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293 Cards in this Set

  • Front
  • Back

What two helminths coexist together?

Whipworm and Ascaris lumbricoides

What in the #1 helminth parasite infection in the world?

A. lumbricoides

What is the #2 helminth parasite infection in the USA?

A. lumbricoides

How many eggs can the female A. lumbricoides lay in a day?

200,000

Where do adult A. lumbricoides live?

Small intestines; free and unattached for upto a year

How large are the adults in A. lumbricoides?

Male: 15-35cm


Female: 22-35cm

High fever and anesthesia can promote what in A. lumbricoides?

Migration



Can tangle and block intestines and migrate to other organs (liver, lungs)



Can exit through mouth, nose, and tear ducts

What are the symptoms in a light infection in A. lumbricoides?

no symtoms



to mild pain and vomiting

What are the symptoms in a heavy infection in A. lumbricoides?

Intestinal obstruction, abdominal pain, nutritional depletion



May hamper growth in young children

Where do larvae migrate in A. lumbricoides?

Through the lungs; causes an immune response: pneumonitis, cough, asthma, edema, low grade fever, eosinophilia, hives after sensitization

How do you treat a A. lumbricoides infection?

Mebendazole


piperazine

What is the laboratory diagnosis in A. lumbricoides?

Eggs in feces

What is the size/appearance of A. lumbricoides egg?

75um x 50um mammilated embryo inside, thick shell, brown bile stain



Unfertilized: 90 um x 45um thin shell



decorticated: more elongated and refractile

What is the infective stage of A. lumbricoides?

embryonated eggs

What is the geographical location that A. lumbricoides is found?

Warm climates



Areas of poor sanitation



Tropics/Subtropics



Appalachia and parts of the USA

What is the geographical location of Ancylostoma duodenale?

Southern Europe


North coast of Africa


SE Asia


S American tropics and subtropics

What is the geographical location of Necator americanus?

Souther USA


Caribbean


Central America


Nother S. America


SE Asia


Central and South America

Which hookworm has "teeth"?

Ancylostoma duodenale

Which hookworm has " cutting plates"?

Necator Americanus

What do hookworms secrete to gain nutrients?

anticoagulants; inhibiting host absorption effecting physical and mental development of children

What is the size of adult hookworms?

Male: 5-11mm


Female: 9-13mm

What is the diagnostic stage in hookworm?

Eggs in feces

What is the infective stage in hookworm?

Filariform larvae

How is the host infected with hookworm?

By penetrating skin

How is the host infected in A. lumbricoides?

Ingestion of soil-contaminated hands or food

The initial infection with hookworm is associated with what symptom?

"Ground itch"


or


"Dew itch"



initial small red itchy papule on feet

Where do hookworm larvae migrate?

Into lungs and no host sensitization



Can result in intra-alveolar hemorrhage and mild pneumonia, cough, sore throat, bloody sputum, and headache in heavy infection

Where are adult hookworms found in the host?

small intestines

What are the clinical symptoms in an acute hookworm infection?

iron-deficiency anemia (microcytic, hypochromic)


pica


enteritis, pain, weakness, dizziness

What are the clinical symptoms in a chronic hookworm infection?

slight anemia


weight loss


non-specific symptoms


Usual form of infection

What do hookworm eggs look like?

55-75um x 35-40um clear, thin shelled, multicelled embryo within

What is the treatment for hookworm?

Mebendazole


Pyrantel pamoate

What does the adult A. duodenale look like?

teeth in buccal cavity


10-12mm

What does the adult in N. americanus look like?

cutting plates in buccal cavity


7-10mm

What is the geographical location in Strongyloides stercoralis?

tropical


subtropical

What is another name for Strongyloides stercoralis?

theradworm

What are the two forms of S. stercoralis?

Parasitic- small female (2-3mm) in intestinal mucosa; can cause unisexual reproduction



Free-living- assures perpetuation of parasite when no host is available

What is the infective form of S. stercoralis?

Filariform larvae: 630um x 16um

What is the diagnostic form of S. stercoralis?

Rhabditiform larvae: 380um x 20um

What are the three forms of S. stercoralis?

