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Chocolate agar.

Growth for Neisseria and Haemophilus.

Loffler's medium.

Growth for Corynebacterium diphtheriae.

Maneval's stain.

Klebsiella pneumoniae.

Dorner's/spore stains.

Bacillus and Clostridium.

Acid-fast stain/Ziehl-Neeson method.

Mycobacterium tuberculosis and leprae (because of high lipid mycolic acid in cell wall).

Neisser's stain.

Granules.

Selective media.

Promotes growth of desirable microbes and inhibit the growth of others.

Cetrimide agar (what media and what for?).

Selective media for Pseudomonas aeruginosa (G-negative).




Has cetrimide (desinfectant).

Sabourauda agar (what media and what for?).

Selective media for fungi isolation (e.g. Candida albicans and Apergillus niger).




Has antibiotic and low pH.

What are differential media?

Substances that cause some bacteria to take on a different appearance.

Chapman agar (mannitol).

Differential media for Staphylococcus.

MacConkey agar (lactose).

Differential media for Gram-enteric rods (e.g. Escherichia coli).

Medically important bacteria that cannot be cultured in media.

- Treponema pallidum (testicles of live rabbits).


- Mycobacterium leprae (armadillo.


- Chlamydia and Chlamydophila, Rickettsia (tissue culture or chicken embryo like viruses).

The 5 I's.

1. Inoculation.


2. Isolation.


3. Incubation.


4. Inspection.


5. Identification.

Semisolid media used for what?

1. Check motility of bacteria.


2. Promote anaerobic growth.


3. Fermentation studies.

Colony.

Group of several million bacteria (clones) derived from one bacterial cell.

CFU/mL is the same as...?

bacteria/mL.

Heterotrophs.

Bacteria that require organic carbon for growth e.g. simple sugars (C,N,K,S).




All clinically important bacteria fall under this category.

Haemophilus influenzae growth in medium via?

Hemin and NAD.

Mycoplasmas growth in medium via?

Cholesterol.

Most pathogenic bacterias' optimum pH.

Neutralophiles @ 7-7,3.

Vibrio cholerae's optimum pH.

Alkaliphile @ 8,5-9,5.

Alkaliphiles.

Optimum pH >8,5.

Acidophiles.

Optimum pH <5,5.

What organisms tolerate moderate acid pHs?

Fungi (yeast and molds) and some bacteria e.g. Lactobacilli and Streptococci.

Mesophiles optimum temperature.

35 degrees Celsius.

Psychrophiles optimum temperature.

5 degrees Celsius.

Thermophiles optimum temperature.

70 degrees Celsius.

Aerobes.

Facultative anaerobes.

Anaerobes.

Sterilization.

Killing all living entities and endospores.

Alfa hemolysis.

Partial hemolysis in blood agar. RBCs remain intact, but hemoglobin components' color are altered.

Beta hemolysis.

Complete hemolysis in blood agar. RBCs have become lysed and hemoglobin components completely colorless.

Gamma hemolysis.

No hemolysis in blood agar. RBCs remain intact and unaltered.

Wet mounts. What is it, and what's the purpose?

Short-term mounts for fresh, living preparations.




Study if a microorganism is motile or not (possess flagella). Also permits undistorted view of arrangement, size, shape and color.

Smears.

Long-term mounts that are fixed and stained preparations.

Basic (cationic) stains.

Positively charged stain that binds to negatively charged compounds of bacterial cell wall.




Methylene blue, crystal violet, safranin and basic fuchsin.

Acidic (anionic) stains.

Negatively charged stain that stains the background.




Nigrosin and india ink.

What is this? Purpose?

What is this? Purpose?

Heat fixation.




- Kill the bacterial cells.


- Attach/fix them to the glass surface.


- Preserve morphology but not internal structures.

Other fixation methods than heat.

Alcohol and acetone.

Gram-positive bacteria.

- Staphylococcus aureus.


- Streptococcus pneumoniae.


- Bacillus anthracis.

Gram-negative bacteria.

- Haemophilus influenzae.


- Escherichia coli.


- Neisseria mengitidis.

Etiologic agent of tuberculosis.

Mycobacterium tuberculosis.

Etiologic agent of leprosy; Hansen's disease.

Mycobacterium leprae.

Etiologic agent of syphilis.

