1)Hands were washed thoroughly with soap and water to ensure that no contamination on the coli-MUG tubes. The sealed plastic package was opened and coli-MUG tubes were removed.
2)Usually, inner fermentation vials contain only air. The assemblies was inverted and inner vials were allowed to fill with air, only if these inner vials contain any liquid.
3)Cap was removed and it was ensured that open end of the tube or the inside of the cap did not open.
4)Each of the given tubes were dispensed with 10 mL sample using the Pipette. The tubes were filled fully with sample when approximately 10 ml was put inside.
5)The screw cap was replaced and the tube assembly was inverted a few times to mix the water sample with coli-MUG broth. The inner fermentation was ensured that it was full of liquid and no air bubbles remained.
6) The tubes were placed in the dri-bath incubator at a temperature of 35±0.5°C, or 95±1°F. After one hour, the inner fermentation vials were examined for trapped air. The assemblies were inverted briefly to allow the bubbles to escape from the inverted fermentation vials if any air bubbles were presented again. All tubes were returned to the incubator. The tubes were kept in …show more content…
Fluorescence may be detected before gas production and usually is seen within 24 hours. The tubes were covered with a box or placed in a darkened area under diffused light to check for fluorescence. (too much visible light masks the presence of fluorescence). The tubes were hold under a long-wave ultraviolet (UV) light, or the UV light was hold so that it shined on the tubes. Please be informed that the germicidal lamps (short-wave UV lights) was not suitable for this purpose. The presence of Escherichia Coli. was indicated by the presence of fluorescence in a tube. If the fluorescence was not detected after 24 hours, the incubation was continued and was examined again after a total of 48±3
2)Usually, inner fermentation vials contain only air. The assemblies was inverted and inner vials were allowed to fill with air, only if these inner vials contain any liquid.
3)Cap was removed and it was ensured that open end of the tube or the inside of the cap did not open.
4)Each of the given tubes were dispensed with 10 mL sample using the Pipette. The tubes were filled fully with sample when approximately 10 ml was put inside.
5)The screw cap was replaced and the tube assembly was inverted a few times to mix the water sample with coli-MUG broth. The inner fermentation was ensured that it was full of liquid and no air bubbles remained.
6) The tubes were placed in the dri-bath incubator at a temperature of 35±0.5°C, or 95±1°F. After one hour, the inner fermentation vials were examined for trapped air. The assemblies were inverted briefly to allow the bubbles to escape from the inverted fermentation vials if any air bubbles were presented again. All tubes were returned to the incubator. The tubes were kept in …show more content…
Fluorescence may be detected before gas production and usually is seen within 24 hours. The tubes were covered with a box or placed in a darkened area under diffused light to check for fluorescence. (too much visible light masks the presence of fluorescence). The tubes were hold under a long-wave ultraviolet (UV) light, or the UV light was hold so that it shined on the tubes. Please be informed that the germicidal lamps (short-wave UV lights) was not suitable for this purpose. The presence of Escherichia Coli. was indicated by the presence of fluorescence in a tube. If the fluorescence was not detected after 24 hours, the incubation was continued and was examined again after a total of 48±3