Direct


Indirect


Autoinfection

What is a direct infection of S. sterocarlis?

penetrates the skin just like hookworm

What is an indirect infection of S. sterocralis?

free living adult in soil

What is an autoinfection of S. sterocralis?

the rhabditiform larvae are delayed in passage and mature to infective form

What is a hyperinfection of S. sterocralis?

1. entry


2. spread via blood


3. infection- to lungs


4. egg deposited in mucosa


5. disease: pneumonia, malabsorption, mucosal damage, diarrhea


6. larvae exit

What clinical manifestation is common in a S. stercralis infection?

eosinophilia

What is the treatment for a S. stercoralis infection?

Ivermectin

What does the rhabditifrom larvae of S. stercoralis look like?

short buccal cavity "hour glass" esophagus and prominent genital primoridum

What specimens can S. stercoralis be found in?

Stool, sputum, duodenal aspirates

How does the host become infected with Trichuris trichiura?

Fecal to oral route

What is the geographical distribution of Trichuris trichiura?

In warm climates and areas of poor sanitation



Common in children and institutionalized mentally retarded

What is Trichuris trichiura commonly known as?

Whipworm

What is the adult morphology of T. trichiura

anterior portion burrows in the mucosa of the lumen of the colon



Male: 30-45mm


Female: 35-50mm

What are the clinical manifestations of a light T. trichiura infection?

asymptomatic; no treatment needed

What are the clinical manifestations of a heavy T. trichiura infection?

Bloody diarrhea with mucus


Abdominal pain and tenderness


Weight loss and weakness


Nausea, vomiting, constipation, flatulence

What is a serious complication of a T. trichiura infection?

Rectal prolapse

What is the treatment in T. trichiura?

Albendazole


Mebendazole

What is diagnostic stage of T. trichiura?

Unembryonated egg

What does the unembryonated egg look like?

"barrel-shaped" brown hyaline "plugs"

What is the most common helminth infection in the USA?

Enterobius vermicularis

What is the geographic distribution in Enterobius vermicularis?

Temperate climate


Can be found worldwide



Group infection in children common ages 5-10


Increased incident in Caucasians

What is Enterobius vermicularis also known as?

"pinworm"


"seatworm"

What is the morphology of Enterobius vermicularis?

Live in cecum of the colon



Male: 2-5mm


Female: 8-13mm

What is the treatment for Enterobius vermicularis?

Albendazole


Mebendazole

What are all the clinical manifestations of E. vermicularis associated with?

migration of the adult gravid female out from the anus to lay her egg on the perianal region at night



hypersensitivity; perianal itching



1/3 are asymptomatic, irritation of mucosa, insomnia, irritability, vaginal/vulva irritation

How is E. vermicularis diagnosed?

clear flattened "football" shaped egg containing a "tadpole-like" larvae

How are eggs recovered in E. vermicularis?

with cellophane tape

What is Taenia saginata also known as?

Beef tapeworm

How many people worldwide are infected with T. saginata?

60 million infected worldwide

What is the geographic location of T. saginata?

Common in developing countries


SW USA

Taenia solium is also called?

Pig tapeworm

What is the geographic location of T. solium?

Developing countries

How many people are infected with T. solium?

4 million

What can T. solium cause?

Cysticerosis

What geographical locations are cysticerosis found?

Mexico, Central and South America

How are humans infected with Taenia?

By eating infected or inadequately cooked pork or beef containing cysticerci

What is the infective stage of Taenia?

Cycticerci

What is the diagnostic stage of Taenia?

gravid proglottid


eggs

What are the intermediate host of Taenia spp

T. solium: pig


T. saginata: cattle

Taeniasis

infection by the adult Taenia (T. saginata or T. solium)



Causing diarrhea, constipation, indigestion

What is the infective stage of cysticercosis?

embryonated eggs of T. solium


infecting muscle and subcutaneous tissues


associated with neurological disease

What is the lab diagnosis of Taenia spp.?

31-43um diameter, brown shell



oncosphere have 6 hooks

How to diagnose cysticercosis

serology


MRI


Papiliedema (edema of the optic disk)

Adult morphology of Taenia spp.

T. solium: 3-5 meters long


T. saginata: 4-8 meters long

Where does T. saginata develop

muscle


viscera

Where does T. solium develop?

brain, skin, muscle

Taenia spp. rostellum?

T. saginata- no rostellum


T. solium- rostellum and hooks

Scolex of adult Taenia spp.

