Types Of Agar

Front 1

Cysteine-tellurite

Back 1

Differential for C. Diptheriae

Produces black colonies

Front 2

Loeffler

Back 2

Enrichment for C. Diptheriae

Promotes development of metachromatic granules.

Front 3

Tindale

Back 3

Selective differential for C. Diptheriae

Corynebacterium produce gray to black due to reduction of tellurite.

C. Diptheriae are surrounded by brown halo.

Front 4

Bismuth sulfite

Back 4

Salmonella

S. Thyphi are black, surrounded by metallic sheen. Others light green.

Front 5

Cefsulodin-irgasan-novoviocin (CIN)

Back 5

Selective for Yersinia enterolitica, Aeromonas, Plesiomonas shigelloides.

Crystal violet inhibits GN. Novobiocin inhibits GPC. Cefsulodin inhibits most GP and GN. Yersinia appears as red "bull-eye"

Front 6

Alkaline peptone water (APW)

Back 6

Enrichment for recovery of Vibrio from stool. Alkaline pH suppresses commensals. Subculutured to TCBS.

Front 7

Thiosulfate citrate bile salts sucrose agar (TCBS)

Back 7

Selective for vibrio. High pH inhibits most bacteria. V. Cholera ferments sucrose produces yellow colonies. V. Parahaemolyticus and V. Vulnificus don't ferment and produce blue green.

Front 8

Bordet-Gengou

Back 8

Selective enrichment for Bordatella pertussis.

Potato-glycerol-based medium enriched with blood. Contsminsnts inhibited by methicillin. "Cough plate." Bordatella resemble mercury droplets.

Front 9

Buffered Charcoal-yeast extract agar (BCYE)

Back 9

Enrichment for Legionella.

Yeast extract and L-cysteine enhance growth. Charcoal absorbs toxic compounds.

Front 10

Regan-Lowe

Back 10

Selective for B. Pertussis

Charcoal supplemented with horse blood, cephalexin, and amphotericin B.

Front 11

Human blood bilayer Tween (HBT)

Back 11

Selective and differential for G. Vaginalis.

Incubate in increase CO2 for 48 hr. Colonies are beta hemolytic.

Front 12

Modified Thayer-Martin

Back 12

Selective for N. Gonorrhea and N. Meningitidis

Vancomycin, colisitin, nystatin, and trimethoprim inhibit growth of other bacteria. Incubate in increase CO2.

Front 13

Martin-lewis

Back 13

Selective for N. Gonorrhea and N. Meningitidis

Similar to Thayer-Martin but different antibiotics. Inhibits yeast better. Incubate in increase CO2.

Front 14

New York City medium (NYC)

Back 14

Selective for N. Gonorrhea and N. Meningitidis

Incubate in increase CO2. Some N. Gonorrhea will be inhibited by antibiotics. Genital mycoplasmas will grow.

Front 15

GC-LECT

Back 15

Selective for N. Gonorrhea and N. Meningitidis

Antibiotics to inhibit GN and GP bacteria and yeast.

Front 16

JEMBEC

Back 16

For transportation and growth of Thayer N. Gonorrhea. Plates contain Neisserria selective medium and come with resealable polyethylene bag and CO2 generating tablet. No need to transfer to culture plate.

Front 17

Deoxychocolate-citrate

Back 17

Selective for Salmonella and Shigella.

Other enterics inhibited.

Front 18

Hektoen enteric (HE)

Back 18

Selective and differential for Salmonella and shigella

Bile salts, bromothymol blue, acid fuchsin inhibit normal GI flora. Nonpathogens=orange to pink. Non-lactose fermenters=green to blue-green. H2S=black center

Front 19

Xylose lysine deoxychocolate (XLD)

Back 19

Selective and differential for Salmonella and shigella.

Deoxychocolate inhibits many GNR and GP.

Yellow=E. Coli

Yellow with black center=some Proteus

Colorless/red=shigella

Red with black centers=salmonella

Front 20

Salmonella-Shigella (SS)

Back 20

Selective for salmonella and shigella.

Brilliant green and bile salts inhibit enterics. Colorless colonies. Salmonella had black center.

Front 21

Gram-negative broth

Back 21

Enrichment and selective for salmonella and shigella from stool and rectal swabs.

Deoxychocolate and citrate salts inhibit growth of GP. Subculture onto selective media after 6-8 hour & 18-24 hour incubation.

Front 22

SSA

Back 22

Selective for Group A strep. Group B will also grow.

Antibiotics suppress normal throat flora.

Front 23

Eosin methylene blue (EMB)

Back 23

Selective and differential for enteric GNR.

Eosin and methylene blue inhibit GP.

Lactose fermenters=green/black/purple

Non lactose=colorless

E. Coli=green metallic sheen

Front 24

Cycloserine-cefoxitin-fructose agar (CCFA)

Back 24

Used for recovery of C. Difficile. Inhibit growth of gram negative coliform.

C. Diff ferments fructose, forming acid that causes colonies to become yellow.

Front 25

Cetrimide

Back 25

Isolation of pseudomonas aeruginosa

Also called pseudocel

Contains pigment pyocyanin