Bios 351: Aseptic Technique

Front 1

Why is it necessary to incubate plates upside-down?

Back 1

prevents condensation from falling on to agar and spreading inoculated organisms

Front 2

When inoculating a broth tube, what should be done after inserting the loop (containing organisms) into the sterile broth? In other words, what is the proper way to complete the inoculation?

Back 2

twist loop several times to disperse the organisms

Front 3

Why is the aspetic technique important?

Back 3

-insures no contamination organisms are introduces into culture material
-insure organisms don't contaminate the handlers
-insures no contamination remains after you have worked with cultures

Front 4

What does disinfecting the work area do?

Back 4

destroys vegetative cells and viruses but may not destroy endospores