T. saginata- no hooks


T. solium- hooks

Proglottids Uterine branch in Taenia

T. saginata: 23


T. solium: 8

Passing of proglottids in Taenia

T. saginata: single, spontaneous


T. solium: in groups, passively

Ovary and Vagina in Taenia spp.

T. saginata: two lobes and present


T. solium: three lobes and absent

Treating Adult taenia spp. infection

Praziquantel


NoclosamideT

Treatment of Cysticercosis

Albendazole


Praziquantel


Must treat seziures


May require surgery

What is the geographic location of D. latum?

Countries bordering baltic sea


Russia


Switzerland


Great Lakes

What is the largest tapeworm found in man?

Diphyllobothrium latum

Host for D. latum?

Human, dog, cat, and pig

What is another name for D. latum?

Broad tapeworm or Fish tapeworm


proglottids wider than long

What are the intermediate hosts of D. latum

Copepods (coracidium larva)


Fish (pike, perch, trout)- (plerocercoid)

What is the diagnostic stage in D. latum?

Unembryonated eggs passed in the feces

What is the infective stage in D. latum?

plerocercoid larvae

What are the symptoms associated with D. latum?

Pernicous anemia- Vitamin B12 deficiency


Mostly generally mild: eosinophilia, obstruction, abdominal pain, diarrhea

Sparganosis

accidental ingestion of copepod

What is the lab diagnosis of D. latum?

Egg in stool (formylether)


58-75um x 40-50um



Has a operculum and small knob at other end



Proglottids can be found in stool

How do you treat D. latum?

Praziquantel


Niclosamide

How is the host infected with D. latum?

Eating raw or pickled fish

Hymenolepis diminuta is also called?

Rat tapeworm


Adults 20-60cm in length

Where is H. diminuta found?

In rodents/ rarely in humans

Where is H. diminuta found?

Worldwide

What does H. nana infect

Humans

Where is H. nana found?

Cosmopolitan; more common in children

What is H. nana also called?

Dwarf tapeworm

What is the most common tapeworm worldwide?

H. nana


Adult: 15-40mm in length

Intermediate host that is possible in H. nana?

insect but not needed (cysticercoid)

Where are adult H. nana found?

Small intestines

Clinical symptoms in H. nana?

asymptomatic, maybe associated with autoinfection


Treatment of H. nana?

Niclosamide

H. nana lab diagnosis?

Eggs in stool (formylether concentration)


spherical or ovoid



30-47 um in diameter


4-8 polar filaments

Echinococcus granulosus is also known as

Dog tapeworm or hydatid tapeworm

What is E. granulosus associated with?

sheep


can be associated with domestic herding dogs

What is the geographic location of E. granulosus?

SW USA, Alaska Canada


Africa, Middle East, Australia, S. America

Where is E. granulosus found?

canines, foxes, coytoes, or rodents

How is E. granulosus found in humans?

As a cyst (epithelium lined cavity containing liquid or semisolid material)


Look for protoscolexes in cyst fluid


31-43um diameter, brown shell


6 hooks in oncosphere

What are the symptoms of E. granulosus?

Cyst in liver: no symptoms until large, jaundice


Cyst in lungs: no symptoms until large enough



Anaphylactic shock with rupture


Eosinophilia, urticaria, and bronchospasm

Diagnosis of E. granulosus?

Serology


MRI



Cyst: protoscolexes in cyst fluid, cyst wall


31-43um diameter; brown shell, oncosphere has 6 hooks

Dipylidium caninum geographic distribution?

Europe, China, Philippines, Japan, Argentina


US

How does an infection occur in Dipylidium caninum?

Accidental ingestion of dog or cat flea

What is another name for Dipylidium caninum?

Flea tapeworm

What is the intermediate host in D. caninum?

Dog or cat flea larvae

What is the definitive host in D. caninum?

Dogs, cats, foxes, and children


infected when adult flea ingested

Lab diagnosis of D. caninum?

Egg packets


or gravid proglottids

What is the treatment D. caninum?

Praziquantel


Prevention: prevent flea infestation, wash hands after playing with pets

Fasciolopsis buski geographic distribution?

Asia, China, Idonesia, Taiwan, Thailand, Bangladesh, India

What is the infective stage of Fasciolopsis buski?

metacercariae

What are the intermediate hosts in F. buski?

