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13 Cards in this Set
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- Back
- 3rd side (hint)
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Streak plating |
Technique used to isolate a pure strain from a single species of microorganism. |
Culture can be grown on a new plate so that the organism can be identified, studied, or tested. |
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Incubate upside down |
Plates are incubated upside down (agar up), so that condensation does not drop onto the plate and interfere w/ the developing microbes. |
So condensation does not drip |
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EMB |
Eosin methylene blue- selective differential medium used to isolate fecal coliforms. |
Eosin methylene blue |
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PEA |
Phenyl lethyl alcohol- isolation of gram positive staphylococcus species & streptococcus species from clinical specimens that contain mixture of bacterial flora. |
Gram positive staphylococcus species & streptococcus species that contain mixture of bacterial flora. |
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Blood agar |
Used for isolation & cultivation of many types of fastidious bacteria also used to differentiate bacteria based on their hemolytic characteristics. |
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MSA |
Mannitol salt agar- both selective and differential medium used in isolation of staphylococci. Contains 7.5% NaCl, thus selects for bacteria that can tolerate high salt concentrations. |
7.5% NaCl, thus selects bacteria that can tolerate high salt concentrations. |
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MAC |
Macconkey agar- select for gram negative, kills gram positive, differential agar for lactose fermenters. |
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MIO |
Motility, indole, ornithine decarboxylase |
Motility, indole, ornithine decarboxylase |
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Kovac's reagent |
Kovac's reagent is a biochemical reagent consisting of Isoamyl alcohol. It is used for the diagnostical indole test, to determine the ability of the organism to split indole from the amino acid tryptophan. |
Biochemical regent consisting of Isoamyl alcohol. |
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UREA |
Urease broth is a differential medium that tests the ability of an organism to produce an exoenzyme called urease, that hydrolyzes urea to amonia & carbon dioxide. |
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Kirby Bauer |
Antibiotic testing. Uses antibiotic -impregnated wafers to test whether bacteria are affected by antibiotics. |
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Phenol red test |
Uses a pH indicator that turns from red to yellow as the pH goes from alkaline to acidic. |
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Methylene blue |
Simple stain used for animal cells to make nuclei more observable. |
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