Treponema pallidum.

Negative stain pro.

More accurate cell size determination because of no heat fixation (shrinkage).




Accentuated capsule.




Useful for studying spirochetes (e.g. T. pallidum).

Endospores formed by gram-positive bacilli.

- Bacillus (aerobic bacilli).


- Clostridium (anaerobic bacilli).

Species and type of spore.

Species and type of spore.

Bacillus spp.




Subterminal spore.

Species and type of spore.

Species and type of spore.

Bacillus spp.




Central spore.

Species and type of spore.

Species and type of spore.

Clostridium botulinum.




Subterminal spore/"tennis racquet".

Species and type of spore.

Species and type of spore.

Clostridium tetani.




Terminal spore/"drumstick".

Species and type of spore.

Species and type of spore.

Clostridium spp.




Central spore.

Gram stain does not stain what?

Endospores. However, the vegetative cell is, thus showing the presence of endospore.

Endospores. However, the vegetative cell is, thus showing the presence of endospore.

Maneval's stain stains what structures?

Background and cell, but NOT capsule.

Flagella.

Fine, threadlike organelles of locomotion.



Peritrichous.



Iodotrichous.

Amphitrichous.



Monotrichous.

Irregular or variable in shape bacteria.

Pleomorphic bacteria.



Vibrio/incomplete spiral.



Spirillium/spiral.



Club-shaped.



Cocco-bacilli.



Bacillus/rod-shaped.



Coccus/spherical.



Listeria.



Bacillus spp.



Corynebacterium diphtheriae.



Candida albicans (yeast).




ALL YEAST AND YEAST-LIKE FUNGI ARE GRAM-POSITIVE.

Resolving power of a light microscope.

0,2 μm.

Resolving power.

The ability to distinguish two points as separate ones.

Microorganisms.

Living organisms too small to be seen with the naked eye.

Naked eye resolution limit.

ca. 0,1 mm.

Superbug.

Antibiotic-resistant bacteria aka MRSA.

How do microorganisms keep the earth's ecosystem in balance?

- Account for >50% of the worlds' photosynthesis.


- Decomposition of dead organic matter and nutrient recycling.


- Fixation of free nitrogen (symbiosis with plants).


- Make possible cycles of C, O2, N and S.


- Regulate temperature of the earth.

In what part of our lives are we free of microbes?

Fetal life.

How many bacteria do we have in and on our body compared to our cells?

10x more.

Etiologic agent of plague.

Yersinia pestis.

The study of microorganisms which affect human health.

- Pathogenesis.


- Epidemiology.


- Diagnosis.


- Prophylaxis.

Mutualism.

Both host and microbe benefit.

Commensalism.

Host not harmed, commensal benefits.

Parasitism.

Host harmed, parasite benefits.

Carrier state.

Asymptomatic individual who is host to pathogenic microorganism and has the potential of transmitting it to others.

Etiologic agent of typhoid fever.

Salmonella typhi.

Cellular and prokaryotic (no nucleus).

Bacteria and archaea.


Cellular and eukaryotic (with nucleus).

Unicellular: Fungi (yeasts) and protists.




Multicellular: Fungi (molds) and worms.

Noncellular and only RNA.

Viroid.

Noncellular and only protein.

Prions.

Noncellular with proteins + RNA or DNA.

Virus.

Phylogenetics.

Evolution of the organism.

Phenotype.

The physically seen traits (shape, size).

Eukaryotic cell.




1. Cell wall.


2. DNA.


3. Histones.


4. First amino acid in protein synthesis.


5. Ribosomes.


6. Cell division.


7. Cell membrane.

1. Plants (cellulose) and fungi (chitin).


2. Linear.


3. Present.


4. Methionine.


5. 80S.


6. Mitosis.


7. Sterols.

Prokaryotic cell.




1. Cell wall.


2. DNA.


3. Histones.


4. First amino acid in protein synthesis.


5. Ribosomes.


6. Cell division.


7. Cell membrane.

1. Bacteria (peptidoglycan) and archaea.

2. Circular.


3. Only in archaea.


4. Formylmethionine (bacteria) and methionine (archaea).


5. 70S.


6. Binary fission.


7. No sterols (except mycoplasmas).



Eukaryotic organelles.