Snail


Water plants (metaccercariae)-night soil used as fertilizer

Where is the adult F. buski located?

Small intestines

What is the diagnostic stage in F. buski?

Unembroynated eggs

What is the infective stage in F. buski?

metacercariae on water plants

Who is the host in F. buski?

Humans, Pigs

Where are the F. buski adults located?

Small intestines

What is a F. buski infection associated with?

Eosinophilia


Light infection: asymptomatic


Heavy infections: mucosal ulcers, abdominal pain, nausea, anemia, diarrhea and malabsorption, death can occur

F. buski/F hepatica lab diagnosis?

Eggs in stool:


130-150um with thin shell


indistinct operculum


filled with yolk cells

Fasciola hepatica is also called?

Sheep liver fluke

Where is F. hepatica found?

Worldwide in sheep raising areas

Who is the definitive host in F. hepatica?

Sheep


Humans accidental from eating unwashed watercress

Intermediate host in F. hepatica?

Egg embryonated in water--> miracidia


Snail (sporocyst->rediae->cerceriae)


watercress (metacercariae)

Where are adult F. hepatica found?

bile duct (migrate through intestinal wall, peritoneal, cavity, and liver into biliary ducts

What is the infective stage of F. hepatica?

metacercariae

What is the diagnostic stage of F. hepatica?

Unembroynated eggs

False fascioliasis

eating liver with Fasciola eggs

Symptoms in F. hepatica

Major Symptoms:


Mechanical irritation


toxic worm metabolites


mechanical obstruction



Fever, jaundice, eosinophilia, portal cirrhosis, bile duct obstruction, diarrhea, anemia

Treatment of F. hepatica

Triclabendazole with bithionol


bithionol

Adult fluke F. hepatica

2-3 cm long

Adult fluke F. buski

7 cm

Where are F. buski adults found?

Intestines

What does F. buski infect?

Pigs, Dogs, rabbits, humans

What is the geographic distribution of Metagonimus yokogawai

1.2% Korean


China


SE Asia


Israel


Russia


Spain


Egypt

Where are Heterophyes Heterophyes found?

Near East Far east


Parts of Africa

How are humans infected with M. yokogawai and H. heterophyes

by ingesting metacercariae in undercooked fish

What is the infective stage of M. yokogawai/ H. heterophyes?

metacercariae

Where are the adults found in M. yokogawai/H. heterophyes

Small intestines

What are the intermediate hosts in M. yokogawai/H. heterophyes?

Embryonated in water


Snail (cercariae): sporocyst--> rediae-->cercariae


Fish (encyst to outside of fish as a metacercariae)

What is the diagnostic stage in M. yokogawai/H. heterophyes?

embryonated egg

Metagonimus and Heterophyes clinical manifestation?

Worms that travel to heart or brain and produced granulomas


diarrhea


abdominal



Generally asymptomatic

How to diagnose Metagonimus/ Heterophyes

Finding eggs in stools (formylether conc)


has operculum


small knob on opposite end


Metagonimus: 26-30um by 15-20um


Heterophyes: 28-30um by 15-17um

Treatment for M. yokogawai/Heterophyes

Praziquantel

What is Clonorchis sinensis also called

oriental liver fluke

Where is C. sinensis located?

Far east: Southern China, Japan, Korea, Taiwan, Vietnam

Who is the host in C. sinensis?

Fish eating mammals

What's the most important reservoir for C. sinensis?

Dogs and cats

How are humans infected with C. sinensis?

metacercariae in undercooked fish

Where do the adult C. sinensis live?

small and medium sized bile ducts



metacercaria excyts in the duodenum


ascend biliary tract

Intermediate hosts for C. sinensis?

embryonated-->miracidia


Snail (miracidia-->sporocysts-->rediae-->cercariae)


Fish (metacercariae)

What is the diagnostic stage in C. sinensis?

embryonated eggs in feces

Clinical symptoms of C. sinensis?

up to 50 eggs asymptomatic



Heavy burden (>500 eggs) causes serious illness:


fever, diarrhea, epigastric pain, enlarged tender liver, jaundice, may invade liver---> cholecystitis

Lab diagnosis of C. sinensis?

