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13 Cards in this Set
- Front
- Back
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In Starch hydrolysis, what is the hydrolytic enzyme used to break down starches?
What does it break down into? |
Amylase
Maltose and glucose |
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In Casein hydrolysis, what is the hydrolytic enzyme used to break down casein (in milk)?
What does it break down into? |
Caseinase
Amino acids |
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In Gelatin hydrolysis, what is the hydrolytic enzyme used to break down gelatin (in food and animal skin)?
What does it break down into? |
Gelatinase
Amino acids |
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In Lipid hydrolysis, what is the hydrolytic enzyme used to break down lipids (glycerol + 3 fatty acids)?
What does it break down into? |
Lipases
Smaller components, glycerol and fatty acids |
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What is a positive result for a gelatin hydrolysis test?
Negative? |
Liquid
Solid |
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What is a positive result for a casein hydrolysis test?
Negative? |
Positive: Clear halo around bacterial growth
Negative: NO halo around bacterial growth |
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What is a positive result for a starch hydrolysis test?
Negative? |
Positive: Clear halo around bacterial growth
Negative: dark color with NO halo effect |
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What is a positive result for a Tween 80 lipid hydrolysis test?
Negative? |
Positive: Pink solution
Negative: No color change from yellow/orange |
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What refers to the pathogens that can cause disease present in food?
What refers to microorganisms present on food that cause it to be inedible? |
Food poisoning
Food spoilage |
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What is a method which will reveal the number of bacteria per unit volume of the original sample?
How does it work? |
Serial dilution
By diluting a fixed amount of the original stock sample several times, a tenfold dilution |
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What is the spread plate technique?
A single bacterial colony on the plate is assumed to originate from one viable cell of the stock solution. This is called a... |
Plating a small amount from the dilution blank
Colony forming unit (CFU) |
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What is the equation for a dilution factor (df)?
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df= volume of sample added/total volume (sample + blank)
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How many colonies are needed for a plate to be statistically relevant?
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30-300 colonies
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