1. What are they?


2. Cell wall.


3. DNA.


4. Histones.


5. First amino acid in protein synthesis.


6. Ribosomes.


7. Cell division.

1. Mitochondria and chloroplasts.


2. No.


3. Circular.


4. No.


5. Formylmethionine.


6. 70S.


7. Binary fission.

The idea of eukaryotic species.

Based on sexual reproduction.

Strain.

Subset of a bacterial species differing from other bacteria of the same species by some minor but identifiable difference.

Streptococcus pneumoniae is the leading cause of what?

- Community-acquired pneumonia.


- Meningitis.


- Upper respiratory tract infections.


- Sinusitis.


- Otitis media.

Mycoplasma.




1. What is it?


2. Typical cell wall.


3. Pathogen.


4. Reproduction.


5. Size range.


6. Shape.


7. Stain.

1. Bacteria.


2. No cell wall.


3. Extracellular pathogens.


4. Asexually via binary fission.


5. Smallest 0,2 μm.


6. Pleomorphic.


7. Neither Gram-positive or negative.

Rickettsia.




1. What is it?


2. Typical cell wall.


3. Pathogen.


4. Reproduction.


5. Size range.


6. Shape.


7. Stain.

1. Bacteria.


2. Typical cell wall.


3. Obligate intracellular pathogen.


4. Asexually via binary fission.


5. Very small (<0,1 μm).


6. Coccobacilli.


7. Neither Gram-positive or negative.

Chlamydia.




1. What is it?


2. Typical cell wall.


3. Pathogen.


4. Reproduction.


5. Size range.


6. Shape.


7. Stain.

1. Bacteria.


2. Atypical cell wall.


3. Obligate intracellular pathogen.


4. Specific life cycle (two stages).


5. Very small (<0,1 μm).


6. Coccobacilli.


7. Neither Gram-positive or negative.

Walking pneumonia/pneumonia with atypical course.

Mycoplasma pneumoniae.

Nonspecific infections of the urogenital tract.

Mycoplasma hominis and Ureaplasma urealyticum.

Etiologic agent of typhus.

Rickettsia prowazekii and typhi, transmitted by blood-sucking arthropods.

Etiologic agent of Rocky Mountain spotted fever.

Rickettsia rickettsii, transmitted by ticks.

Chlamydia eye serotypes.

A-C.

Chlamydia genital serotypes.

D-K.

Chlamydia L1, L2, L3 serotypes.

Lymphogranuloma venereum (LGV).

Etiologic agent of psittacosis or ornithosis.

Chlamydophila psittaci.

How do yeasts grow, and how do they reproduce?

They grow as single cells (unicellular) and reproduce mainly by asexual budding.

How do molds grow, and how do they form?

Molds grow as long filaments (hyphae) and form a mat-like structure (mycelium). Multicellular.

Dimorphic fungi.

Form different structures at different temperatures e.g. molds at room temperature and yeast at body temperature.

Two main characteristics differentiating fungi from other eukaryotes (e.g. protozoa).

- Rigid cell wall containing chitin or glucan.


- Cell membrane with ergosterol (instead of cholesterol).

Viroids definition.

Noncoding ssRNA molecules that interfere with cellular RNA as antisense RNA.

The only significant human pathogen structurally related to the viroids.

Hepatitis D virus.

Prions definition and characteristic.

Proteinaceous Infectious Particles.




- No nucleic acids.


- Made of proteins.




- Diseases are disorders of protein conformation.


- Occurs as infectious, sporadic or genetic.


- Does not elicit inflammatory or immune response.


- Untreatable and universally fatal.




Causes degeneration of CNS via Transmissible Spongiform Encephalopathies (TSE).

What type of cell is this? Name the parts.

What type of cell is this? Name the parts.

Bacteria.

Bacteria.

What type of cell is this? Name the parts.

What type of cell is this? Name the parts.

Prokaryotic cell.
Prokaryotic cell.

What type of cell is this? Name the parts. 

What type of cell is this? Name the parts.

Eukaryotic cell. 

Eukaryotic cell.

No membrane-delimited compartments.

- Nucleus.


- Golgi apparatus.


- Mitochondria/chloroplasts.


- Endoplasmic reticulum.

MENG

Bacterial essential components.

- Cytoplasm.


- Ribosomes 70S.


