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28 Cards in this Set
- Front
- Back
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what lead to the development of the microscope?
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better lenses
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hooke coined what word? while studying what?
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cell, dead cork cells
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Schleiden (1838) concluded what?
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plants are made of cells
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Schwann (1839) concluded what?
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animals are made of cells
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what is Cell Theory
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all living things are formed by
the division of existing cells |
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Light microscopy Resolution is
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0.2 um
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light microscope samples can be
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dead or alive
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light microscope samples must be
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relatively transparent
and thin |
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if light microscope samples are to thick what do you do?
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section
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in light microscopes it must have contrast, how do you get contrast?
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staining and use of different optics
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Fluorescence
microscopy Resolution is what? |
0.2 um
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in fluorescent microscopy cellular structures are
labeled with? |
fluorescent
molecules like dyes, and antibodies |
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fluorescent molecule is
1. |
excited
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fluorescent molecule is 2.
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emits light of
a different wavelength |
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Light illuminates entire field of view
• Out-of-focus light compromises the image which microscope has these characteristics? |
Fluorescent microscopy
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which microscopy has these characteristics?
• Clear image is created by focusing laser beam on individual points. • Ideal for live and thicker samples |
Confocal microscopy
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Confocal (fluorescent) microscopy
Resolution is? |
0.2 um
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Transmission electron microscopy (TEM) resolution is?
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2 nm
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in TEM what is used to create and focus the image.
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Electrons and magnets
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to create contrast in TEM
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Dead samples are sectioned
and stained with electron dense materials |
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Scanning electron
microscopy (SEM) Resolution is? |
3-20nm
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in SEM Heavy metal‐coated, dead samples are scanned by
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an electron beam
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in SEM Pattern of electron scatter generates a ?
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a 3D‐like image
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characteristics of prokaryotic cells?
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Lack nuclei and other
membrane bound organelles • Single‐celled organisms |
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what are the two type of prokaryotic cells?
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bacteria and archea
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example of prokaryotic cells?
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E.Coli
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characteristics of eukaryotic cells?
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Contain nuclei and
other organelles Found in single or multi‐ cellular organisms |
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example of eukaryotic cells?
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humans
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