Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
38 Cards in this Set
- Front
- Back
|
A single smooth arc of ppt forms between the antigens and antibodies,indicating ab ppt identical antigen |
Identity |
|
|
Two separate lines of ppt cross each other,indicating ag and ab are unrelated and do not ppt together |
Identity |
|
|
Two ppt lines meet forming spur.this indicate that the ag share some common epitopes |
Partial identity |
|
|
What are those examples performed by double diffusion |
Anti rana Anti rnp Anti scl Anti ss-a Anti ss-b Jo-1 antibody Ku ab Mi-1 ab Pm-1 ab |
|
|
Combine the speed of electrophoresis with sensitivity of imminodiffusion |
Electroimmunodiffusion |
|
|
Antibody is place in well favoring its migration in the direction of cathode,ag that tend to be more neg |
Electroimmunodiffuosion |
|
|
What are those example of immunologic assay performed by electro immunodiffusion |
C1q,C2,IgG subclasses |
|
|
Essentially double diffusion technique in which voltage is applied to move the ag and ab |
Countercurrent immuneelectrophoresis |
|
|
Examples of immunologic assay performed by CEI |
Antinuclear ribinuclearprotein Anti sm Radioimmunoppt Detection of pneumococcal and meningococcal ab |
|
|
Othername for Laurell technique |
Rocket electrophoresis |
|
|
Applies on electrical charge to RID assay |
Rocket electrophoresis |
|
|
Migration of charged particle in some medium(solid/liquid) |
Electrophoresis |
|
|
Positive charge particle |
Move toward cathode |
|
|
Negative charged particle |
Move toward anode |
|
|
Charged molecule |
Directly proportional |
|
|
Size of molecule |
Inversely proportional |
|
|
Increase current increase migration |
Electrical field |
|
|
Increase ionic strength decrease migration rate |
Ionic strength of buffer |
|
|
Acid pH decrease migration rate |
PH of buffer |
|
|
Increase temperature decrease migration rate |
Temperature |
|
|
Combination of electrophoresis and double immune diffusion |
Immuno electrophoresis |
|
|
Limitation |
Semiquantitative test |
|
|
Specimen of choice |
Fresh human serum or urine |
|
|
If not performed specimen |
2-8C upto 5 days of collection |
|
|
Stain |
Coomasie brilliant blue |
|
|
Albumin marker |
Bromphenol blue in aqueous solution |
|
|
Preservative |
Sodium azide |
|
|
Anti sera |
Supplied in liquid form,prepared horse ,sheep,goat,donkey,rabbit |
|
|
Applications for immune electrophoresis |
Determine qualitatively the increase decrease class IG Detect structure and conc changes in protein Decrease conc bence jones |
|
|
Two stage procedures using agarose gel protein |
Imminofixation electrophoresis |
|
|
Enumerate 2 stage using agarose gel protein |
Electrophoresis in first stage Immunoppt in second |
|
|
Specimen for I'mmunofixation electrophoresis |
Serum,CSF,urine,other body fluids |
|
|
Stain for imminofixation electrophoresis |
Protein sensitive stain-amido black |
|
|
Application for I'mmunofixation |
Diagnosis of monoclonal gammopathies |
|
|
Advantage for ria |
Highly sensitive |
|
|
Label used for ria |
Radio active isotopes |
|
|
Principle for RIA |
Competitive binding |
|
|
Disadv of ria |
Radioactive hazard Disposable problem High cost. Short half life |
