Purpose:
To identify an unknown microorganism by performing a series of biochemical tests on a pure bacterial culture.
Materials and Methods:
Carbohydrate Utilization: Materials:
- Phenol red lactose Broth
- Phenol Red Sucrose Broth
- Unknown organism # 40
- Inoculating tool
Procedure:
Phenol red lactose and Phenol Sucrose were cultures used to identify fermentation of carbohydrates for the unknown organism #40. Phenol red is a ph. indicator as the inverted Durham tube indicates gas production. The unknown organism was inoculated into each phenol red broth with either sucrose or lactose. The inoculating tool was sterilized by heat between each inoculation. The inoculated broth was then incubated at 37℃ for 24-48 hours. The negative outcomes are: medium …show more content…
Unknown organism #40 was inoculated into 1% Tryptone. Inoculating tool was sterilized with heat before and after inoculation. The inoculated broth was incubated at 37℃ for 24-48 hours. Kovac’s reagent was then added into the inoculated broth after incubation period. Negative outcome of experiment is a remained yellow ring in the reagent layer on top of the broth. A positive outcome is the appearance of a red ring in the reagent layer on top of the broth.
Urea Hydrolysis: Materials:
- 1 Urea broth
- Unknown Organism #40
- Inoculating tool
Procedure:
Urea broth contains phenol red a ph. indicator, urea and yeast extract to detect the hydrolysis of urea. Unknown organism #40 was inoculated into the Urea broth. Inoculating tool was sterilized with heat before and after inoculation. The inoculated broth was incubated at 37℃ for 24-48 hours. Positive outcome of experiment is the color change from orange to magenta. The negative outcomes are no color change or a color change of orange to
To identify an unknown microorganism by performing a series of biochemical tests on a pure bacterial culture.
Materials and Methods:
Carbohydrate Utilization: Materials:
- Phenol red lactose Broth
- Phenol Red Sucrose Broth
- Unknown organism # 40
- Inoculating tool
Procedure:
Phenol red lactose and Phenol Sucrose were cultures used to identify fermentation of carbohydrates for the unknown organism #40. Phenol red is a ph. indicator as the inverted Durham tube indicates gas production. The unknown organism was inoculated into each phenol red broth with either sucrose or lactose. The inoculating tool was sterilized by heat between each inoculation. The inoculated broth was then incubated at 37℃ for 24-48 hours. The negative outcomes are: medium …show more content…
Unknown organism #40 was inoculated into 1% Tryptone. Inoculating tool was sterilized with heat before and after inoculation. The inoculated broth was incubated at 37℃ for 24-48 hours. Kovac’s reagent was then added into the inoculated broth after incubation period. Negative outcome of experiment is a remained yellow ring in the reagent layer on top of the broth. A positive outcome is the appearance of a red ring in the reagent layer on top of the broth.
Urea Hydrolysis: Materials:
- 1 Urea broth
- Unknown Organism #40
- Inoculating tool
Procedure:
Urea broth contains phenol red a ph. indicator, urea and yeast extract to detect the hydrolysis of urea. Unknown organism #40 was inoculated into the Urea broth. Inoculating tool was sterilized with heat before and after inoculation. The inoculated broth was incubated at 37℃ for 24-48 hours. Positive outcome of experiment is the color change from orange to magenta. The negative outcomes are no color change or a color change of orange to