Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
23 Cards in this Set
- Front
- Back
|
what is the mutation we are working with
|
vir2
|
|
|
what does the mutation affect?
|
the second virescent mutant that was isolated in arabidopsis thaliana and is a nuclear recessive mutation that affects chloroplast development
|
|
|
what is the mutant phenotype
|
leaves become green much more slowly that the leaves of wildtype plants and the leaves are never as fully green as wildtype levels of chlorophyll.
|
|
|
what does RFLP stand for
|
restriction fragment length polymorphism
|
|
|
how many RFLPs do we use
|
7
|
|
|
what is an RFLP
|
single base pair changes that occur in the chromosomes of the species under study
|
|
|
what are the two different ecotypes of arabidopsis
|
Landsberg (L) and columbia (C)
|
|
|
what is an ecotype
|
geographically separated populations of arabidopsis that still have the ability to be crossed together and produce normal progeny. due to nucleotide changes over time, different ecotypes have slightly different genomic DNA sequences
|
|
|
what are microsatellite markers
|
repetitive DNA based on dinucleotide repeats
|
|
|
what were the phenotypes of the parental generation that was crossed
|
vir2 columbia mutant x wildtype landsberg
|
|
|
what are the genotypes of the parental generation
|
vir2vir2 CC x vir2+vir2+ LL
|
|
|
what is the phenotype of the F1 generation
|
a combination of L and C with one member of each pair of homologous chromosomes deried from the L parent and the other from the C parent
|
|
|
what is the genotype of the F1 generation
|
vir2+vir2 CL
|
|
|
how many pairs of chromosomes does arabidopsis have. how many are sex chromosomes
|
5. 0.
|
|
|
how many of the F2 generation have the vir2 phenotype
|
one-quarter
|
|
|
why are the chromosomes of the F2 generation a mixture of C and L
|
crossing over occurs during meisois when the two C and L homologues form a tetrad. the result is that the chromosome I that enters the gametes is part L and part C
|
|
|
if an F2 plant has the vir2 phenotype what is its genotype? why?
|
vir2vir2. bc vir2 is recessive. since vir2 originally came from the C parent, than a plant that is vir2vir2 must have C DNA in the region of the vir2 gene on both homologues
it must be CC in the region of the chromosome wher the vir2 gene is located |
|
|
what is the gel visualized with
|
ethidium bromide
|
|
|
what are the ingredients to a PCR reaction
|
template DNA, deoxyribonucleotides (dNTPs), buffer, foward and reverse primers, taq DNA polymerase, and water
|
|
|
What was the total volume of each reaction
|
20 ul
|
|
|
What are the steps to a PCR reaction and what are the temperatures?
|
1. denature dna strands at 94
2. 92 3. anneal primers at 54 4. extend with taq polymerase @ 72 5. hold at 20 repeat 30 cycles |
|
|
explain the difference between RFLPs and microsatellite markers
|
cutting RLFPs with certain restriction enxymes will give us one pattern of cleavage for columbia and a different pattern for landsberg
microsatellites will have different sizes between landsberg and columbia |
|
|
what RF value determines a linkage
|
an RF<0.5 means that they are linked and that the two alleles are on the same chromosome
otherwise they are far apart or on separate chromosomes |