- Nuclear area (nucleoid) containing DNA.


- Cell wall (except mycoplasmas).


- Plasma membrane.

Bacterial nonessential components.

- Fimbriae.


- Flagella.


- Plasmid.


- Inclusion.


- Mesosomes.


- Capsule.

**** Friends Puler Ikke Med Condom.

Bacterial cell wall main component and function.

Peptidoglycan (murein, mucopeptide). Gives the bacteria its' rigid structure.

Gram+ bacteria cell wall characteristic.

Thick cell wall layer of peptidoglycans, accounting for 80% of dry cell wall mass.

Gram- bacteria cell wall characteristic.

Thin cell wall layer of peptidoglycans, covered by an external outer membrane (OM), accounting for 10% of dry cell wall mass.

Penicillin function.

Disrupt murein synthesis (aka cell wall synthesis).

What enzyme digests murein (peptidoglycan) and proteins?

Lysozyme.

Virulence factors of Gram-positive bacteria. What and where?

- Teichoic acids on murein/cell wall.


- Lipoteichoic acids anchored on CM.


- Cell-associated proteins.

Severity of a disease caused by a particular pathogen depends on what?

Its' virulence. The capability to establish itself in the host and cause damage.

Common cause of pharyngitis and rheumatic fever.

Streptococcus pyogenes.




Caused by antibodies that attack S. pyogenes' M protein cross-react with myocardial cells.

Facultative intracellular pathogen.

Listeria monocytogenes.




Internalin binds to host intestinal epithelium.

Outer membrane of Gram-negative bacteria consists of what? What is shed from the bacteria?

Porins, lipoproteins, transport protein and LIPOPOLYSACCHARIDE (LPS).




LPS is shed from the bacteria.

Vancomycin use. Function?

ONLY AGAINST GRAM-POSITIVE BACTERIA.




Because in Gram-negative, its' molecular structure is far too large to cross the outer membrane.




Inhibition of cell wall synthesis by binding to D-alanine D-alanine.

Responsible for the toxic effect of LPS.

Lipid A.

Lipooligosaccharide (LOS) present where, and what is the characteristic?

Present in:


- Haemophilus.


- Neisseria.


- Bordetella.


- Vibrio.


- Acinetobacter.




Lacks O antigen portion of LPS and is readily shed from bacteria.

LPS category and function.

Endotoxin that elicits septic shock by activating B cells to induce Mø, DC to release IL-1 and TNF.

Important enzyme in periplasmic space.

Beta-lactamase.




Also proteases, phosphatases, lipases and nucleases.




They are involved in transport, degradation and synthesis.

Porins.

Proteins that form pores in OM to allow passage of essential substances.




(This, for instance, gives Gram-negative bacteria protection against some antibiotics).

What is a beta-lactam antibiotic, and what mechanism is resistant against it? Where do Gram-positive and Gram-negative bacteria use this mechanism?

Penicillin. Beta-lactamase inactivates it.




Gram-positive inactivate beta-lactam drugs extracellularly.




Gram-negative inactivate beta-lactam drugs in the periplasmic space.

What Gram-stained bacteria, and what are the parts?

What Gram-stained bacteria, and what are the parts?



What Gram-stained bacteria, and what are the parts?

What Gram-stained bacteria, and what are the parts?



Function of bacterial cell wall.

- Determines the shape.


- Provides structural support to protect from collapsing because of changes in osmotic pressure.


- Plays an important role in cell division.


- Protects against antibodies, antibiotics and lysozymes.


- Some components are virulence factors.

L-forms of bacteria. Characteristic?

Bacteria with cell wall defects. They are totally resistant to antibiotics which block synthesis of murein.




Can be through mutation, lysozyme or penicillin.

Gram-negative L-form.

Gram-negative L-form.

Spheroplast.

Gram-positive L-form.

Gram-positive L-form.

Protoplast.

Mycoplasma cell membrane stabilized by? Function?

Sterols. Resistant to lysis.

Mycobacteria cell wall structure.

Peptidoglycan layer surrounded by a wax like coat of mycolic acid and other lipids.

Etiologic agent for diphtheria.

Corynebacterium diphtheriae.

Bacterial cytoplasmic membrane (CM).

Lipid bilayer structure similar to the structure of eukaryotic membranes, but contain no sterols (except mycoplasma).



