Objective
I. To learn how to inoculate the nutrient broth properly in preventing contamination to the nutrient broth and the culture Escherichia Coli. This is done by sterilized the loop and sterilized the neck of the tub with heat from before use and before put the cape on.
II. To learn how to do a simple streak of an E. coli culture on a nutrient agar plate with one stroke from start to end like a “Z”.
III. To learn how to do a complex streak where we grow E. coli culture on the nutrient agar plate for differential the isolate colonies presence.
IV. To learn how to know whether the culture is a selective or differential media. The culture bacteria that will be using are E. coli, Bacillus subtilis, staphylococcus aureus and Proteus vulgaris.
V. To learn how to find the extracellular enzyme production by bacteria. The culture bacteria that will be using are E. coli, Bacillus subtilis, and staphylococcus aureus.
Result
i. The two …show more content…
What is the purpose of heating the neck of a tube when aseptically transferring a sample?
_The purpose of this is to obtain an isolate culture and prevent the culture from being containment with other organism around us such as from our hand or on the tube. This is done by heat the neck of the tube every time when it’s open and when it will close while the loop is being sterilize so we can control the bacteria that is being transfer or mixt with the culture.
2. Why should you never label the lid of an agar plate?
_Because the bottom of the agar plate contain our experiment and if the lid get lost we can tell what organism that is growing on the plate than label the lid and when it get lost we will not be able to identify which organism that is grow on the plate.
3. When doing the complex streak, how do you ensure you do not incinerate the bacterial cells with the inoculating loop?
_The inoculating loop should be cool down first after sterilizing so it will not kill the culture that is need for the
I. To learn how to inoculate the nutrient broth properly in preventing contamination to the nutrient broth and the culture Escherichia Coli. This is done by sterilized the loop and sterilized the neck of the tub with heat from before use and before put the cape on.
II. To learn how to do a simple streak of an E. coli culture on a nutrient agar plate with one stroke from start to end like a “Z”.
III. To learn how to do a complex streak where we grow E. coli culture on the nutrient agar plate for differential the isolate colonies presence.
IV. To learn how to know whether the culture is a selective or differential media. The culture bacteria that will be using are E. coli, Bacillus subtilis, staphylococcus aureus and Proteus vulgaris.
V. To learn how to find the extracellular enzyme production by bacteria. The culture bacteria that will be using are E. coli, Bacillus subtilis, and staphylococcus aureus.
Result
i. The two …show more content…
What is the purpose of heating the neck of a tube when aseptically transferring a sample?
_The purpose of this is to obtain an isolate culture and prevent the culture from being containment with other organism around us such as from our hand or on the tube. This is done by heat the neck of the tube every time when it’s open and when it will close while the loop is being sterilize so we can control the bacteria that is being transfer or mixt with the culture.
2. Why should you never label the lid of an agar plate?
_Because the bottom of the agar plate contain our experiment and if the lid get lost we can tell what organism that is growing on the plate than label the lid and when it get lost we will not be able to identify which organism that is grow on the plate.
3. When doing the complex streak, how do you ensure you do not incinerate the bacterial cells with the inoculating loop?
_The inoculating loop should be cool down first after sterilizing so it will not kill the culture that is need for the