Use an Entero-test


operculum and small apopercular knob


27-35um by 11-20um long


shell can be covered in debris

Treatment of C. sinensis

Albendazole


Praziquantel


How to prevent a C. sinensis infectiion?

Not eating raw, pickled or undercooked fish

Paragonimus Westermani is also called

Lung fluke

What is the geographic distribution of P. westermani?

Far East, China, Africa, South America

What is P. westermani associated with?

Crabs and crayfish

Where is P. westermani found?

humans and animals

How are humans infected with P. westermani?

metacercariae in undercooked crabs or crayfish

What are the intermediate hosts in P. westermani?

Unembryonated--> embryonated


Snail (miracidia-->sporocysts-->rediae-->cercariae)


Crustacean (cercariae)

What is the diagnostic stage in P. westermani?

unembryonated eggs

Where are adult P. westermani located?

Lungs; fever and inflammatory reaction; sputum blood tinged: Charcot-Leyden crystals (rusty iron fillings)



Cerebral calcifications may develop



possibly: abdominal paragonimiasis

Where can diagnostic stage be found

In stool or sputum

Clinical manifestations of P. westermani

Lung cyst: rusty sputum (Charcot-Leyden crystals)


Hemoptysis- resembles TB


Cerebral calcifications possible

Lab diagnosis of P. westermani

In sputum or stool (formylether concentrate)


80-100 um by 45-65 um


operculum and small knob



Chest x-ray or ELISA

Treatment of P. westermani

Praziquantel


bithionol

Prevention of P. westermani

Careful of eating raw, pickled, or undercooked crab or crayfish

What is the geographical distribution of Schistosoma spp

S. japonicum: China, Japan, Philippines, Indonesia



S. mansoni: W. & C. Africa, Egypt, Malagasy, Arabian penninsula, Brazil, Surinam, Venezuela,


West Indies



S. heamatobium: Africa, India, Middle East

What's the second most prevalent parasitic disease in the world

Schistosoma spp


200 million infected


800,000 deaths

Schistosoma adults

Female resides in the gynecophoral canal of the shorter more robust male

What is the infective stage of schistosoma spp

cercaria penetrates skin

What is the intermediate host in schistosoma?

(miricida hatch and enter snail)


snail (sporocysts become cecaria)


cecaria leave the snail

Where does the adult S. japonicum live?

superior mesenteric vein



a zoonosis (typical host dogs, cats, and cattle)

Schistomsoma mansoni adult lives:

Inferior mesenteric vein in the lower colon



man, baboon, and rats host

Clinical manifestations of Schistosomas

Swimmer's itch


Eggs: granulomas and ulceration


Eggs in portal vein cause: portal hypertension and splenomegaly



Katayama fever- High fever, hepatosplenomegaly, lymphadenopathy, eosophilia, dysentary

Is mansoni or japonicum more severe?

Japonicum

Diagnosis of Schistosoma

Eggs in stool or rectal biopsy (formylether conc)



S. mansoni: lateral spine 114-175um



S. japonicum: 68-100um long small lateral spin

Another name for swimmer's itch

Cecarial dermatitis

What do adult S. haematobium live?

inferior mesenteric veins

Where are S. haematobium eggs deposited?

in the wall of the bladder

What is the definitive host for S. haematobium?

Humans

S. haematobium clinical manifestations

  • Swimmers itch
  • Adults in veins near bladder: hematuria and proteinuria
  • Eggs: Granulomas, Bladder thickened (abnormal bladder fxn, urinary infection, kidney damage)

What is chronic S. haematobium infection associated with?

Squamous cell bladder cancer

Laboratory findings for S. haematobium

From bladder biopsy, urine sample, rectal snip:


iodine stain


terminal spine

Treatment of Schistosoma spp

Praziquantel


avoid exposure to water harboring snails

Entamoeba histolytica/dispar geographic distribution?

amebic dysentary


worldwide


contaminated water


outbreaks in households or institiuutions

Clinical manifestations of Entamoeba histolytica/dispar

asymptomatic



10% develop invasive disease; dysentary, extra-intestinal lesions



sexual transmission rectal or perirectal abscess

Speciemen for Entamoeba histolytica/dispar

3 stool samples; formalin ethyl acetate conc.