Plays an important role in cell division.

Enzymes in formation of the peptidoglycan.

Penicillin-Binding Proteins (PBPs).

B-lactam antibiotics resistance mechanisms.

- PBP modification (MRSA).


- B-lactamase production.

Mesosomes.

- Complex invaginations of CM.


- Play a role in cell division.


- Site for respiratory enzymes.

Polysaccharide coat. Function?

Glycocalyx. Coating of macromolecules (polysaccharides) to protect the cell.




Can be slimy or gelatinous (capsule).




Can also be a virulence factor!

Biofilm. Examples?

A structured community of bacterial cells embedded in a self-produced polymer matrix attached to an inert surface or living tissue.




Dental plaque is an example.

Host-actin polymerization.

Bacteria motility inside eukaryotic cell.

Structure of bacterias that most commonly cause meningitis.

Encapsulated.

Fimbriae (pili) found where? Function?

Gram-negative bacteria.




1. Adherence (virulence).


2. Sex pili --> Transfer of DNA during conjugation.

Reproductive spores.

Fungal spores.




NOT BACTERIAL SPORES.

Most resistant microbial entities.

Endospores.

Endospores characteristics.

- Highly dehydrated.


- High content of calcium.


- Special molecules (e.g. dipicolinic acid).


- Surrounded by keratin-like coat.

Goal of sterilization.

Endospore destruction.

Etiologic agent for anthrax.

Bacillus anthracis.

Zoonosis.

Infectious disease naturally transmissible from animals to humans.

Tetanus toxin.

Clostridium tetani - Prevents muscle relaxation (because of neurotoxin tetanospasmin).

Botulinum toxin.

Clostridium botulinum - Prevents muscle contraction.

The most powerful toxin known to man.

Botulinum toxin aka Botox.

Haploid bacteria.

Bacteria usually only has one single circular chromosome.

Linear chromosomed bacteria.

Borrelia burgdorferi and Streptomyces spp.

Etiologic agent for lyme disease.

Borrelia burgdorferi.

Humans' chromosome size. How much of this is the genome?

3000 Mb. 750 Mb.

Operon.

A coordinated set of genes, all of which are regulated as a single unit.

Bacterial mRNA.

Polycistronic.




Numerous proteins are coded for on the same mRNA.

Bacterial operon negative control.

Repressor protein binds to operator, preventing RNA polymerase from binding properly to the promoter sequence.

Bacterial operon positive control.

Activator protein binds to operator, stabilizing the RNA polymerase binding to the promoter sequence.

Region of the cytoplasm containing the chromosome.

Nucleoid.

Enzymes responsible for DNA supercoiling.

Gyrases/Topoisomerases.

Bacterial virus.

Bacteriophage.

Mobilomes.

Plasmids, transposons and bacteriophages.

Horizontal Gene Transfer (HGT). How does this happen?

Bacteria that can pass their genes not only to offspring, but also to other microbes.




- Conjugation (via plasmids).


- Transduction (via bacteriophages).


- Transformation (acquisition of "naked" DNA from environment).

Plasmids characteristics.

- Replicate independently from the chromosome.


- Can be a part of the chromosome (episomes).

Lysogenic cycle (lysogeny) characteristic.

The interaction with bacteriophage does not cause cell disruption.




That, compared to the lytic cycle, does.

Lysogenic conversion.

When a bacteria acquires a new feature (e.g. virulence) from its' prophage.

Etiologic agent for cholera.

Vibrio cholerae.

Etiologic agent for erythrogenic toxin; Scarlet fever.

Streptococcus pyogenes.

What are they, and what is the stain? 

What are they, and what is the stain?

Ernst-Babes bodies with Neisser's stain.

Diphtheria toxin production is dependent on what?

Presence of lysogenic beta-phage.

Antibiotics mechanisms of action.

1. Cell wall.


2. Protein synthesis (50S and 30S subunit).


3. DNA + RNA.


4. Folic acid synthesis.


5. Cell membrane.

Generation time.

Doubling time. The time interval between two subsequent binary fissions.

All bacteria which can live in the presence of oxygen has what kind of enzyme, and what is its' function?

Superoxide dismutase (SOD); catalyzes the reduction of superoxide anions to H2O2.

Both aerobic and anaerobic contain what kind of enzyme, and what is its' function?

