show more content…
Furthermore, because of this issue a culture is also needed to detect resistance to antibiotics. For a culture test a endocervical or urethral swab specimen is needed. Specimen can also be taken from the rectum and pharyngeal. The specimen is streaked onto a Thayer-Martin or Martin Lewis selective medium. It can also be streaked onto a chocolate agar nonselective medium and incubated at thirty-five Celsius to thirty-six Celsius and with a five percent of carbon dioxide. A gram stain and oxidase test is then preformed and positive results will show a gram negative, oxidase positive diplococcic bacteria. (Johnson, 2002). Chlamydia trachomatis is a disease that has similarities to N. gonorrhoeae. Both infections are spread through a strain of bacteria that is spread through sexual contact. Both infection can be spread through the baby during birth. Also, both chlamydia and gonorrhea might not show any outward visible symptoms. The procedure for testing for either infections is the same and in most cases will be treated with the same type of medication (Chico,
The most appropriate test to detect Neisseria gonorrhoeae is the nucleic acid amplification test, also know as NAAT. NAAT consist of a urine sample, endocervical and vaginal swab for women, and a urethral swab in men (CDC, 2015). Test detects the genetic material of the bacteria, in this case N. gonorrhoeae. Theoretically the test is able to detect a single copy of the nucleic acid of the bacteria (AACC, 2014). The test amplifies the number of copies of the genetic material in order for the bacteria to be identified. One of the ways microbiologist amplify nucleic acid is the polymerase chain reaction (PCR); It is based on the DNA polymerase to synthesize a new strand of DNA that is complementary to the template strand provided. There is also the ligase chain reaction (LCR). The nucleic acid is used as the probe. Two probes are used per each DNA strand and are ligated together to form one …show more content…
Furthermore, because of this issue a culture is also needed to detect resistance to antibiotics. For a culture test a endocervical or urethral swab specimen is needed. Specimen can also be taken from the rectum and pharyngeal. The specimen is streaked onto a Thayer-Martin or Martin Lewis selective medium. It can also be streaked onto a chocolate agar nonselective medium and incubated at thirty-five Celsius to thirty-six Celsius and with a five percent of carbon dioxide. A gram stain and oxidase test is then preformed and positive results will show a gram negative, oxidase positive diplococcic bacteria. (Johnson, 2002). Chlamydia trachomatis is a disease that has similarities to N. gonorrhoeae. Both infections are spread through a strain of bacteria that is spread through sexual contact. Both infection can be spread through the baby during birth. Also, both chlamydia and gonorrhea might not show any outward visible symptoms. The procedure for testing for either infections is the same and in most cases will be treated with the same type of medication (Chico,