Serology



Pus or drainage from abscess

Laboratory Diagnosis of Entamoeba histolytica; differentiate from dispar

must find ingested RBC's to identify E. histolytica trophs

Entamoeba histolytica/dispar immature cyst:

1-2 nuclei


glycogen mass

Entamoeba histolytica/dispar mature cyst:

  • Nuclei :4 or less
  • peripheral chromatin
  • chromatoidal bar: rounded and elongated

Trophozoites Entamoeba histolytica/dispar

  • 60um in diameter
  • Nucleus: 1 small-centrally located karyosome;peripheral chromatin
  • Ingested RBCs may be seen
  • Unidirectional movement

Cysts Entamoeba histolytica/dispar

  • 10-15um diameter
  • Nuclei: 1-4
  • chromatoid bodies in young cysts

Treatment of Entamoeba histolytica/dispar

Metronidazole


tinidazole


Don't use antidiarrheal

Prevention of E. histolytica/dispar

filter water


boil water


chlorinate water

Where are intestinal amoeba found?

world wide


same location as E. histolytica


person exposed to fecal contamination

What is the diagnostic stage of other amoeba

cysts


trophs

What is the infective stage of other amoeba

cyst

Where do non-pathogen amoebas cyst excyst

In the colon


no ingested RBCs

Clinical manifestations and treatment?

Not considered pathogens


Treatment not recommended

Entamoeba coli cyst:

  • 15-30um diameter


  • 1-8 nuclei


  • irregular peripheral chromatin


  • Chromatid bodies: splinter like

Entamoeba hartmanni Trophozoite

  • 4-12um in size
  • small central karyosome
  • fine, evenly distributed peripheral chromatin
  • non-directional movement

Entamoeba hartmanni cyst

  • 5-10um in diameter
  • 1-4 nuclei
  • small, central karyosome
  • fine, evenly distributed peripheral chromatin

chromotoidal bars rare

Endolimax nana cyst

  • 5-12 um oval
  • 1-4 nuclei
  • large irregular karyosome
  • non peripheral chromatin
  • no chromotoidal bars

Endolimax nana trophozoite

  • 5-12um in size
  • large irregular karyosome
  • no peripheral chromatin
  • may have vacuoles containing bacteria
  • non-directional movement

Iodamoeba butschlii cyst

  • 6-15 um in diameter
  • 1 nuclei large karyosome surround by achromatic granules
  • no peripheral chromatin
  • no chromotoidal bars
  • large glycogen vacuole

Giardia lamblia/intestinals/ duodenalis distribution

worldwide


most common intestinal parasite in the us (flagellate)


called beaver fever

Giardiasis

common cause of waterborne disease


sexually transmitted


Low infective dose in humans (10-25 cysts)


Symptoms begin 1-2 wks after infection


typically late summer and fall

Where is giardiasis confined to?

lumen of the small intestines

Diagnostic stage of Giardia lamblia

cyst


troph

Infective stage of giardia lamblia

ingestion of cyst

Life cycle of Giardia lamblia

cyst fecal-oral route


excyst in sm. intestines


cyst produces 2 trophs


attach to mucosa via ventral sucking


trophs encyst passed in feces

Clinical manifestation of Giardia lamblia

50% asymptomatic


symptomatic:


mal-absorption: steatorrhea

Treatment of Girardia lamblia

metronidazole


tinidazole

What tests can be used for Giardia lamblia

cysts or trophs in stool


enterotest


Antigen detection (Rapid or ELISA)

Staining giardia lambli specimen

wet mount


formalin tube concentrated: iodine wet mount


PVA: trichrome stain

Giardia lamblia cyst

  • 8-12um ellipsoid
  • 4 nuclei
  • longitudinal fibrils
  • parabasal body
  • immediately infective

Giardia lamblia troph

  • pear shaped 9X15 um in size
  • 2 nuclei, 2 axostyles, 8 flagella
  • no peripheral chromatin

