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81 Cards in this Set

  • Front
  • Back

Abnormal variation in size of erythrocyte.

Anisocytosis

Abnormal variation in shape of the erythrocyte?

Poikilocytosis

Erythrocyte with a diameter of 9-12 microns.

Macrocytes

RBC shows a peripheral time of hemoglobin with a dark staining central area.

Target cell

RBC with the bull’s eye appearance.

Thalassemia


Liver disease


Post splenectomy

⬆️ in iron in the mitochondria of normoblasts.

Sideroblastic anemia

Chronic blood loss?

Iron deficiency

Iron deficiency anemia

Low serum iron, high TIBC

Polycythemia vera

Pancytosis

Not typically seen in the peripheral smear in megaloblastic anemia.

Spherocytes

⬆️ in osmotic fragility in RBCs.

Hereditary spherocytosis

Contain iron stained granules.

Siderocytes

Remnant of nuclear DNA in RBC?

Howell - Jolly body

RNA remains in RBC when stained with?

New methylene blue

Cabot rings found in?

Erythrocytes

Excess membrane for cell volume (bull’s eye).

Target cell

Decreased cell membrane with same cell volume.

Spherocytes

Pitting” of red blood cell inclusion by spleen.

Bite cell

Crystallization of abnormal hemoglobin during oxygen deprivation.

Sickle cell

RNA inclusion.

Reticulocyte

DNA inclusion?

Howell - jolly body

Iron inclusion?

Pappenheimer body

Hemoglobin inclusion?

Heinz body

Decreased white cells, red cell and platelets.

Pancytopenia

Granulocytes released prematurely to myelocyte stage.

Left shift

⬆️ polychromasia?

Reticulocytosis

WBC count >50,000 /ul?

Leukomoid reaction

WBC inclusion?

Dohle bodies

G6PD deficiency.

Hexose monophosohate shunt (HMP)

Pelger huet anomaly.

2 lobes & hyperclumped chromatin

Lupus cells (LE), most probable source of confusion?

Tart cells

Maybe confused with a shift to the left?

Pelger-Huet

Stain that differentiates AML from ALL?

Peroxidase

Philadelphia chromosome.


A low neutrophil alkaline phosphatase NAP/LAP is seen in?

CGL (Chronic granulocytic leukemia)

Auer rods.

Acute granulocytic leukemia

Increased titer or antinuclear antibody (ANA) aids in the diagnosis of?

SLE

Reed steinberg cells

Hodgkin’s disease

Increased basophils and or eosinophils in the early stages.

Chronic myelocytic leukemia

Leucopenia in >50% of ptxs with >90% massive splenomegaly is most likely?

Hairy cell leukemia

Peroxidase is negative for?

Lymphocytes

Most chronic lymphocytic leukemias are neoplasm of?

B lymphocytes

Giant, bizarre platelets, nRBCs & teardrop red blood cells?

Myelofibrosis

Last red blood cell precursor to retain the nucleus before it becomes an erythrocyte?

Metarubricyte

Stain positive for hairy cell leukemia?

ACP

Hodgkin’s disease.

High WBC count with neutrophilia, high ESR, low serum iron

*Requires fresh samples?

Myeloperoxidase & LAP

*Present in neutrophils but NOT in monocytes?

NAP/LAP

*RBC histogram

MCV & RDW

*ALL is diff from AML

ALL is negative to both peroxidase & esterase

*Appropriate screening for HbS

Sodium dithionite test

*Maturation time: 3-5 days, including tissue phase: 121 days

Erythrocytes

*In 1% NaCl solution, what will happen to the RBC.

Crenate

*Computed in automation

MCHC

*Computed in automation

MCHC

*Measures anisocytosis

RDW

*Measures variation in platelet size

PDW (measure of uniformity of platelet size)


MPV (average size of platelets)

*Megakaryocytic stage with platelet shedding

Late metamegakaryocyte (mature metamegakaryocyte: 2000-4000 plts)

*Common test for used in patients with hereditary form of hemolytic anemia

OFT (increased)

*Done to further determine anemia

Blood film studies + MCHC

*Test for Bone marrow failure

RBC & Retic

*Sausage shaped nucleus

Band cell

*D shaped nucleus

Myelocyte

*Formation of DMS through cytoplasmic invagination

Promegakaryocyte

*Directly measured by electrical impedance

RBC, WBC, Platelet

*Stem cell to blast: 5 Life span in tissue: 10 days

Monocyte

*Bone marrow smear is prepared by?

Crush


Concentrate


Particulate

*How to make a good smear

Smooth

*What can be made in automated smear maker?

Wedge

*What can be made in automated smear maker?

Wedge

*Failure to create secondary enzymes


*Hyposegmentation

Pelger Huet

*Differentiate CML from Leukomoid

LAP score of more than 100

*Increase in WBC

Strenous exercises


Emotion


Crying

•Stains ALP present in the neutrophil


•Increased in infections with neutrophilia (leukomoid reaction)


•Decreased in CML


•Fresh capillary blood specimen

LAP (Leukocyte Alkaline Phosphatase)

•Used to differentiate AML & Monocytic leukemia from ALL


•Fresh blood smear is the specimen

Myeloperoxidase Stain

•Differentiate AML & myelomonocytic leukemia from ALL

Sudan Black B

•Stains mucoproteins, glycoproteins.


•Used in the diagnosis of FAB M6 (DGS)


•L1 & L2 produce a block pattern

Periodic Acid Schiff Stain

•Stains esterases in granulocytes & mast cell granules


•Differentiate granulocytic cells from monocytic cells

Naphthol AS -D Choroacetate Esterase

•Marker for Hairy Cell Leukemia

TRAP (Tartrate resistant ACP)

•Useful for the recognition of mast cells & tissue basophils

Toluidine blue

•Screening procedure for the detection of chronic granulomatous disease


•Heparanized whole blood is the specimen

Nitroblue Tetrazolium Neutrophil Reduction Test

•Present in 90% cases of ALL

Terminal Deoxyribonucleotidyl Transferase (TdT)