Microbiology Lab 3 Quiz

Front 1

Catalase (-) enzyme

Back 1

Does not have enzyme

No bubbling

EX: Entercoccus faecalis

Front 2

Catalase + micrococci

Back 2

Should bubble

Ex: Staphylococcus epidermis

Front 3

Catalyst (-)

Back 3

Microbes _________ enzyme do NOT produce gas bubbles

Front 4

Catalyst enzyme +

Back 4

Microbes with ________ produce oxygen gas bubbles when H2O2 is broken down

Front 5

Fermentation

Back 5

Metabolic pathway which microbes produce ATP by breaking down glucose in absence of O2

Front 6

Acid ( pyruvic acid)

Gas ( carbon dioxide)

Alcohol

Back 6

Fermentation typically results in 3 things

Front 7

pH indicator

Back 7

Production of acid can be detected by _________

Front 8

Phenol red ( PR) broth contains

Back 8

Peptone ( protein)

Lactose ( carb)

Duham tube

pH indicator ( phenol red)

Front 9

Deamination

Back 9

Process of bacterial break down of peptone

Produces NH3 ( ammonia base)

*removal of an amino acid

Front 10

Lactose carbohydrates

Back 10

Bacteria are lactose fermenter

Splits disaccharide lactose into glucose & galactose

Front 11

Lactose

Back 11

Ferments glucose & galactose produces ATP , pyruvic acid & CO2 gas

Front 12

Durham tube

Back 12

Tiny upside down tube collects CO2 gas bubbles produces during fermentation

Front 13

Yellow & acid

Back 13

If pH is less than 7 : what color & type

Front 14

Orange red

Neutral

Back 14

If pH is equal to 7

What is Color & type

Front 15

Base & pink

Back 15

If pH is greater than 7

Front 16

Catalase test

Back 16

Test identifies organism that produces catalase enzyme

Front 17

Catalase

Back 17

Enzyme necessary to convert H2O2 to water & O2

Front 18

H2O2

Back 18

Highly potent cellular toxin that forms during respiration pathways that invole oxygen

Front 19

Aerobes

Microaerophile

Facultative anarobes

Back 19

Microbes that use O2 pathways are classified as:

Front 20

Protective enzymes

Back 20

Catalase & superoxide dismutase are:

Front 21

Catalase & superoxide dismutase

Back 21

Allow organism to live in presence of O2

Front 22

Differential test media

Back 22

Provide specific growth conditions where by organism carries out a reaction allowing it be distinguished from bacteria

Front 23

Differential test media

Back 23

Used to differentiate Enterobacter species from each other and from other Gram (-) rods based on ability to ferment carbohydrates

Front 24

Phenol Red Broth

Back 24

_____________ is a differential fermentation media

Front 25

E Coli in Phenol red test

Back 25

gram -

Pink

Front 26

Control in Phenol red test

Back 26

Gram -

Red

Front 27

S Flexnari in Phenol red test

Back 27

Gram +

Yellow

Acid

No gas

Front 28

K Pneumoniae
in Phenol red test

Back 28

Gram +

yellow

no gas

Front 29

S typhumurium

Back 29

Gram +

pink

produces NH3

Front 30

Bacteria in Catalase test

Back 30

Staphlococcus Epidermidis catalase +

Enterococcus faecalis catalase -

Front 31

Which bacteria had Good growth and which had bad growth in HE agar test?

Back 31

All were good growth ( E coli, S flexnari, K pneumonia, S typhmrium) EXCEPT for E faecalis

Front 32

Color of growth for E Coli in HE Agar test

Back 32

Pi ( pink to orange)

Front 33

Color of growth for E faecais in HE Agar test

Back 33

None, no growth present

Front 34

S flexnari color of gowth in HE Agar test

Back 34

Bppt ( Blue-green with black precipitate)

Front 35

color of growth for k pneumonia in HE Agar test

Back 35

Pi( pink to orange)

Front 36

S
Typhumurium

Back 36

B ( blue green without black precipiate)

Front 37

if you were trying to differentiate between Salmonella and Shigella which ingredient would need to be included in your media

Back 37

Substrate ( ferric ammonium citrate and sodium thiosulfate)

Front 38

Is the control plate in HE Agar test lab, positive or negative and why?

Back 38

Positive control, set to show + results bacteria growth without inhibitor

Front 39

When examining the results from all bacteria in HE Agar test, which bacteria is most helpful?

Back 39

E. Faecalis- because it has to show good culture

Front 40

HE agar is both what type of media

Back 40

Selective and differential

Front 41

selective media contain 4 components

Back 41

Nutrition

Inhibitor

Substrate

Indicator

Front 42

selected to obtain optimum growth pf suspected organisms

Back 42

Nutrition

Front 43

prevent or inhibit growth of some microbes

Back 43

Inhibitor

Front 44

agent added that allows some bacteria to preform specific chemical reactions

Back 44

substrate

Front 45

make desired reaction visable

Back 45

Indicator

Front 46

HE agar contains what?

Back 46

Lactose ( nutrion)

Bile ( inhibitor)

Ferric ammonium citrate & sodium thiosulfate ( substrate)

Bromthymol Blue dye ( indicator)

Front 47

Lactose ( nutrition )

Back 47

Encourages growth of K pneumoniae and E coli

Front 48

Bile ( Inhibitor)

Back 48

inhibits growth of Gram +

Front 49

Ferric ammonium citrate & sodium thiosulfate ( substrate)

Back 49

form black precipiate in bacteria tht reduces sulfur

Front 50

Salmonella

Back 50

sulfur reduction ( +)

Front 51

Shigella

Back 51

sulfur reduction (-)

Front 52

Bromthymol blue dye (Indicator )

Back 52

measures pH changes turns yellow in acid

Front 53

E Coli is gram - or +

Back 53

Gram -

lactose fermenter

Front 54

E faeclais

Back 54

Gram +

Front 55

S Flexnari
In agar test

Back 55

Gram -

sulfur reducer

non lactose fermenter

Front 56

K pnemoniae

Back 56

Gram -

lactose fermenter