204 nasopharyngeal samples were collected 11.3 percent were swabs, while the other 88.7 were aspirates/wash. A result of findings did not differentiate between collection methods usage. In 187 samples, at least one pathogen was found during molecular diagnostics (PCR). However 73 of the PCR positive samples that came up positive were initially negative in immunofluorescence assays. The most commonly detected virus in the PCR method in the largest to smallest order was: RSV, hRV, hBoV, AdV, HMPV, IV, and hPIV and hCoV with the same values. A pattern with co-infected samples showed the most frequent combination of viruses to be: RSV with hRV, then RSV with hBoV, and last RSV with Adv. RSV was observed with the same frequency as a single infection and as a co-infection, whereas hRV, IV, hBoV, AdV, and hMPV were more frequent in co-infection …show more content…
In the GENDRES cohort, RSV and hRV were both the most common viral infection detected, but the UK cohort’s most common viral infections were RSV+hBoV and IV+hBoV. The differences in most common viral infection results in comparison to region of study was resulted because the UK patients were recruited in 2009 during the pandemic influenza season, along with epidemiology. Bocavirus, a newly discovered virus that may cause ARI’s with the highest frequency found in infants in the hospital. The results in the experiment showed that hBov is commonly detected, third in GENDRES study and fourth in UK study, in respiratory samples of young children with LT-ARI. Several studies show that children without ARI’s can still show viral identification in the patient, however further research is necessary to understand the respiratory viral carriage and infection. PCR quantification is possible but respiratory samples are heterogeneous and different extractions could result in diverse results depending on variation in the amount of virus present in the aliquots extracted and examined. Detection rates ranged from 47% to 95% expressing the more than likely margins of error throughout the experiment: i. genetic variability and predisposition in the studied populations, ii. differences in upper or lower respiratory symptoms at