Analysis Of Adenosine Receptor On The Electrophysiology Of Hctp / Or Producing Neurons Of The Hypothalamus

1198 Words Mar 22nd, 2015 null Page
Analysis of Adenosine receptor blockage’s effects on the electrophysiology of HCTP/OR producing neurons of the Hypothalamus – receptor blockage mediated by Caffeine

Introduction
In this experiment, the quantification of firing rate changes on a specific neural sub-group will be analyzed via whole cell patch-clamping and the application of the common psychoactive drug-caffeine. The significance of quantifying the electrical signaling of the chosen neuron is to proportionally quantify the effects the chosen drug-caffeine has on the neurotransmitter Adenosine. Caffeine is a known antagonist of Adenosine, inquiring that it counters Adenosine’s inhibitory effects by a simple mechanism of competitive inhibition – not allowing Adenosine to bind to its corresponding receptor. The chosen sub-group of neurons to be patched are Hypocretin/Orexin Neurons, for they possess an Adenosine receptor, more specifically A1; as well as their location, being in the Lateral Hypothalamus – a much more simple location for cell isolation and patch-clamping (von Kitzing, 1994).
By quantifying the change in firing rate, the quantification of channels can be proportionally analyzed. This is due to Adenosines role in inhibiting the CNS whenever present – thus applying varying levels of caffeine one should see an increase in firing rate of the neurons that are directly affected by Adenosine. This again is due in part to the role of neurotransmitters themselves. They act as a signaling system between…

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