Catalase; decomposes H2O2.

Polymers of ribitol or glycerol phosphate.

Teichoic acid.

The wall formed during prokaryotic cellular division.

Septum.

Patients with kidney stones usually have this condition caused by these bacteria.

High levels of urease producing bacteria, such as Proteus spp. and Klebsiella spp.

Microbial succession.

Process in which organisms previously unable to colonize the original site are now provided with an environment suitable for their growth.

Quorum sensing.

Bacteria are able to sense the presence of other bacterial cells.

Amensalism (antagonism) is the same as?

Parasitism.

Microorganisms cannot alter the pH of their surroundings (T/F).

True.

Rickettsia and Chlamydia cannot be cultured on artificial media (T/F).

True.

Contains RNA in its' genome.

HIV.

Smallest group of bacteria include...?

Rickettsia, chlamydia and mycoplasma.

Viral enzyme that makes a DNA copy of an RNA molecule.

Reverse transcriptase.

What must be present during transduction?

Bacteriophage.

Pieces of DNA that have the ability to move from one site to another on the chromosome.

Transposons and SI.

Cocci arranged in "grape-like" clusters.

Staphylococci.

The form of bacterial virus which is covalently inserted into its' hosts' DNA.

Prophage.

Has mycolic acid on cell wall and identified with acid-fast stain.

Nocardia.

Recombination that does not require homologous sequences and is utilized by mobile genetic elements that move about chromosomes.

Replicative recombination.

Mediates chemotaxis towards nutrients.

Flagella.

One-way transfer of genetic information.

Conjugation.

Grow best in the presence of increased concentrations of CO2.

Capnophiles e.g. Haemophilus influenzae.

Identification or typing of bacteria by specific antibodies.

Serotyping.

Protein coat of a virus.

Capsid.

Viruses that contain an RNA genome, which is converted to a DNA molecule as they are replicated.

Retroviruses.

Space between cytoplasmic membrane and the outer membrane of Gram-negative bacteria.

Periplasmic space.

Most common organisms found on human skin.

Staphylococcus epidermidis.

Major site of Bacteriodes fragilis.

Colon.

Common site for Staphylococcus aureus.

Nose.

Attenuated toxin.

Diphtheria toxoid.

Thread-like filaments forming the mycelium of the fungus.

Hypha.

Bacterial membrane contains what?

Hopanoids, etholamine and integral proteins.

Gram staining procedure.

Crystal violet, Gram's iodine, Alcohol and Fuchsin.

Bacterial growth phases.

- Lag phase.


- Growth phase.


- Stationary phase.


- Death phase.

Viral infection process.

- Attachment.


- Penetration.


- Uncoating.


- Replication.


- Assembly.


- Release.

Causes the creation of new types of influenza virus.

Reassortment.

Faulty protein.

Missense.

Incomplete protein.

Nonsense.

Normal protein.

Silent.

How many genes does Escherichia coli code?

About 5000 genes.

Group that only includes prokaryotic cells.

Bacteria.

Size range typical for medically important bacteria.

0,2-8 micrometers.

What is being compared during DNA hybridization studies of two bacteria?

Similarity of nucleotide sequences.

Catalase is important in what genera?

Staphylococcus and Streptococcus.

Part of influenza virus playing part in attaching to host cells.

Hemagglutinins.

RNA viruses multiply where?

Cell cytoplasm.

DNA viruses multiply where?

Cell nucleus.

Nucleic acid configuration NOT found in viral genomes.

Segmented double-stranded DNA.

HIV is an oncovirus (T/F).

False.

What fungi causes endemic mycoses?

Aspergillus and Mucor.

How does a capsule increase the virulence factor?

Helps bacteria evade phagocytosis.

Capsule of Bacillus anthracis composed of what?

Amino acids.

Virus with oncogenic properties in humans.

Epstein-Barr Virus (EBV).

Virion meaning.

Infectious virus particles.

Serotyping is based on what?

Antigen-antibody reactions.

Ergosterol is found where?




In the cell membrane of what?

Apergillus flavus.




Fungi.

Bacterial mRNA molecule that encodes for several proteins.

Polycistronic mRNA.

LPS is a protein (T/F).

False.

Where is endotoxins found?

In Gram-negative bacteria.