Trichomonas vaginalis distribution

worldwide


most common protoza (flagellate) in industrialized countries


higher prevalence in women


higher prevalence in those with more sexual partners

Infective stage of Trichomonas vaginalis

trophozoite

Diagnostic stage of T. vaginalis

trophozoite

Treatment of T. vaginalis

Metronidazole


Tinidazole

Clinical manifestations of T. vaginalis in women

Symptomatic:


vaginal inflammation, yellow frothy discharge, painful urination

Clinical manifestations of T. vaginalis in men

Asymptomatic


possible epididymitis and prostatitis

Specimen collecting in T. vaginalis

wet mount of vaginal secretions or urethral discharge



must see movement of trophs



Pouch TV system

Lab diagnosis of T. vaginalis

trophozoites movement on wet mount


7-30um long tear shaped

Dientamoeba fragilis geographic distribution

worldwide


only a troph stage


associated with helminth infection

Diagnostic/infective stage of Dientamoeba fragilis

trophozoite

Clinical symptoms of DIentamoeba fragilis

asymptomatic


ass. with mucous diarrhea

Lab diagnosis of D. fragilis

  • 1-2 nuclei 40% of time 1 nuclei
  • fragmented nuclear chromatin
  • cytoplasm granulated maybe vacuolated
  • use a permanent stain

Chilomastix mesnili

worldwide, warmer climates


largest flagellate in man


6% or world infected


indicator of fecal contamination

Diagnostic and infective stage of C. mesnili

cyst

Life cycle of C. mesnili

ingest cyst fecal-oral route


cysts excysts in large/small intestines


troph live in cecum/colon


cysts and trophs passed in feces

Diagnosis of C. mesnili

Lemon shaped trophs 6-24um unidirectional movement



Cyst large single nucleus 6-10um coiled filament

Blastocystis hominis distribution

worldwide


one of the most common parasites found in US stool samples

Infective/diagnostic stages of B. hominisn

Thick walled cyst

Balntidium coli distribution

worldwide, warm climates


pigs are an animal resevoir

Clinical presentation of B. coli

asymptomatic may resemble amebiasis

Infective stage B. coli

cyst

Diagnostic stage B. coli

cyst


trophs

Life cycle B.coli

ingest cyst


excyst in sm intestines


troph live in large intestines


trophs encyst to produce infective cyst


mature cyst pass in feces

B. coli diagnostic

trophs in stool or endoscopy samples


Preserved stain


multiple stools

Rapid tests for Giardia and cryptosporidiium

DFA


ELISA


EIA

Nematoda are part of what Phylum?

Nemathelminthes

Trematoda and Cestoda are part of what phylum?

Platyheminthes

Amoebas and Flagellates are part of what class and phylum?

Class: Sarcomastigophora


Phylum: Protozoa


How many people worldwide are infected with soil transmitted heminths?

1 billion people

Where is the larval stage of Nematodes?

can be located outside of the intestinal tract

What location in the body are adult nematodes found?

Intestinal tract

What is the only known host of Hookworm?

Humans

How many people harbor hookworms?

900 million people

Trichuris trichiura host?

Human

Only known host for Pinworm?

Humans

Prevention of Pinworm?

Decontamination of environment and treatment of other family members

Strobila:

entire body

Scolex:

anterior end (head)


used for attachment to intestins

Rostellum:

extreme anterior end

Proglottids

each of the segments; more mature near the posterior end

Next step for Cysticerci in Taenia spp

protoscolexes in the cysticerci envaginate and pass into the small intestines

Largest tapeworm in man?

D. latum

What happens to D. latum embryonated eggs once they're passed in feces?

develop into coracidium larva that are eaten by copepod



The copepod is eaten by a fish; develops into a plerocoid larva (infective stage)

Differentiating symptoms of raw fish ingestion:

Chlonorchiasis: jaundice, liver enlarged, tender, nausea, diarrhea, vomiting, abdominal pain, loss of appetite



D. latum: Irritability, numbness, tingling, increased heart rate, weakness, vague abdominal discomfort

H. nana infective stage

embryonated stage

What happens to unembryonated eggs in F. buski

umbryonated eggs embryonate in the water, miracidia hatch and infect snail. Free swimming cercariae released. Metacercariae on water plant

What stain can you use for S. mansoni?

Modified acid-fast positive


Heavy infection in males with S. haematobium causes

scrotal lymphadenopathy

Trophozoite characteristics

feeding


motile


replication

Cyst characteristics

Resistant


Infective


(encystment/excystation)

Fresh specimens are needed for/ direct wet mount?

motility for trophs (amebae, flagellates, ciliates, Strongyloides)

ELISA is for

E. histolytica

Formalin is used for?

Concentrates for eggs, cysts, larvae